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KUSINTHA KWA GENOME

Genome De Novo
Msonkhano | Kutsimikiza pakugonana Ntchito zonse zotsatizana ndi ntchito zina za bioinformatic zidaperekedwa ndi Biomarker Techonologies.

Ndemanga

Mitundu yodziwika bwino ya zinjoka za m’nyanjayi imaphatikizapo zomangira zooneka ngati masamba, kukamwa kopanda mano, ndi mimba yamphongo yoikidwira mazira pa “chigamba” chotseguka.We de novo–kutsatizana ma genome aamuna ndi aakazi a common seadragon (Phyllopteryx taeniolatus) ndi mitundu yofananira yake, alligator pipefish (Syngnathoides biaculeatus).Mbiri yolembedwa kuchokera kuzinthu zachilendo, zomangika ngati masamba, zikuwonetsa kuti mitundu yambiri ya majini yomwe imakhudzidwa kwambiri ndi chitukuko adasankhidwa pamodzi komanso kulemeretsa zolemba zomwe zingathe kukonzanso minofu ndi majini oteteza chitetezo chathupi.Zosintha za zebrafish za scpp5, zomwe zimatayika mu ma syngnathids onse, zidapezeka kuti zilibe kapena zili ndi mano opunduka a pharyngeal, kuchirikiza lingaliro loti kutayika kwa scpp5 kwathandizira kutha kwa mano mu syngnathids.Gulu lodziwikiratu lokhala ndi jini ya amhr2y yamwamuna yogawana ndi wamba seadragon ndi alligator pipefish idadziwika. 

Zida ndi njira:

Zipangizo

Fchinjoka chathu wamba (P. taeniolatus) ndi nsomba ziwiri za alligator (S. biaculeatus) zitsanzo.Seadragon awiri wamba (m'modzi wamwamuna ndi wamkazi) ndi alligator pipefish (m'modzi wamwamuna ndi wamkazi) adagwiritsidwa ntchito potsata ma genome onse, ndipo amuna ena awiri aamuna amtundu wa seadragon adagwiritsidwa ntchito pofananiza ma genome.

Genome Sequencing strategy

270/350 bp library (Hiseq 2500) + Pacbio 20Kb (Sequel) kapena Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .sequencing, ndi amuna ena awiri aamuna wamba seadragon anthu anagwiritsiridwa ntchito kwa genome requencing.

Kutsata kwa Transcriptome

Fkapena seadragon wamba, ubongo, diso, gill, chiwindi, mtima, impso, mesentery, matumbo, minofu, zipsepse, khungu, zokhala ngati masamba, testis, ndi ovary zinasonkhanitsidwa.

Njira ya msonkhano wa Genome de novo

Canu (Kuwongolera) + WTDBG2 (Assembly) + Pilon(Polish) + Lachesis (Hi-C).

Zotsatira Zazikulu

Chithunzi-1.-Mfungulo-za-wamba-seadragon-P.-taeniolatus-ndi-the-alligator-pipefish-S

Chithunzi 1. Zinthu zazikuluzikulu za seadragon wamba (P. taeniolatus) ndi alligator pipefish (S. biaculeatus) ndi malo awo a phylogenetic.

Chithunzi-2.-Morphology-ndi-genetic-mapangidwe-a-tsamba-ngati-zowonjezera-mu-wamba-seadragon-P

Chithunzi 2. Mapangidwe a ma morphology ndi ma genetic a appendages ngati masamba a common seadragon (P. taeniolatus).

Chithunzi-3.-Putative-sex-determination-gene-in-the-common-seadragon-P.-taeniolatus-and-the-alligator-pipefish-S.-biaculeatus

Chithunzi 3. Chinjoka cham'madzi cha common seadragon (P. taeniolatus) ndi alligator pipefish (S. biaculeatus).

Chithunzi-4

Chithunzi 4. Pharyngeal dzino phenotypes mu zebrafish scpp5 homozygous mutants.


Nthawi yotumiza: Jan-08-2022

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