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Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)

Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS) Chithunzi Chowonetsedwa
  • Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)
  • Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)
  • Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)

Kafukufuku wa DNA methylation nthawi zonse wakhala mutu wovuta kwambiri pa kafukufuku wa matenda, ndipo umagwirizana kwambiri ndi maonekedwe a jini ndi makhalidwe a pheno-typic.RRBS ndi njira yolondola, yothandiza komanso yachuma pa kafukufuku wa DNA methylation.Kulemeretsa kwa zigawo za zilumba za CpG ndi enzymatic cleavage (Msp I), kuphatikizidwa ndi kutsatizana kwa Bisulfite, kumapereka kuzindikira kwakukulu kwa DNA methylation.

Pulatifomu: Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Zotsatira za Demo

Mafotokozedwe a Utumiki

Platform: NovaSeq nsanja, PE150

Mtundu wa laibulale: 200-400bp ikani bisulfite mankhwala DNA laibulale

Njira yotsatirira: Paired-End 150 bp

Limbikitsani kutulutsa kwa data: 10 Gb yaiwisi data/chitsanzo

Zitsanzo Zofunika

DNA kuchuluka: ≥ 2ug

Kuchuluka kwa DNA: ≥ 20ng/μl.

Chiyero: OD260/280 = 1.8 mpaka 2.0 popanda kuwonongeka kapena kuipitsidwa kwa RNA

Kusanthula kwa Bioinformatics

流程图 羽4-03

Kuyenda kwa Ntchito Yantchito

kutumiza chitsanzo

Kupereka zitsanzo

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito

  • Zam'mbuyo:
  • Ena:

    • a.Ziwerengero zofananira za ma genome

    Ziwerengero zowerengera mapu
    chithunzi3

    Sziwerengero zakuya kwakuya ndi kufalikira kwa C site

    chithunzi4

    Akufalitsa

    chithunzi5

    Digestion mphamvu ya chitsanzo chilichonse

    chithunzi6

    b.Kuzindikira kwa tsamba la methylation

    Mndandanda wa milingo ya methylation pa C site

    chithunzi7

    BS kutembenuka

    chithunzi8 chithunzi9

    Methylated cytosine
    Chithunzi 10

    Kugawa kwa methylation
    Chithunzi 11

    c.Mapu a Methylation

    Ginome-wide
    Chithunzi 12

    Gchinthu chimodzi

    Chithunzi 13

    d.Kuyerekeza kwa milingo ya methylation pakati pa zitsanzo
    Chithunzi 14

    PAirwise Kulumikizana kwa CG methylation
    Chithunzi 15

    PCA-CG methylation
    Chithunzi 16

    CKuchuluka kwa CG methylation
    Chithunzi 17

    d.Kusanthula kosiyana kwa methylation

    SZithunzi za DMR

    Chithunzi18

    Kugawa kwautali wa DMR
    Chithunzi19

    Hkudya map
    chithunzi20

    DKugawa kwa MR m'dera lomwe mukufuna
    Chithunzi 21

    pezani mtengo

    Lembani uthenga wanu apa ndikutumiza kwa ife

    Zogulitsamagulu

    Titumizireni uthenga wanu:

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