-
Kutsata kwa Metatranscriptome
Kutsatizana kwa metatranscriptome kumazindikiritsa ma jini a ma microbes (onse a eukaryotes ndi ma prokaryotes) mkati mwa chilengedwe (monga nthaka, madzi, nyanja, ndowe, ndi m'matumbo). za mitundu, kusanthula magwiridwe antchito amitundu yosiyanasiyana, ndi zina zambiri.
Pulatifomu: Illumina NovaSeq Platform
-
Fungal Genome
Biomarker Technologies imapereka kafukufuku wa ma genome, genome yabwino komanso genome yokwanira ya fangasi kutengera cholinga cha kafukufuku wina.Kutsatizana kwa ma genome, kusonkhanitsa ndi kutanthauzira kogwira ntchito kungathe kupezedwa mwa kuphatikiza kutsatizana kwa m'badwo Wotsatira + Kutsatizana kwa mbadwo wachitatu kuti mukwaniritse msonkhano wapamwamba kwambiri wa genome.Ukadaulo wa Hi-C utha kugwiritsidwanso ntchito kuti uthandizire kuphatikiza ma genome pamlingo wa chromosome.
nsanja:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
-
Zida Zazida
BMKCloud ndi nsanja yotsogola ya bioinformatic yopereka njira imodzi yokha yamapulogalamu a genomic, omwe amadaliridwa kwambiri ndi ofufuza m'mabwalo osiyanasiyana kuphatikiza azachipatala, zaulimi, zachilengedwe, ndi zina. BMKCloud yadzipereka kupereka ntchito zophatikizika, zodalirika komanso zogwira mtima kuphatikiza nsanja ndi zida zowunikira bioinformatics. . kusanthula, etc.
-
Mabakiteriya Onse Genome
Biomarker Technologies imapereka ntchito zotsatizana popanga ma genome athunthu a mabakiteriya okhala ndi zero gap.Kuyenda kwakukulu kwa mabakiteriya omanga ma genome kumaphatikizapo kutsatizana kwa m'badwo wachitatu, kusonkhanitsa, kafotokozedwe kantchito komanso kusanthula kwapamwamba kwa bioinformatics kukwaniritsa zolinga za kafukufuku.Kufotokozera mwatsatanetsatane za mabakiteriya genome kumapatsa mphamvu kuwulula njira zofunika kwambiri zomwe zimathandizira kuti chilengedwe chikhalepo, chomwe chingaperekenso chidziwitso chofunikira pakufufuza kwa ma genomic mu mitundu yapamwamba ya eukaryotic.
nsanja:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
-
RNA yaying'ono
Ma RNA ang'onoang'ono ndi mtundu wa RNA yayifupi yosalemba yomwe ili ndi kutalika kwa 18-30 nt, kuphatikiza miRNA, siRNA ndi piRNA.Ma RNA ang'onoang'ono awa akhala akufotokozedwa mochuluka kuti akugwira nawo ntchito zosiyanasiyana zamoyo monga kuwonongeka kwa mRNA, kulepheretsa kumasulira, kupanga heterochromatin, ndi zina zotero. SmallRNA yotsatizana kufufuza kwagwiritsidwa ntchito kwambiri mu maphunziro okhudza chitukuko cha zinyama / zomera, matenda, kachilombo, ndi zina zotero. Sequencing kusanthula nsanja imakhala ndi kusanthula kokhazikika komanso migodi yapa data yapamwamba.Pamaziko a data ya RNA-seq, kusanthula kokhazikika kumatha kukwaniritsa chizindikiritso ndi kulosera kwa miRNA, kulosera kwamtundu wa miRNA, kusanthula ndi kusanthula mawu.Kusanthula kwapamwamba kumathandizira kusaka ndi kutulutsa kwa miRNA mwamakonda, kupanga zojambula za Venn, miRNA ndikumanga ma network a chandamale.
-
NGS-WGS (Illumina/BGI)
NGS-WGS ndi nsanja yonse yowunikiranso ma genome, yomwe imapangidwa potengera luso lolemera mu Biomarker Technologies.Pulatifomu yosavuta kugwiritsa ntchito iyi imalola kutumizira mwachangu kusanthula kophatikizika kwa kachitidwe pongoyika magawo angapo oyambira, omwe amakwanira ma data a DNA opangidwa kuchokera ku nsanja ya Illumina ndi nsanja yotsatirira ya BGI.Pulatifomuyi imayikidwa pa seva yapakompyuta yogwira ntchito kwambiri, yomwe imapatsa mphamvu kusanthula kwachangu kwa data munthawi yochepa kwambiri.Kutsitsa kwamunthu payekha kumapezeka potengera kusanthula kokhazikika, kuphatikiza funso la gene, PCR primer design, etc.
