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OEM Manufacturer Oxford Nanopore Service - circRNA sequencing-Illumina – Biomarker

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OEM Manufacturer Oxford Nanopore Service - circRNA sequencing-Illumina – Biomarker Detail:

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Contamination	Amount
OD260/280≥1.7-2.5;OD260/230≥0.5-2.5;	For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation	Limited or no protein or DNA contamination shown on gel.	Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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Nanopore Sequencing Cost

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Spatial Single Cell Rna Seq

This company has the idea of "better quality, lower processing costs, prices are more reasonable", so they have competitive product quality and price, that's the main reason we chose to cooperate.

By Heloise from Spain - 2017.02.18 15:54

The customer service reprersentative explained very detailed, service attitude is very good, reply is very timely and comprehensive, a happy communication! We hope to have a opportunity to cooperate.

By Eden from Bangkok - 2017.08.28 16:02