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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Pulatifomu ya Amplicon (16S/18S/ITS) imapangidwa ndi zaka zambiri zakusanthula kwama projekiti osiyanasiyana, omwe amakhala ndi kusanthula koyambira komanso kusanthula kwamunthu payekha: kusanthula kofunikira kumakhudza kusanthula kwakukulu kwa kafukufuku waposachedwa wa tizilombo tating'onoting'ono, zowunikira ndizolemera komanso zomveka, ndi zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a polojekiti;Zomwe zili pakuwunika kwamunthu ndizosiyanasiyana.Zitsanzo zitha kusankhidwa ndipo magawo atha kukhazikitsidwa mosinthika malinga ndi lipoti lowunikira komanso cholinga cha kafukufuku, kuti mukwaniritse zomwe mukufuna.Mawindo opangira makina, osavuta komanso ofulumira.
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PacBio-Full-length Transcriptome (Non-Reference)
Kutenga data ya Pacific Bioscience (PacBio) Isoform yotsatirira monga zolowetsa, Pulogalamuyi imatha kuzindikira masanjidwe aatali athunthu (popanda kuphatikiza).Popanga mapu a mautali athunthu motsutsana ndi ma genome, zolembedwa zimatha kukongoletsedwa ndi majini odziwika, zolembedwa, zigawo zolembera, ndi zina zotere. Pankhaniyi, kuzindikirika kolondola kwa mapangidwe a mRNA, monga kuphatikizika kwina, ndi zina, zitha kukwaniritsidwa.Kusanthula kophatikizana ndi data ya NGS transcriptome sequencing kumathandizira kutanthauzira momveka bwino komanso kuchulukitsidwa kolondola kwambiri pofotokozera pamlingo wa transcript, zomwe zimapindulitsa kwambiri kufotokozera kosiyanasiyana komanso kusanthula magwiridwe antchito.
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Zida Zazida
BMKCloud ndi nsanja yotsogola ya bioinformatic yopereka njira imodzi yokha yamapulogalamu a genomic, omwe amadaliridwa kwambiri ndi ofufuza m'mabwalo osiyanasiyana kuphatikiza azachipatala, zaulimi, zachilengedwe, ndi zina. BMKCloud yadzipereka kupereka ntchito zophatikizika, zodalirika komanso zogwira mtima kuphatikiza nsanja ndi zida zowunikira bioinformatics. . kusanthula, etc.
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RNA yaying'ono
Ma RNA ang'onoang'ono ndi mtundu wa RNA yayifupi yosalemba yomwe ili ndi kutalika kwa 18-30 nt, kuphatikiza miRNA, siRNA ndi piRNA.Ma RNA ang'onoang'ono awa akhala akufotokozedwa mochuluka kuti akugwira nawo ntchito zosiyanasiyana zamoyo monga kuwonongeka kwa mRNA, kulepheretsa kumasulira, kupanga heterochromatin, ndi zina zotero. SmallRNA yotsatizana kufufuza kwagwiritsidwa ntchito kwambiri mu maphunziro okhudza chitukuko cha zinyama / zomera, matenda, kachilombo, ndi zina zotero. Sequencing kusanthula nsanja imakhala ndi kusanthula kokhazikika komanso migodi yapa data yapamwamba.Pamaziko a data ya RNA-seq, kusanthula kokhazikika kumatha kukwaniritsa chizindikiritso ndi kulosera kwa miRNA, kulosera kwamtundu wa miRNA, kusanthula ndi kusanthula mawu.Kusanthula kwapamwamba kumathandizira kusaka ndi kutulutsa kwa miRNA mwamakonda, kupanga zojambula za Venn, miRNA ndikumanga ma network a chandamale.
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NGS-WGS (Illumina/BGI)
NGS-WGS ndi nsanja yonse yowunikiranso ma genome, yomwe imapangidwa potengera luso lolemera mu Biomarker Technologies.Pulatifomu yosavuta kugwiritsa ntchito iyi imalola kutumizira mwachangu kusanthula kophatikizika kwa kachitidwe pongoyika magawo angapo oyambira, omwe amakwanira ma data a DNA opangidwa kuchokera ku nsanja ya Illumina ndi nsanja yotsatirira ya BGI.Pulatifomuyi imayikidwa pa seva yapakompyuta yogwira ntchito kwambiri, yomwe imapatsa mphamvu kusanthula kwachangu kwa data munthawi yochepa kwambiri.Kutsitsa kwamunthu payekha kumapezeka potengera kusanthula kokhazikika, kuphatikiza funso la gene, PCR primer design, etc.
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mRNA(Reference)
Transcriptome ndi mgwirizano pakati pa chidziwitso cha ma genetic ndi proteome ya biological function.Kuwongolera mulingo wa transcriptal ndi njira yofunikira kwambiri komanso yophunziridwa kwambiri pazamoyo.Kutsatizana kwa Transcriptome kumatha kutsatizana zolembedwazo nthawi iliyonse kapena munthawi iliyonse, ndi chigamulo cholondola ku nucleotide imodzi. Imatha kuwonetsa mozama kuchuluka kwa kulembedwa kwa jini, kuzindikira ndikuwerengera zolembedwa zosawerengeka komanso zachilendo, ndikupereka zidziwitso zamapangidwe a zitsanzo zolembedwa zenizeni.
Pakalipano, teknoloji yotsatizana ya transcriptome yakhala ikugwiritsidwa ntchito kwambiri muzofukufuku, zamankhwala ndi zina zofufuza, kuphatikizapo malamulo a chitukuko cha zinyama ndi zomera, kusintha kwa chilengedwe, kugwirizana kwa chitetezo cha mthupi, kutanthauzira kwa jini, kusinthika kwamtundu wamitundu ndi chotupa ndi matenda a majini.