-
mRNA(Reference)
Transcriptome ndi mgwirizano pakati pa chidziwitso cha ma genetic ndi proteome ya biological function.Kuwongolera mulingo wa transcriptal ndi njira yofunikira kwambiri komanso yophunziridwa kwambiri pazamoyo.Kutsatizana kwa Transcriptome kumatha kutsatizana zolembedwazo nthawi iliyonse kapena munthawi iliyonse, ndi chigamulo cholondola ku nucleotide imodzi. Imatha kuwonetsa mozama kuchuluka kwa kulembedwa kwa jini, kuzindikira ndikuwerengera zolembedwa zosawerengeka komanso zachilendo, ndikupereka zidziwitso zamapangidwe a zitsanzo zolembedwa zenizeni.
Pakalipano, teknoloji yotsatizana ya transcriptome yakhala ikugwiritsidwa ntchito kwambiri muzofukufuku, zamankhwala ndi zina zofufuza, kuphatikizapo malamulo a chitukuko cha zinyama ndi zomera, kusintha kwa chilengedwe, kugwirizana kwa chitetezo cha mthupi, kutanthauzira kwa jini, kusinthika kwamtundu wamitundu ndi chotupa ndi matenda a majini.
-
Metagenomics (NGS)
Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya shotgun metagenomic pazaka zazaka zambiri.Zili ndi kayendetsedwe ka ntchito kophatikizana komwe kamakhala ndi kusanthula kosiyanasiyana komwe kumafunikira kwambiri kuphatikiza kusanthula kwa data, maphunziro amtundu wamitundu, maphunziro amtundu wa jini, binning ya metagenome, ndi zina zambiri. , kuyika magawo, kupanga kwamunthu payekha, ndi zina.
-
LncRNA
Ma RNA aatali osalemba zilembo (lncRNA) ndi mtundu wa zolembedwa zotalika kuposa 200 nt, zomwe sizitha kulemba ma protein.Umboni wochuluka umasonyeza kuti ma lncRNA ambiri amatha kugwira ntchito.Ukadaulo wotsogola wotsogola kwambiri ndi zida zowunikira za bioinformatic zimatipatsa mphamvu kuti tiwulule masanjidwe a lncRNA ndikuyika zidziwitso moyenera komanso kutitsogolera kuti tipeze ma lncRNA okhala ndi ntchito zowongolera.BMKCloud imanyadira kupatsa makasitomala athu lncRNA nsanja yowunikira kuti akwaniritse kusanthula kwachangu, kodalirika komanso kosinthika kwa lncRNA.
-
GWAS
Genome-wide association study (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imakhudzana ndi makhalidwe enaake (phenotype).Kafukufuku wa GWA amafufuza zolembera zamtundu wamtundu wonse wa anthu ambiri ndikulosera mayanjano a genotype-phenotype posanthula kuchuluka kwa anthu.Kubwereza kwamtundu wonse kumatha kupeza mitundu yonse ya ma genetic.Kuphatikizana ndi deta ya phenotypic, GWAS ikhoza kukonzedwa kuti izindikire ma SNP okhudzana ndi phenotype, QTLs ndi majini osankhidwa, omwe amathandizira kwambiri kuswana nyama / zomera zamakono.SLAF ndi njira yodzipangira yokha yosavuta yotsatirira ma genome, yomwe imapeza zolembera zamtundu uliwonse, SNP.Ma SNP awa, monga ma genetic markers, amatha kukonzedwa kuti agwirizane ndi zomwe akutsata.Ndi njira yotsika mtengo pozindikira zovuta zomwe zimagwirizanitsidwa ndi kusiyanasiyana kwa ma genetic.
-
Nanopore Full-length transcriptomics
Ma isoform ovuta komanso osinthika m'zamoyo ndi njira zofunika kwambiri zoyendetsera ma jini komanso kusiyanasiyana kwa mapuloteni.Kuzindikiritsa kolondola kwa zolembedwa ndi maziko owerengera mozama machitidwe owongolera ma jini.Nanopore sequencing platform yabweretsa bwino transcriptomic study ku isoform-level.Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya RNA-Seq yopangidwa pa nsanja ya Nanopore pamaziko a genome, yomwe imakwaniritsa kusanthula kwamtundu komanso kuchuluka kwamitundu yonse komanso mulingo wa zolembedwa.
-
kuzungulira-RNA
RNA yozungulira (circRNA) ndi mtundu wa RNA yosalemba, yomwe posachedwapa imapezeka kuti ikugwira ntchito yofunika kwambiri pamagulu olamulira omwe akukhudzidwa ndi chitukuko, kukana chilengedwe, ndi zina zotero. Zosiyana ndi mamolekyu a RNA, mwachitsanzo mRNA, lncRNA, 3' ndi 5'. malekezero a circRNA amalumikizidwa palimodzi kuti apange mawonekedwe ozungulira, omwe amawapulumutsa ku chimbudzi cha exonuclease ndipo amakhala okhazikika kuposa RNA yambiri.CircRNA yapezeka kuti ili ndi ntchito zosiyanasiyana pakuwongolera ma jini.CircRNA imatha kuchita ngati ceRNA, yomwe imamanga miRNA mopikisana, yotchedwa miRNA siponji.CircRNA sequencing analysis platform imapatsa mphamvu circRNA kamangidwe ndi kusanthula mawu, kulosera chandamale ndi kusanthula pamodzi ndi mitundu ina ya mamolekyu a RNA.
