BMKCloud Log in
条形 banner-03

Nkhani

T2T GENOME ASSEMBLY, GAP FREE GENOME

1stMitundu iwiri ya Rice1

Mutu: Msonkhano ndi Kutsimikizika kwa Ma Genomes Awiri Opanda Gap a Xian/indica Rice Akuwulula Kuzindikira mu Zomangamanga za Plant Centromere

Doi:https://doi.org/10.1101/2020.12.24.424073

Yolembedwa Nthawi: Januware 01, 2021.

Institute: Huazhong Agricultural University, China

Zipangizo

O. sativa xian/indicamitundu ya mpunga 'Zhenshan 97 (ZS97)' ndi 'Minghui 63 (MH63)

Njira yotsatirira

NGS imawerenga + HiFi imawerenga + CLR imawerenga + BioNano + Hi-C

Zambiri:

ZS97: 8.34 Gb (~ 23x) HiFi imawerenga + 48.39 Gb (~131x ) CLR imawerenga + 25 Gb(~69x) NGS + 2 Maselo a BioNano Irys

MH63: 37.88 Gb (~103x) HiFi imawerenga + 48.97 Gb (~132x) CLR imawerenga + 28 Gb(~76x) NGS + 2 ma cell a BioNano Irys

Chithunzi-1

Chithunzi 1 Ma genome awiri opanda malire a mpunga (MH63 ndi ZS97)

2ndBanana Genome2

Kamutu: Telomere-to-telomere gapless chromosome ya nthochi pogwiritsa ntchito nanopore sequencing

Doi:https://doi.org/10.1101/2021.04.16.440017

Yolembedwa Nthawi: Epulo 17, 2021.

Institute: Université Paris-Saclay, France

Zipangizo

Haploid iwiriMusa acuminatasppmatenda a malaccensis(DH-Pahang)

Njira zotsatirira ndi deta:

HiSeq2500 PE250 mode + MinION/ PromethION (93Gb, ~200X )+ Mapu opangira (DLE-1+BspQ1)

Table 1 Kuyerekeza kwa Musa acuminata (DH-Pahang) genome assemblies

Table1-Kufananiza-kwa-GRCh38-ndi-T2T-CHM13-misonkhano-yaumunthu-genome-assemblies
Chithunzi-Musa-genomes-architecture-comparison

Chithunzi 2 Musa genomes kufananitsa zomangamanga

3rdPhaeodactylum tricornutum genome3

Mutu: Msonkhano wa genome wa Telomere-to-telomere waP
heodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Yolembedwa Nthawi: Meyi 04, 2021

Institute: Western University, Canada

Zipangizo

Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)

Njira zotsatirira ndi deta:

1 Oxford Nanopore minion flow cell + 2 × 75 yophatikizika-mapeto apakati pazotulutsa NextSeq 550 run

Chithunzi-Workflow-kwa-telomere-to-telomere-genome-assembly-1-1024x740

Chithunzi 3 Kayendetsedwe ka ntchito ka telomere-to-telomere genome assembly

4thMunthu CHM13 genome4

Kamutu: Mndandanda wathunthu wa jini la munthu

Doi:https://doi.org/10.1101/2021.05.26.445798

Yolembedwa Nthawi: Meyi 27, 2021

Institute: National Institutes of Health (NIH), USA

Zida: cell line CHM13

Njira zotsatirira ndi deta:

30 × PacBio circular consensus sequencing (HiFi), 120 × Oxford Nanopore Ultra-long read sequencing , 100 × Illumina PCR-Free sequencing (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano ndi Strand-seq

Gulu 2 Kuyerekeza kwa GRCh38 ndi T2T-CHM13 magulu amtundu wa anthu

Table-Kuyerekeza-kwa-Musa-acuminata-DH-Pahang-genome-assemblies

Buku

1.Sergey Nurk et al.Mndandanda wathunthu wa jini la munthu.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2.Caroline Belser et al.Telomere-to-telomere gapless chromosomes ya nthochi pogwiritsa ntchito nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3.Daniel J. Giguere et al.Msonkhano wa telomere-to-telomere genome wa Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4.Jia-Ming Song et al.Msonkhano ndi Kutsimikizika kwa Ma Genome Awiri Opanda Gap a Reference Genomes a Xian/indica Rice Akuwulula Kuzindikira mu Zomangamanga za Plant Centromere.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Nthawi yotumiza: Jan-06-2022

Titumizireni uthenga wanu: