Kuzindikira kwakukulu kwa chikhomo- Ukadaulo wotsogola wotsogola kwambiri umathandizira SLAF-Seq kupeza ma tag mazana masauzande mu genome yonse.
Kudalira kochepa pa genome- Itha kugwiritsidwa ntchito ku mitundu yokhala ndi kapena popanda genome.
Flexible scheme design- Single-enzyme, ma enzyme awiri, chimbudzi cha ma enzyme ambiri ndi mitundu yosiyanasiyana ya michere, zonse zitha kusankhidwa kuti zikwaniritse cholinga chofufuza kapena mitundu yosiyanasiyana.Kuunikiratu mu silico kumagwiritsidwa ntchito kutsimikizira kapangidwe kabwino ka enzyme.
Kuchita bwino kwa enzymatic chimbudzi- Kuyesera kusanachitike kunachitika kuti kukhathamiritse mikhalidwe, zomwe zimapangitsa kuyesa kokhazikika kukhala kokhazikika komanso kodalirika.Kutolere bwino kwa zidutswa kumatha kukwaniritsa 95%.
Ma tag a SLAF omwe amagawidwa mofanana- Ma tag a SLAF amagawidwa mofanana mu ma chromosome onse kumlingo waukulu, kukwaniritsa avareji ya 1 SLAF pa 4 kb.
Kupewa koyenera kubwereza- Kubwerezabwereza mu data ya SLAF-Seq kumachepetsedwa mpaka 5%, makamaka mumitundu yokhala ndi kubwereza kwakukulu, monga tirigu, chimanga, ndi zina.
Zochitika zambiri-Kupitilira 2000 ntchito zotseka za SLAF-Seq pazamoyo mazanamazana zomwe zimaphimba zomera, zoyamwitsa, mbalame, tizilombo, zamoyo zam'madzi, ndi zina zambiri.
Ntchito yodzipangira yokha bioinformatics- Integrated bioinformamatic workflow for SLAF-Seq idapangidwa ndi BMKGENE kuti zitsimikizire kudalirika komanso kulondola kwa zotsatira zomaliza.
nsanja | Conc.(ng/gl) | Zonse (ug) | OD260/280 |
Illumina NovaSeq | >35 | >1.6(Chigawo> 15μl) | 1.6-2.5 |
Kutsata kuya: 10X/Tag
Kukula kwa Genome | Analimbikitsa SLAF Tags |
<500Mb | 100K kapena WGS |
500 Mb-1 Gb | 100 k |
1 Gb -2 Gb | 200 k |
Ma genome akuluakulu kapena ovuta | 300-400K |
Mapulogalamu
| Analimbikitsa Chiwerengero cha Anthu
| Kutsata ndondomeko ndi kuya
| |
Kuzama
| Nambala ya Tag
| ||
GWAS
| Nambala yachitsanzo ≥200
| 10x pa
|
Malinga ndi kukula kwa genome
|
Kusintha kwa Genetic
| Anthu payekhapayekha gulu ≥ 10; zitsanzo zonse ≥ 30
| 10x pa
|
Chidebe: 2 ml centrifuge chubu
Pazitsanzo zambiri, timalimbikitsa kuti musasunge ethanol.
Zitsanzo zolembetsera: Zitsanzo ziyenera kulembedwa momveka bwino komanso zofanana ndi zomwe zatumizidwa.
Kutumiza: Owuma-Izi: Zitsanzo ziyenera kulongedzedwa m'matumba kaye ndikukwiriridwa mu ayezi wouma.
1. Ziwerengero za zotsatira za mapu
2. Kukula kwa chikhomo cha SLAF
3. Mawu osinthika
Chaka | Journal | IF | Mutu | Mapulogalamu |
2022 | Kulankhulana kwachilengedwe | 17.694 | Genomic maziko a giga-chromosomes ndi giga-genome ya mtengo peony Paeonia ostii | SLAF-GWAS |
2015 | Phytologist watsopano | 7.433 | Zolemba zapakhomo zimakhazikitsa madera a genomic ofunika kwambiri mu agronomic soya | SLAF-GWAS |
2022 | Journal of Advanced Research | 12.822 | Ma genome-wide artificial introgressions of Gossypium barbadense into G. hirsutum wonetsani malo apamwamba kuti muwongolere nthawi imodzi yamtundu wa thonje komanso zokolola makhalidwe | SLAF-Evolutionary genetics |
2019 | Chomera cha Molecular | 10.81 | Population Genomic Analysis ndi De Novo Assembly Reveal Origin of Weedy Mpunga ngati Masewera a Chisinthiko | SLAF-Evolutionary genetics |
2019 | Nature Genetics | 31.616 | Mndandanda wa ma genome ndi mitundu yosiyanasiyana ya ma carp wamba, Cyprinus carpio | Mapu a SLAF-Linkage |
2014 | Nature Genetics | 25.455 | Ma genome a mtedza wolimidwa amapereka chidziwitso cha legume karyotypes, polyploid chisinthiko ndi kubzala mbewu. | Mapu a SLAF-Linkage |
2022 | Plant Biotechnology Journal | 9.803 | Kuzindikirika kwa ST1 kumawonetsa kusankha komwe kumakhudza kukwera kwa mbeu ndi mafuta ochulukirapo panthawi yoweta soya | Kukula kwa SLAF-Marker |
2022 | International Journal of Molecular Sciences | 6.208 | Kuzindikiritsa ndi DNA Marker Development for Wheat-Leymus mollis 2Ns (2D) Kusintha kwa Disomic Chromosome | Kukula kwa SLAF-Marker |