PacBio sequencing platform ndi nsanja yowerengera nthawi yayitali, yomwe imadziwikanso kuti ndi imodzi mwaukadaulo wa Third-Generation Sequencing (TGS).Ukadaulo wapakatikati, single-molecule real-time(SMRT), imathandizira kupanga zowerengera ndi makumi a kilo-base kutalika.Pansi pa "Sequencing-by-Synthesis", kusamvana kwa nucleotide imodzi kumatheka ndi Zero-mode waveguide(ZMW), pomwe voliyumu yochepera pansi (malo opangira ma molecule), imawunikiridwa.Kuphatikiza apo, kutsatizana kwa SMRT kumapewa kukondera kwanthawi yayitali mu dongosolo la NGS, chifukwa njira zambiri zokulira za PCR sizofunikira pakumanga laibulale.