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Metagenomic Sequencing -NGS
Metagenome imatanthawuza kusonkhanitsa kwa chibadwa chamagulu osakanikirana a zamoyo monga chilengedwe, metagenome yaumunthu, ndi zina zotero. Lili ndi majeremusi a tizilombo tomwe timalima komanso tosalimidwa.Metagenomic sequencing ndi chida cha mamolekyu chomwe chimagwiritsidwa ntchito posanthula zinthu zosakanizika za ma genomic zotengedwa ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chambiri pamitundu yosiyanasiyana komanso kuchuluka kwake, kuchuluka kwa anthu, ubale wa phylogenetic, majini ogwira ntchito komanso maukonde olumikizana ndi chilengedwe.
nsanja:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics ndi chida cha molekyulu chomwe chimagwiritsidwa ntchito pofufuza zinthu zosakanikirana zamtundu wamtundu zomwe zimatengedwa kuchokera ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chambiri pamitundu yosiyanasiyana ya zamoyo ndi kuchuluka kwake, kuchuluka kwa anthu, ubale wa phylogenetic, majini ogwirira ntchito ndi maukonde ogwirizana ndi zinthu zachilengedwe, etc. Mapulatifomu a Nanopore adayambitsa posachedwa ku maphunziro a metagenomic.Kuchita kwake kwabwino pakuwerenga kwanthawi yayitali kunapititsa patsogolo kusanthula kwa metagenomic, makamaka msonkhano wa metagenome.Kutengera ubwino wowerengera kutalika, kafukufuku wa Nanopore-based metagenomic amatha kukwaniritsa msonkhano wopitilirabe poyerekeza ndi kuwombera mfuti.Zasindikizidwa kuti metagenomics yochokera ku Nanopore idapanga bwino ma genome athunthu komanso otsekedwa a bakiteriya kuchokera ku ma microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
nsanja:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit pa 16S ndi 18S rRNA yomwe ili ndi zigawo zonse zotetezedwa kwambiri komanso zosinthika kwambiri ndi chala chabwino kwambiri cha molekyulu yozindikiritsa zamoyo za prokaryotic ndi eukaryotic.Kutengera mwayi wotsatizana, ma amplicon awa amatha kuyang'ana pazigawo zosungidwa ndipo madera osinthika amatha kudziwika bwino kuti tizindikire tizilombo toyambitsa matenda zomwe zimathandizira pamaphunziro okhudza kusanthula kwamitundu yosiyanasiyana, taxonomy, phylogeny, ndi zina. ) kutsatizana kwa nsanja ya PacBio kumathandizira kupeza zowerengera zolondola kwambiri, zomwe zimatha kuphimba ma amplicons aatali (pafupifupi 1.5 KB).Kuwona kokulirapo kwa ma genetic kumathandizira kwambiri kusintha kwa mitundu ya mabakiteriya kapena bowa.
nsanja:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing ikufuna kuwulula kuchuluka kwa phylogeny, taxonomy, ndi mitundu yamitundu m'magulu ang'onoang'ono pofufuza zinthu za PCR za zolembera zanyumba zomwe zili ndi magawo omwe amakambirana kwambiri komanso osasinthika.Kuyambitsidwa kwa zala zabwino kwambiri zamamolekyulu olembedwa ndi Woeses et al, (1977) kumapereka mphamvu zodzipatula za microbiome.Kutsatizana kwa 16S (mabakiteriya), 18S (bowa) ndi Internal transcribed spacer(ITS, bowa) amalola kuzindikirika kwa mitundu yambirimbiri komanso mitundu yosowa komanso yosadziwika.Tekinoloje iyi yakhala chida chogwiritsidwa ntchito kwambiri komanso chachikulu pakuzindikiritsa mitundu yosiyanasiyana ya tizilombo m'malo osiyanasiyana, monga pakamwa pamunthu, matumbo, ndowe, ndi zina zambiri.
nsanja:Illumina NovaSeq Platform
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Mabakiteriya ndi fungal Yense ya Genome Yotsatizana
Kutsatizananso kwa ma genome ndi mabakiteriya ndi mafangasi ndi chida chofunikira kwambiri pomaliza ma genome a mabakiteriya odziwika ndi mafangasi, komanso kuyerekeza ma genome angapo kapena kujambula ma genome a zamoyo zatsopano.Ndikofunikira kwambiri kutsata ma genome athunthu a bakiteriya ndi bowa kuti apange ma genomes olondola, kupanga zozindikiritsa tizilombo ndi maphunziro ena ofananiza ma genome.
Pulatifomu: Illumina NovaSeq Platform
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Kutsata kwa Metatranscriptome
Kutsatizana kwa metatranscriptome kumazindikiritsa ma jini a ma microbes (onse a eukaryotes ndi ma prokaryotes) mkati mwa chilengedwe (monga nthaka, madzi, nyanja, ndowe, ndi m'matumbo). za mitundu, kusanthula magwiridwe antchito amitundu yosiyanasiyana, ndi zina zambiri.
Pulatifomu: Illumina NovaSeq Platform
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Fungal Genome
Biomarker Technologies imapereka kafukufuku wa ma genome, genome yabwino komanso genome yokwanira ya fangasi kutengera cholinga cha kafukufuku wina.Kutsatizana kwa ma genome, kusonkhanitsa ndi kutanthauzira kogwira ntchito kungathe kupezedwa mwa kuphatikiza kutsatizana kwa m'badwo Wotsatira + Kutsatizana kwa mbadwo wachitatu kuti mukwaniritse msonkhano wapamwamba kwambiri wa genome.Ukadaulo wa Hi-C utha kugwiritsidwanso ntchito kuti uthandizire kuphatikiza ma genome pamlingo wa chromosome.
nsanja:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq Platform
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Mabakiteriya Onse Genome
Biomarker Technologies imapereka ntchito zotsatizana popanga ma genome athunthu a mabakiteriya okhala ndi zero gap.Kuyenda kwakukulu kwa mabakiteriya omanga ma genome kumaphatikizapo kutsatizana kwa m'badwo wachitatu, kusonkhanitsa, kafotokozedwe kantchito komanso kusanthula kwapamwamba kwa bioinformatics kukwaniritsa zolinga za kafukufuku.Kufotokozera mwatsatanetsatane za mabakiteriya genome kumapatsa mphamvu kuwulula njira zofunika kwambiri zomwe zimathandizira kuti chilengedwe chikhalepo, chomwe chingaperekenso chidziwitso chofunikira pakufufuza kwa ma genomic mu mitundu yapamwamba ya eukaryotic.
nsanja:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
