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Nkhani

Nanopore-based Full-length Transcriptome Sequencing

Nanopore sequencing imadzisiyanitsa ndi nsanja zina zotsatizana, chifukwa ma nucleotides amawerengedwa mwachindunji popanda kaphatikizidwe ka DNA ndipo amapanga kuwerengera nthawi yayitali pa makumi a kilobases.Izi zimapatsa mphamvu kuwerenga kwachindunji kwa zolembedwa zazitali zonse ndikuthana ndi zovuta mu maphunziro a transcriptomic.

√ Kusakonda kotsatizana kocheperako

√ Kuwerenga kwautali kwa cDNA ku maphunziro a jini

√ Zambiri zomwe zimafunikira kuti mupeze zolemba zomwezo

√ Kuzindikiritsa ma isoform angapo pa jini

√ Kuchulukira kwamafotokozedwe pamlingo wa isoform

nanopore-1

Pipeline Yodziwika ya Bioinformatics

Kutalika kwathunthu-trans-bix-1024x254

Milandu Yaposachedwa Yopambana Ndi Biomarker Technologies

1. Kuwunika kofananira kwa zolembedwa zazitali zazitali zimawulula mphamvu zakukula kwa Gnetum luofuense.

Journal: Frontiers in Genetics

Lofalitsidwa: Marichi 2021

Mawu ofunika: MinION |Alternative splicing |APA |lncRNA |Zolemba zolembedwa mosiyanasiyana

Werengani Mawu Athunthu

2. Kusuntha kwapang'onopang'ono kwa ma RNA ang'onoang'ono kuchokera ku mphukira kupita ku mizu mu interspecific heterografts

Journal: Zomera Zachilengedwe

Lofalitsidwa: Jan. 2021

Mawu ofunika: Nanopore |Ilumina |kuzindikira kwakutali kwa mRNA yam'manja

3. Kusanthula kwautali wamtundu wa mizu ya katsitsumzukwa kumawonetsa momwe mamolekyu amayendera kulolerana kwa mchere chifukwa cha bowa wa arbuscular mycorrhizal.

Journal: Zomera Zachilengedwe ndi Zoyeserera

Lofalitsidwa: Jan. 2021

Mawu ofunika: MinION |Zolemba zosiyanasiyana |Zinthu zolembera |Alternative splicing

4. Kusintha kwa Makhalidwe Athupi ndi Kulemba Kwautali Wonse wa Rose (Rosa chinensis) Mizu ndi Masamba Pothana ndi Kupsinjika kwa Chilala

Journal: Zomera ndi Ma cell Physiology

Lofalitsidwa: Oct. 2020

Mawu ofunika: PromethION |Zolemba zosiyanasiyana |Kuneneratu kogwira ntchito |Ma isoform osiyanasiyana |TF ndi lncRNA

5. Kusanthula ndi kuyerekezera kwathunthu kwa PacBio ndi kutsata kwa RNA yochokera ku nanopore ya Arabidopsis transcriptome

Journal: Zomera Njira

Lofalitsidwa: Apr. 2020

Mawu ofunika: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Kulumikizana kwa genome |Chizindikiro cha zolemba |Alternative splicing |SSR |LNCRNA |Isoform quantification

Nkhani ndi Mfundo Zazikulu ikufuna kugawana milandu yaposachedwa kwambiri ndi Biomarker Technologies, kujambula zomwe zakwaniritsa zasayansi komanso njira zodziwika bwino zomwe zimagwiritsidwa ntchito pophunzira.


Nthawi yotumiza: Jan-08-2022

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