Small RNA sequencing-Illumina
Mawonekedwe
● Kusankha kukula kwa RNA isanayambe kukonzekera laibulale
● Kusanthula kwa Bioinformamatic kunakhazikika pa kulosera kwa miRNA ndi zolinga zawo
Ubwino wa Utumiki
●Kusanthula kwathunthu kwa bioinformatics:kupangitsa kuzindikirika kwa ma miRNA odziwika komanso atsopano, kuzindikiritsa zolinga za miRNAs ndi mawu ofananirako ogwira ntchito ndikulemeretsa ndi ma database angapo (KEGG, GO)
●Kuwongolera Kwabwino Kwambiri: Timagwiritsa ntchito mfundo zowongolera pamagawo onse, kuyambira pakukonza zachitsanzo ndi laibulale mpaka kutsatizana ndi bioinformatics.Kuyang'anitsitsa mosamalitsa kumeneku kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.
●Thandizo la Post-Sales: Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa.Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
●Katswiri Wazambiri: Ndi mbiri yakutseka bwino mapulojekiti angapo a sRNA okhala ndi mitundu yopitilira 100 m'magawo osiyanasiyana ofufuza, gulu lathu limabweretsa zokumana nazo zambiri pantchito iliyonse.
Zitsanzo Zofunika ndi Kutumiza
| Library | nsanja | Deta yovomerezeka | Zithunzi za QC |
| Kukula kwasankhidwa | Illumina SE50 | 10M-20M imawerengedwa | Q30≥85% |
Zitsanzo Zofunika:
Nucleotides:
| Conc.(ng/μl) | Mtengo (μg) | Chiyero | Umphumphu |
| ≥80 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel. | RIN≥6.5; 5.0≥28S/18S≥1.0; kukwera kochepa kapena kopanda koyambira |
● Zomera:
Muzu, tsinde kapena petal: 450 mg
Tsamba kapena Mbewu: 300 mg
Chipatso: 1.2 g
● Chinyama:
Mtima kapena matumbo: 450 mg
Viscera kapena Ubongo: 240 mg
Minofu: 600 mg
Mafupa, Tsitsi kapena Khungu: 1.5g
● Matenda a nyamakazi:
Kulemera kwake: 9g
Krustacea: 450 mg
● Mwazi wonse:2 machubu
● Maselo: 106 maselo
● Seramu ndi Plasma:6 mL pa
Kutumiza Kwachitsanzo Kovomerezeka
Chotengera:
2 ml centrifuge chubu (Tin zojambulazo sizovomerezeka)
Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3;B1, B2, B3...
Kutumiza:
1.Dry-ice: Zitsanzo ziyenera kuikidwa m'matumba ndikukwiriridwa mu ayezi wouma.
2.RNAstable machubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa mu firiji.
Kuyenda kwa Ntchito Yantchito
Kuyesera kupanga
Kupereka zitsanzo
Kusintha kwa RNA
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Bioinformatics
Kuzindikiritsa miRNA: kapangidwe ndi kuya
Kufotokozera kosiyana kwa miRNA - magulu a hiearchical clustering
Kufotokozera kogwira ntchito kwa chandamale cha ma miRNA owonetsedwa mosiyanasiyana
Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene 'sRNA yotsatizanatsatizana ndi zofalitsa.
Chen, H. et al.(2023) 'Matenda a Viral amalepheretsa saponin biosynthesis ndi photosynthesis ku Panax notoginseng', Plant Physiology and Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al.(2023) ' Chomera cha FYVE chokhala ndi puloteni ya FREE1 chimagwirizana ndi zigawo za microprocessor kuti zipondereze miRNA biogenesis ', Malipoti a EMBO, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al.(2023) 'The MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Targeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) in the Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al.(2018) 'Integrated Analysis of MiRNA and Genes Associated With Meat Quality Amasonyeza kuti Gga-MiR-140-5p Imakhudza Kuyika kwa Mafuta mu Nkhuku', Cellular Physiology ndi Biochemistry, 46 (6), pp. 2421-2433.doi: 10.1159/000489649.
