Mu 2021, BMKGENE umboni 31 De novo genome kafukufuku bwinobwino lofalitsidwa mu mkulu-mphamvu magazini ndi okwana zotsatira zinthu pa 320. 15 wa nkhani anali co-olemba 4 a iwo anali co-olemba monga oyamba olemba ndi BMKGENE.
Chaka cha 2022 chitangoyamba kumene, pakhala kale zolemba ziwiri zofufuza zomwe zidasindikizidwa mu nyuzipepala ya "Natural Genetics" ndi "Molecular Plant" motsatana.Ndiwo, "Ma haplotypes awiri amtundu wa heterozygous lychee genome akuwonetsa zochitika zoweta zodziyimira pawokha zamitundu yoyambilira komanso yokhwima mochedwa" (kafukufuku wa Lychee genome, wochitidwa ndi College of Horticulture, South China Agricultural university, ndi othandizira asayansi, lofalitsidwa pa Natural Genetics. ), ndi "Kutsatizana kwa ma genome a mitundu isanu ya Sitopsis ya Aegilops ndi chiyambi cha tirigu wa polyploid B-subgenome" (Five Sitopsis mitundu genome, yochitidwa ndi gulu lofufuza la Professor Bao Liu la Northeast Normal University.).Tikambirananso nkhani ziwirizi ndikugawana ndi owerenga athu.
Tsopano, tiyeni tiwone zolemba zabwino kwambiri zofufuzira zomwe zidasindikizidwa mu 2021 zolembedwa ndi BMK ndi malo athu ogwirira ntchito.
Zomera zamtundu - Zowoneka bwino pamitundu yambiri.
1. Gulu lapamwamba kwambiri la ma genome limawunikira mawonekedwe a rye genomic ndi majini ofunikira pazaulimi.
Malo Othandizira: Henan Agricultural University
Journal: Natural Genetics
Zotsatira Zake: 38.31
Mu ntchitoyi, mtundu wa Weining rye, mtundu wapamwamba kwambiri wa rye waku China, unatsatiridwa.Ma contigs ophatikizidwa (7.74 Gb) anali 98.47% ya kukula kwa genome (7.86 Gb), ndi 93.67% ya contigs (7.25 Gb) yoperekedwa ku ma chromosome asanu ndi awiri.Zinthu zobwerezabwereza zimapanga 90.31% ya ma genome ophatikizidwa.Kuwunika kwina kwa msonkhano wa Weining kunawunikiranso kubwereza kwa majini amitundu yonse komanso momwe amakhudzira majini a starch biosynthesis, kapangidwe kake ka prolamin loci, mafotokozedwe a jini omwe amayambira pamutu woyamba komanso zigawo za chromosomal zomwe zimagwirizana ndi kulera komanso loci mu rye.Kutsatira kwa ma genome uku kumalonjeza kupititsa patsogolo maphunziro a genomic ndi kuswana mu rye ndi mbewu zofananira nazo.
2.Rose popanda prickle: chidziwitso cha genomic cholumikizidwa ndi kusintha kwa chinyezi
Malo Ogwirizana: Kunming Institute of botany, Chinese Academy of Sciences
Journal: National Science Review
Zotsatira Zake: 17.273
Mu pulojekitiyi, zitsanzo za 'Basye's Thornless' (BT, kalimidwe kopanda prickle wa Rosa wichuraiana), 'Old Blush' (OB, woyambitsa genotype in rose domestication), ma hybrids awo a F1 ndi BCF1 anasonkhanitsidwa.Ndipo msonkhano wapamwamba kwambiri wa genome udapangidwa kuti uzindikire ma genetic okhudzana ndi chitukuko cha tsinde.Kukula kwa genome ndi pafupifupi 530.6 Mb.Kuti mutsimikizire mtundu wa genome yomwe yasonkhanitsidwa, kusanthula ngati kufananiza kwa mapu a chibadwa, BUSCO, NGS imawerenganso kuyambiranso, kuyerekeza ndi OB haplotype, kutsata kuwongolera kwa zolakwika za m'munsi ndi kuwunika kwa mtengo wamtundu wa LTR Assembly Index (LAI=20.03) kunachitika.Kafukufukuyu akuwonetsa zovuta za cholowa komanso njira zoyendetsera ma prickles tsinde ndipo adatipatsa maziko ndi zida zatsopano zophunzirira rose biology ndi mgodi wa ma molekyulu okhudzana ndi mikhalidwe yofunika.
