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BSA
Pulatifomu ya Bulked Segregant Analysis ili ndi kusanthula kwa gawo limodzi ndi kusanthula kwapamwamba kokhala ndi makonda okhazikika.BSA ndi njira yomwe imagwiritsidwa ntchito pozindikira mwachangu zolembera zamtundu wa phenotype.Kuyenda kwakukulu kwa BSA kuli ndi: 1. kusankha magulu awiri a anthu omwe ali ndi ma phenotypes otsutsana kwambiri;2. kuphatikiza DNA, RNA kapena SLAF-seq(Yopangidwa ndi Biomarker) ya anthu onse kuti apange DNA yambiri;3. Kuzindikiritsa kutsatizana kosiyana ndi ma genome kapena pakati, 4. kulosera madera olumikizidwa ndi ED ndi SNP-index algorithm;5. Kusanthula magwiridwe antchito ndi kulemeretsa ma jini m'magawo osankhidwa, ndi zina zambiri. Migodi yotsogola kwambiri mu data kuphatikiza kuwunika kwa ma genetic marker ndi kapangidwe koyambira ziliponso.
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Amplicon (16S/18S/ITS)
Pulatifomu ya Amplicon (16S/18S/ITS) imapangidwa ndi zaka zambiri zakusanthula kwama projekiti osiyanasiyana, omwe amakhala ndi kusanthula koyambira komanso kusanthula kwamunthu payekha: kusanthula kofunikira kumakhudza kusanthula kwakukulu kwa kafukufuku waposachedwa wa tizilombo tating'onoting'ono, zowunikira ndizolemera komanso zomveka, ndi zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a polojekiti;Zomwe zili pakuwunika kwamunthu ndizosiyanasiyana.Zitsanzo zitha kusankhidwa ndipo magawo atha kukhazikitsidwa mosinthika malinga ndi lipoti lowunikira komanso cholinga cha kafukufuku, kuti mukwaniritse zomwe mukufuna.Mawindo opangira makina, osavuta komanso ofulumira.
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Evolutionary Genetics
Evolutionary genetics ndi ntchito yotsatizana yomwe idapangidwa kuti ipereke kutanthauzira kwatsatanetsatane pazachisinthiko zazinthu zoperekedwa motengera kusiyanasiyana kwa majini, kuphatikiza ma SNP, InDels, SVs ndi CNVs.Lili ndi zofufuza zonse zofunika pofotokoza za kusintha kwa chisinthiko ndi maonekedwe a anthu, monga chiwerengero cha anthu, kusiyana kwa majini, maubwenzi a phylogeny, ndi zina zotero. Lilinso ndi maphunziro okhudza kutuluka kwa majini, zomwe zimapereka mphamvu kuyerekezera kukula kwa chiwerengero cha anthu, nthawi ya kusiyana.
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Kufananiza Genomics
Comparative genomics kwenikweni amatanthauza kufanizitsa ndondomeko yonse ya ma genome ndi mapangidwe a mitundu yosiyanasiyana.Lamuloli likufuna kuwulula zakusintha kwa mitundu, magwiridwe antchito a majini, njira yoyendetsera ma jini pamlingo wa genome pozindikira momwe amayenderana ndi zinthu zomwe zimasunga kapena kusiyanitsa mitundu yosiyanasiyana yamitundu.Kafukufuku wofananira wa ma genomics amaphatikizanso kusanthula m'banja la majini, kakulidwe kachisinthiko, kubwerezabwereza kwamtundu wonse, kukakamiza kosankha, ndi zina zambiri.
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Evolutionary Genetics
Pulatifomu yowunikira anthu komanso kusintha kwa majini imakhazikitsidwa potengera zomwe zachitika mu gulu la BMK R&D kwazaka zambiri.Ndi chida chosavuta kugwiritsa ntchito makamaka kwa ofufuza omwe sachita zazikulu mu bioinformatics.Pulatifomuyi imathandizira kusanthula koyambira kokhudzana ndi kusinthika kwa ma genetics kuphatikiza kupanga mitengo ya phylogenetic, kusanthula kwa kulumikizana, kuwunika kwamitundu yosiyanasiyana, kusanthula kosankha, kusanthula ubale, PCA, kusanthula kamangidwe ka anthu, ndi zina zambiri.
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Hi-C yochokera ku Genome Assembly
Hi-C ndi njira yomwe idapangidwa kuti ijambule masinthidwe a chromosome pophatikiza kuyesa kuyanjana kochokera kufupi ndi kutsata kwapamwamba.Kukula kwa kuyanjana uku kumakhulupirira kuti kumalumikizidwa moyipa ndi kutalika kwa ma chromosome.Chifukwa chake, data ya Hi-C imatha kuwongolera kusanjika, kuyitanitsa ndi kuwongolera katsatidwe kamene kamasonkhanitsidwa mumtundu wa ma genome ndikumangirira iwo pamitundu ina ya ma chromosome.Ukadaulo uwu umathandizira kuphatikiza kwa ma chromosome genome popanda mapu otengera kuchuluka kwa anthu.Genome iliyonse imafunikira Hi-C.
