Kutsatizana konse kwa genome bisulfite
Ubwino wa Utumiki
● Pulatifomu yathunthu: perekani ntchito imodzi yabwino kwambiri kuyambira pakukonza zitsanzo, kumanga laibulale, kutsata kusanthula kwa bioinformatics.
● Kulondola kwakukulu: Ukadaulo wokhwima wa kutembenuka kwa methylation ungathe kusanthula molondola mkhalidwe wa methylation wa C base imodzi.
● Kufalikira kwakukulu: kuzindikira malo a methylation pamlingo wa genome-wide.
● Kubwereza kwabwino: kukonza kokhazikika, koyenera kusanthula kofananitsa pakati pa zitsanzo zambiri.
Zitsanzo Zofotokozera
|
nsanja
|
Werengani Utali
|
Njira Yotsatirira
|
| Ilumina | Mtengo wa 150PE | 30X, zofananira zitatu zamoyo, zokhala ndi ma genome apamwamba kwambiri |
Zitsanzo Zofunika
|
Mtundu Wachitsanzo
|
Mtengo (μg)
|
Conc.(ng/μl)
|
Kuchuluka (μl)
|
Chiyero
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Kuwonongeka kochepa ndi kuipitsidwa
|
Kuyenda kwa Ntchito Yantchito
Kupereka zitsanzo
Kutulutsa kwa DNA
Kumanga laibulale
Kutsata
Kusanthula deta
Kutumiza kwa data
Bioinformatics
1.Genome-wide DNA methylation distribution
2. Ndemanga pa dera la CGI lomwe lili ndi methylated kwambiri