3.Whole-Genome Sequence of Synthesized Allopolyploids in Cucumis Iwulula Zomwe Zachitika pa Genome Evolution of Allopolyploidization
Malo Othandizira: Nanjing Agricultural University
Journal: Advanced Science
Zomwe Zimakhudza: 16.801
Kafukufukuyu adanenanso zamtundu wapamwamba kwambiri wa allotetraploid wopangidwa pogwiritsa ntchito interspecific hybridization pakati pa nkhaka (C. sativus, 2n = 14) ndi mitundu yake yakutchire (C. hystrix, 2n = 24) ndi kubwereza kwa chromosome, komwe kuli koyamba. kutsata kwathunthu synthetic allopolyploid.Kuphatikizika kwa genome kunagwiritsa ntchito mayendedwe a "PacBio+BioNano+Hi-C+Illumina", zomwe zidapangitsa kukula kwa genome kwa 530.8Mb ndi contig N50 = 6.5Mb.Zowerengera zidaperekedwa ku ma pseudochromosomes 19 ndi ma subgenome.Zotsatira zikuwonetsa kuti kusakanizidwa, m'malo mwa kubwereza kwa ma genome, kumayambitsa kusintha kwakukulu kwa ma genomic mumitundu yonse ya nyukiliya ndi cp.Inanena kuti heterozygosity yokhazikika imapereka C. × hytivus ndi kuwonjezereka kwa kupsinjika maganizo.Zotsatirazi zimapereka chidziwitso chatsopano cha kusinthika kwa mbewu za polyploidy ndikupereka njira yomwe ikuyembekezeka kuswana mbewu zamtsogolo.
4.Comparative Genome Imawunikira Kukula kwa Transposon Mediated Genome ndi Evolutionary Architecture ya 3D Genomic Folding mu Cotton
Malo Othandizira: Huazhong Agricultural University
Journal: Molecular Biology ndi Evolution
Zotsatira Zake: 16.242
Pulojekitiyi idagwiritsa ntchito Nanopore Sequencing kusonkhanitsa ma genome amitundu itatu ya thonje yomwe ndi: Gossypium rotundifolium (K2, genome size = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, genome size = 1.62Gb, 1b10 = M50), ndi 1. G. raimondii (D5, kukula kwa genome = 0.75Gb, contigN50 = 17.04 Gb).Kupitilira 99% mwa ma genome onse atatu adasonkhanitsidwa kudzera mu Hi-C.Zotsatira za kusanthula kwa BUSCO ndi 92.5%, 93.9% ndi 95.4% motsatira.Ziwerengero zonsezi zikuwonetsa kuti ma genomes atatu amsonkhanowo ndiwongoyerekeza.Kuyerekeza kwa ma genome kumawonetsa tsatanetsatane wa kukulitsa kwamtundu wa TE komwe kumathandizira kusiyana kwakukulu kwa kukula kwa ma genome.Kafukufukuyu akuwunikira gawo la kukula kwa ma genome a transposon-mediated pakusinthika kwa mawonekedwe apamwamba a chromatin muzomera.