Pulatifomu: Illumina NovaSeq Platform / DNBSEQ
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Zomera / Zinyama za De Novo Genome Sequencing
De Novokutsatizana kumatanthauza kupanga matupi amtundu wonse wa zamoyo pogwiritsa ntchito umisiri wotsatizana, mwachitsanzo, PacBio, Nanopore, NGS, ndi zina zotero, ngati palibe jini lolozera.Kuwongolera kodabwitsa pakuwerenga kwautali waukadaulo wotsatizana wa m'badwo wachitatu kwabweretsa mwayi watsopano pakusonkhanitsa ma genome ovuta, monga omwe ali ndi heterozygosity yayikulu, chiŵerengero chachikulu cha zigawo zobwerezabwereza, ma polyploids, ndi zina zambiri. Kuthetsa zinthu zobwerezabwereza, zigawo zomwe zili ndi GC zosadziwika bwino ndi zigawo zina zovuta kwambiri.
Platform: PacBio Sequel II / Nanopore PromethION P48 / Illumina NovaSeq Platform
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Sequencing ya Human Whole Exome
Whole exome sequencing (WES) imatengedwa ngati njira yotsika mtengo yodziwira masinthidwe omwe amayambitsa matenda.Ngakhale ma exons amangotenga pafupifupi 1.7% ya genome yonse, imayimira mbiri ya mapuloteni onse omwe amagwira ntchito mwachindunji.Mu genome ya anthu, zanenedwa kuti kupitilira 85% ya masinthidwe okhudzana ndi matenda kumachitika m'chigawo cha protein coding.
BMKGENE imapereka njira zotsatirira komanso zosinthika za anthu okhala ndi njira zosiyanasiyana zojambulira ma exon zomwe zilipo kuti zikwaniritse zolinga zosiyanasiyana za kafukufuku.
Pulatifomu: Illumina NovaSeq Platform
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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
Ma genotyping apamwamba kwambiri, makamaka pa anthu ochuluka, ndi gawo lofunikira kwambiri pa maphunziro a ma genetic, omwe amapereka maziko a chibadwa cha kutulukira kwa majini, kusanthula kwachisinthiko, ndi zina zotero. ) imayambitsidwa pofuna kuchepetsa mtengo wotsatizanatsatizana pachitsanzo chilichonse, komanso kukhalabe ndi luso lokwanira pakutulukira kwa chibadwa.Izi nthawi zambiri zimatheka pochotsa chidutswa choletsa mkati mwa kukula kwake, chomwe chimatchedwa laibulale yoyimira (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) ndi njira yodzipangira yokha ya SNP genotyping yokhala ndi kapena popanda genome.
Pulatifomu: Illumina NovaSeq Platform -
Illumina ndi BGI
Tekinoloje yotsatizana ya Illumina, yozikidwa pa Sequencing by Synthesis (SBS), ndiukadaulo wapadziko lonse wa NGS, womwe umapangitsa kuti pakhale 90% yazotsatizana zapadziko lonse lapansi.Mfundo ya SBS imaphatikizapo kujambula zoziziritsa zotchedwa fulorosenti zotchedwa reversible terminators pamene dNTP iliyonse imawonjezedwa, ndipo kenako imadulidwa kuti ilole kuphatikizidwa kwa maziko otsatirawa.Ndi ma dNTP onse anayi osinthika omangika otsekera omwe amapezeka mumayendedwe aliwonse, mpikisano wachilengedwe umachepetsa kukondera.Ukadaulo wosunthikawu umathandizira malaibulale owerengeka okha komanso ophatikizidwa awiriawiri, popereka ma genomic applications.Illumina sequencing imatengera luso lapamwamba kwambiri komanso kulondola kwake, imayiyika ngati mwala wapangodya pa kafukufuku wa genomics, kupatsa mphamvu asayansi kuti avumbulutse zovuta za ma genomes mwatsatanetsatane komanso mosayerekezeka.
DNBSEQ, yopangidwa ndi BGI, ndiukadaulo winanso waukadaulo wa NGS womwe wakwanitsa kutsitsa mtengo wotsatizana ndikuwonjezera zotuluka.Kukonzekera kwa malaibulale a DNBSEQ kumaphatikizapo kugawikana kwa DNA, kukonzekera ssDNA ndi kukweza kozungulira kuti mupeze ma nanoballs a DNA (DNB).Izi zimayikidwa pamalo olimba ndikutsatiridwa ndi combinatorial Probe-Anchor Synthesis (cPAS).
Ntchito yathu yotsatirira laibulale yomwe idapangidwa kale imathandizira makasitomala kukonzekera malaibulale awo otsatizana kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, pakati pa ena).Pambuyo pake, malaibulalewa atha kutumizidwa kumalo athu otsatirira kuti azitha kuyang'anira bwino komanso kutsatizana pamapulatifomu a Illumina kapena BGI.