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Metagenomics (NGS)
Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya shotgun metagenomic pazaka zazaka zambiri.Zili ndi kayendetsedwe ka ntchito kophatikizana komwe kamakhala ndi kusanthula kosiyanasiyana komwe kumafunikira kwambiri kuphatikiza kusanthula kwa data, maphunziro amtundu wamitundu, maphunziro amtundu wa jini, binning ya metagenome, ndi zina zambiri. , kuyika magawo, kupanga kwamunthu payekha, ndi zina.
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LncRNA
Ma RNA aatali osalemba zilembo (lncRNA) ndi mtundu wa zolembedwa zotalika kuposa 200 nt, zomwe sizitha kulemba ma protein.Umboni wochuluka umasonyeza kuti ma lncRNA ambiri amatha kugwira ntchito.Ukadaulo wotsogola wotsogola kwambiri ndi zida zowunikira za bioinformatic zimatipatsa mphamvu kuti tiwulule masanjidwe a lncRNA ndikuyika zidziwitso moyenera komanso kutitsogolera kuti tipeze ma lncRNA okhala ndi ntchito zowongolera.BMKCloud imanyadira kupatsa makasitomala athu lncRNA nsanja yowunikira kuti akwaniritse kusanthula kwachangu, kodalirika komanso kosinthika kwa lncRNA.
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GWAS
Genome-wide association study (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imakhudzana ndi makhalidwe enaake (phenotype).Kafukufuku wa GWA amafufuza zolembera zamtundu wamtundu wonse wa anthu ambiri ndikulosera mayanjano a genotype-phenotype posanthula kuchuluka kwa anthu.Kubwereza kwamtundu wonse kumatha kupeza mitundu yonse ya ma genetic.Kuphatikizana ndi deta ya phenotypic, GWAS ikhoza kukonzedwa kuti izindikire ma SNP okhudzana ndi phenotype, QTLs ndi majini osankhidwa, omwe amathandizira kwambiri kuswana nyama / zomera zamakono.SLAF ndi njira yodzipangira yokha yosavuta yotsatirira ma genome, yomwe imapeza zolembera zamtundu uliwonse, SNP.Ma SNP awa, monga ma genetic markers, amatha kukonzedwa kuti agwirizane ndi zomwe akutsata.Ndi njira yotsika mtengo pozindikira zovuta zomwe zimagwirizanitsidwa ndi kusiyanasiyana kwa ma genetic.
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Nanopore Full-length transcriptomics
Ma isoform ovuta komanso osinthika m'zamoyo ndi njira zofunika kwambiri zoyendetsera ma jini komanso kusiyanasiyana kwa mapuloteni.Kuzindikiritsa kolondola kwa zolembedwa ndi maziko owerengera mozama machitidwe owongolera ma jini.Nanopore sequencing platform yabweretsa bwino transcriptomic study ku isoform-level.Pulatifomu yowunikirayi idapangidwa kuti iwunikenso data ya RNA-Seq yopangidwa pa nsanja ya Nanopore pamaziko a genome, yomwe imakwaniritsa kusanthula kwamtundu komanso kuchuluka kwamitundu yonse komanso mulingo wa zolembedwa.
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kuzungulira-RNA
RNA yozungulira (circRNA) ndi mtundu wa RNA yosalemba, yomwe posachedwapa imapezeka kuti ikugwira ntchito yofunika kwambiri pamagulu olamulira omwe akukhudzidwa ndi chitukuko, kukana chilengedwe, ndi zina zotero. Zosiyana ndi mamolekyu a RNA, mwachitsanzo mRNA, lncRNA, 3' ndi 5'. malekezero a circRNA amalumikizidwa palimodzi kuti apange mawonekedwe ozungulira, omwe amawapulumutsa ku chimbudzi cha exonuclease ndipo amakhala okhazikika kuposa RNA yambiri.CircRNA yapezeka kuti ili ndi ntchito zosiyanasiyana pakuwongolera ma jini.CircRNA imatha kuchita ngati ceRNA, yomwe imamanga miRNA mopikisana, yotchedwa miRNA siponji.CircRNA sequencing analysis platform imapatsa mphamvu circRNA kamangidwe ndi kusanthula mawu, kulosera chandamale ndi kusanthula pamodzi ndi mitundu ina ya mamolekyu a RNA.
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BSA
Pulatifomu ya Bulked Segregant Analysis ili ndi kusanthula kwa gawo limodzi ndi kusanthula kwapamwamba kokhala ndi makonda okhazikika.BSA ndi njira yomwe imagwiritsidwa ntchito pozindikira mwachangu zolembera zamtundu wa phenotype.Kuyenda kwakukulu kwa BSA kuli ndi: 1. kusankha magulu awiri a anthu omwe ali ndi ma phenotypes otsutsana kwambiri;2. kuphatikiza DNA, RNA kapena SLAF-seq(Yopangidwa ndi Biomarker) ya anthu onse kuti apange DNA yambiri;3. Kuzindikiritsa kutsatizana kosiyana ndi ma genome kapena pakati, 4. kulosera madera olumikizidwa ndi ED ndi SNP-index algorithm;5. Kusanthula magwiridwe antchito ndi kulemeretsa ma jini m'magawo osankhidwa, ndi zina zambiri. Migodi yotsogola kwambiri mu data kuphatikiza kuwunika kwa ma genetic marker ndi kapangidwe koyambira ziliponso.