5.Kusonkhanitsira mulingo wa chromosome ndi kusanthula mbewu za biomass Miscanthus lutarioriparius genome
Malo Ogwirizana: CAS Center for Excellence mu molecular Plant Sciences
Journal: Nature Communications
Zotsatira: 14.912
Pulojekitiyi inanena za msonkhano wa chromosome wa Miscanthus lutarioriparius genome pophatikiza Oxford Nanopore sequencing ndi Hi-C teknoloji.Msonkhano wa 2.07Gb umakhudza 96.64% ya genome, ndi contig N50 ya 1.71 Mb.Pafupifupi 94.30% ya ma pseudochromosomes onse adakhazikika mu 19 pseudochromosomes.Kupyolera mu kuyerekeza ndi ndondomeko ya BAC, kuyesa kwa LAI, kuwunika kwa BUSCO, kugwirizanitsanso ndi deta ya NGS, kukonzanso deta ya transcriptome, ma genome adawunikidwa ngati apamwamba komanso opitilira.Chiyambi cha Allotetraploid cha M. lutarioriparius chimatsimikiziridwa pogwiritsa ntchito centromeric satellite kubwereza.Ma genes obwerezabwereza a M. lutarioriparius amalemeretsedwa osati molingana ndi kuyankha kupsinjika, koma cell wall biosynthesis.Ma gene obwereza mwina amatenga gawo mu C4 photosynthesis ndipo amathandizira ku Miscanthus C4 photosynthesis pa kutentha kochepa.Kafukufukuyu adapereka chidziwitso chofunikira pophunzira za zomera zosatha.
6.Kusonkhana kwa chromosome Camptotheca acuminata genome kumapereka chidziwitso pa chiyambi cha chisinthiko cha camptothecin biosynthesis.
Malo Othandizira: Sichuan University
Journal: Nature Communications
Zotsatira: 14.912
Ntchitoyi inanena za msonkhano wapamwamba kwambiri wa chromosome wa C. acuminata genome, ndi kukula kwa genome 414.95Mb ndi contingN50 1.47Mb.Tidapeza kuti C. acuminata amakumana ndi kubwereza kwa ma genome odziyimira pawokha ndipo majini ambiri amachokerako amagwirizana ndi camptothecin biosynthesis.Kusiyanitsa kogwira ntchito kwa jini la LAMT ndi kusintha kwabwino kwa mitundu iwiri ya SLAS, choncho, zonsezi zinathandizira kwambiri ku camptothecin biosynthesis ku C. acuminata.Zotsatirazo zinagogomezera kufunika kwa msonkhano wapamwamba wa genome pozindikira kusintha kwa majini mu chiyambi cha chisinthiko cha metabolite yachiwiri.
7. Allele-defined genome imasonyeza kusiyana kwa biallelic panthawi yachitukuko cha chinangwa
Malo Ogwirizana: Chinese Academy of Tropical Agricultural Sciences
Journal: Chomera cha Molecular
Zotsatira Zake: 13.162
Pulojekitiyi yasonkhanitsa mtundu wa chinangwa wa chinangwa wokhala ndi contigN50 1.1Mb pogwiritsa ntchito nsanja ya Pacific Biosciences (PacBio).Pambuyo powunikiridwa ndi BUSCO, LAI index, ndi mapu amtundu wapamwamba kwambiri, ma genome ophatikizidwa amatsimikiziridwa ngati chitetezo.Madera osafunikira adadziwika ndipo ma contigs adazikika pa ma pseudochromosomes 18 pogwiritsa ntchito maulalo a Hi-C.Mtundu wapamwamba komanso wodziwika bwino wa chinangwa ndi wofunikira pakuzindikiritsa ma bi-alleles pa ma homologous chromosomes, zomwe zimalola kufufuza kusiyanitsa ndi kulamulira kwa ma bi-alleles ndi mphamvu zawo zoyendetsera chisinthiko.Zinathandizira kukulitsa njira zoweta mu chinangwa ndi mbewu zina za heterozygous.
