-
Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq imapereka mbiri ya genome-wide ya DNA zomwe zikufuna kusintha histone, transcript factor, ndi mapuloteni ena okhudzana ndi DNA.Imaphatikiza kusankha kwa chromatin immuno-precipitation (ChIP) kuti ipezenso ma protein-DNA complexes, ndi mphamvu ya m'badwo wotsatira (NGS) pakutsatizana kwapamwamba kwa DNA yobwezeretsedwa.Kuphatikiza apo, chifukwa ma protein-DNA complexes amatengedwa kuchokera ku maselo amoyo, malo omangira amatha kufananizidwa mumitundu yosiyanasiyana ya maselo ndi minofu, kapena pansi pamikhalidwe yosiyana.Mapulogalamuwa amachokera ku malamulo olembedwa mpaka njira zachitukuko kupita ku njira za matenda ndi kupitirira.
Pulatifomu: Illumina NovaSeq Platform
-
Kutsatizana konse kwa genome bisulfite
DNA methylation pamalo achisanu mu cytosine (5-mC) imakhudza kwambiri mafotokozedwe a majini ndi zochitika zama cell.Ma methylation osadziwika bwino amalumikizidwa ndi mikhalidwe ndi matenda angapo, monga khansa.WGBS yakhala muyezo wagolide wophunzirira genome-wide methylation pa single base resolution.
Pulatifomu: Illumina NovaSeq Platform
-
Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
ATAC-seq ndi njira yotsatirira kwambiri yowunikira kupezeka kwa chromatin ya genome, yomwe ndiyofunikira pakuwongolera ma epigenetic padziko lonse lapansi pakuwonetsa ma jini.Ma adapter otsatizana amalowetsedwa m'magawo otseguka a chromatin ndi hyperactive Tn5 transposase.Pambuyo pakukulitsa kwa PCR, laibulale yotsatizana imapangidwa.Magawo onse a chromatin otseguka amatha kupezeka pansi pa nthawi yeniyeni ya nthawi, osati kungokhala ndi malo omangirira a chinthu cholembera, kapena dera linalake losinthidwa la histone.
-
Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)
Kafukufuku wa DNA methylation nthawi zonse wakhala mutu wovuta kwambiri pa kafukufuku wa matenda, ndipo umagwirizana kwambiri ndi maonekedwe a jini ndi makhalidwe a pheno-typic.RRBS ndi njira yolondola, yothandiza komanso yachuma pa kafukufuku wa DNA methylation.Kulemeretsa kwa zigawo za zilumba za CpG ndi enzymatic cleavage (Msp I), kuphatikizidwa ndi kutsatizana kwa Bisulfite, kumapereka kuzindikira kwakukulu kwa DNA methylation.
Pulatifomu: Illumina NovaSeq Platform