8. Genomic kuzindikira za kukula msanga kwa paulownias ndi mapangidwe a Paulownia mfiti tsache.
Malo Othandizira: Henan Agricultural University
Journal: Chomera cha Molecular
Zotsatira Zake: 13.162
Pulojekitiyi inasonkhanitsa nyukiliya yapamwamba kwambiri ya Paulownia fortunei, kukula kwa 511.6 Mb, ndi 93.2% ya zotsatizanazo zikugwirizana ndi ma pseudochromosomes 20.Kuchita bwino kwa photosynthetic kumatheka pophatikiza C3 photosynthesis ndi crassulacean acid metabolism pathway, zomwe mwina zidathandizira kuti mitengo ya paulownia ikule mwachangu.Zowonjezera ma genome sequencing a PaWB phytoplasma, kuphatikizapo kuwunika kwa magwiridwe antchito, adawonetsa kuti zotsatira za PaWB-SAP54 zimalumikizana mwachindunji ndi Paulownia PfSPLa, zomwe zimapangitsa kuwonongeka kwa PfSPLa ndi njira yolumikizira ubiquitin ndikupangitsa kuti pakhale tsache la mfiti.Detayo inapereka chidziwitso chofunikira pa biology ya paulownias ndi njira yoyendetsera mapangidwe a PaWB.
Zinyama zamtundu - Kuzindikira mozama za chisinthiko cha zamoyo
1. Genome ya Nautilus pompilius imawunikira kusinthika kwamaso ndi biomineralization.
Malo Ogwirizana: South China Sea Institute of Oceanology, CAS
Journal: Natural Ecology & Evolution
Zotsatira Zake: 15.462
Ntchitoyi idapereka ma genome athunthu a Nautilus pompilius.Ili ndi genome yocheperako pakati pa ma cephalopods otsatizana, omwe ndi 730.58Mb okhala ndi contigN50 = 1.1Mb.Zotsatira zoyesa za BUSCO ndi 91.31%.Kuphatikizidwa ndi transcriptome, proteome, gene family and phylogenetic analysis, genome iyi idapereka chidziwitso chofunikira pazatsopano za cephalopod, monga diso la pinhole ndi biomineralization.Kafukufukuyu adawonetsa kuti kuwonongeka pakukwanira kwa gulu la jini la Hox kungakhale kokhudzana ndi kutha kwa chipolopolo cha Mollusks.Chofunika kwambiri, kusinthika kwamitundu yambiri kuphatikiza kutayika kwa majini, kutsika kodziyimira pawokha komanso kukulitsa kwa mabanja ena amtundu wamtundu wina ndi maulamuliro omwe amalumikizana nawo mwina adapanga kusintha kwa diso la nautilus pinhole.Nautilus genome idapanga chida chofunikira pakukonzanso zochitika zachisinthiko ndi zatsopano zamitundumitundu zomwe zimaumba ma cephalopods omwe alipo.
2.Kusanthula kwa ma genome a Seadragon kumapereka chidziwitso cha phenotype yake komanso momwe amakhudzira kugonana
Malo Ogwirizana: South China Sea Institute of Oceanology, CAS
Journal: Sayansi Yopita patsogolo
Zotsatira Zake: 14.132
Pulojekitiyi ya de novo–anatsatizana majeremusi aamuna ndi aakazi a seadragon wamba (Phyllopteryx taeniolatus) ndi mitundu yofananira nayo, alligator pipefish (Syngnathoides biaculeatus).Kukula kwa genome kwa Phyllopteryx taeniolatus ndi ~659 Mb(♂）ndi ~663 Mb(♀), yokhala ndi contigN50 ya 10.0Mb ndi 12.1mb.kukula kwa genome kwa Phyllopteryx taeniolatus ndi 637 Mb (♂) ndi ~ 648 Mb (♀), ndi contigN50 ya 18.0Mb ndi 21.0Mb.Kupyolera mu kufufuza kwa phylogenetic, seadragon wamba ndi alligator pipefish ndi taxon ya Syngnathinae, ndipo inasiyana pafupifupi 27.3 Ma zapitazo.Mbiri yolembedwa kuchokera kuzinthu zachilendo, zomangika ngati masamba, zikuwonetsa kuti mitundu yambiri ya majini yomwe imakhudzidwa ndikukula komaliza adasankhidwa pamodzi komanso kulemeretsa zolemba zomwe zingathe kukonzanso minofu ndi majini oteteza chitetezo chathupi.Gulu lodziwikiratu lokhala ndi jini ya amhr2y yamwamuna yogawana ndi wamba seadragon ndi alligator pipefish idadziwika.Pulojekitiyi idapereka umboni wofunikira pamaphunziro osinthika osinthika.
Nthawi yotumiza: Sep-19-2022