Ma genotyping apamwamba kwambiri, makamaka pa anthu ambiri, ndi gawo lofunikira kwambiri mu maphunziro a ma genetic, omwe amapereka maziko a chibadwa cha kutulukira kwa majini, kusanthula kwachisinthiko, ndi zina zotero. ) imayambitsidwa pofuna kuchepetsa mtengo wotsatizanatsatizana pachitsanzo chilichonse, komanso kukhalabe ndi luso lokwanira pakutulukira kwa chibadwa.Izi nthawi zambiri zimatheka pochotsa chidutswa choletsa mkati mwa kukula kwake, chomwe chimatchedwa laibulale yoyimira (RRL).Specific-locus amplified fragment sequencing(SLAF-Seq) ndi njira yodzipangira yokha yopezera de novo SNP ndi SNP genotyping ya anthu ambiri.
ukadaulo wa ntchito
SLAF vs njira za RRL zomwe zilipo
Ubwino wa SLAF
Kuzindikira kwamphamvu kwa chibadwa- Kuphatikizidwa ndi ukadaulo wapamwamba kwambiri wotsatizana, SLAF-Seq imatha kupeza ma tag mazana masauzande opezeka mkati mwa genome yonse kuti akwaniritse zopempha zamafukufuku osiyanasiyana, kaya ndi kapena ndi genome yathu.
Mapangidwe oyesera osinthidwa mwamakonda & osinthika- Pazolinga zosiyanasiyana zofufuzira kapena mitundu, njira zosiyanasiyana zogayira ma enzymatic zilipo kuphatikiza ma enzyme amodzi, ma enzyme awiri ndi ma enzyme ambiri.Njira yachigayidwe idzawunikiridwatu mu silico kuti zitsimikizire kapangidwe kake ka enzyme.
High bwino mu enzymatic chimbudzi- Kugaya kopangidwa kale kwa enzymatic kumapereka ma SLAF ogawika bwino pa chromosome.Kutolera bwino kwa zidutswa kumatha kukwaniritsa 95%.
Pewani kubwerezabwereza- Peresenti ya kubwereza mobwerezabwereza mu data ya SLAF-Seq imachepetsedwa mpaka 5%, makamaka mwa mitundu yokhala ndi zinthu zobwerezabwereza, monga tirigu, chimanga, ndi zina zotero.
Ntchito yodzipangira yokha bioinformatics- BMK idapanga mayendedwe ophatikizika a bioinformatics omwe amagwiritsidwa ntchito paukadaulo wa SLAF-Seq kuti atsimikizire kudalirika komanso kulondola kwa zotsatira zomaliza.
Kugwiritsa ntchito SLAF
Mapu olumikizana ndi ma Genetic
Kupanga mapu amtundu wapamwamba kwambiri ndikuzindikiritsa malo omwe amawongolera mawonekedwe amaluwa mu Chrysanthemum(Chrysanthemum x morifolium Ramat.)
Journal: Horticulture Research Yosindikizidwa: 2020.7
GWAS
Kuzindikiritsa jini yokhudzana ndi isofavone mumbewu za soya pogwiritsa ntchito ma genome-wide mayanjano ndi mapu olumikizana.
Journal: The Plant Journal Yosindikizidwa: 2020.08
Evolutionary Genetics
Population genomic analysis and de novo assembly ikuwulula magwero a mpunga wa udzu ngati masewera achisinthiko
Journal: Chomera cha Molecular Chosindikizidwa: 2019.5
Bulked Segregant Analysis (BSA)
GmST1, yomwe imakhala ndi sulfotransferase, imathandizira kukana ma virus a soya mosaic G2 ndi G3.
Journal: Chomera, Ma cell & Chilengedwe Chosindikizidwa: 2021.04
Buku
Sun X, Liu D, Zhang X, et al.SLAF-Seq: njira yabwino kwambiri yodziwira zazikulu za de novo SNP ndi genotyping pogwiritsa ntchito kutsata kwapamwamba[J].Plos one, 2013, 8(3):e58700
Song X, Xu Y, Gao K, et al.Kupanga mapu amtundu wapamwamba kwambiri ndikuzindikiritsa malo omwe amawongolera mtundu wamaluwa mu Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020; 7:108.
Wu D, Li D, Zhao X, et al.Kuzindikirika kwa jini yolumikizidwa ndi isoflavone zomwe zili mumbewu ya soya pogwiritsa ntchito ma genome-wide mayanjano ndi mapu olumikizana.Chomera J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.Population Genomic Analysis ndi De Novo Assembly Ziwulula Chiyambi cha Weedy Rice ngati Masewera a Chisinthiko.Zomera za Mol.2019; 12 (5): 632-647.Zomera za Mol.2018;11(11):1360-1376.
Zhao X, Jing Y, Luo Z, et al.GmST1, yomwe imakhala ndi sulfotransferase, imathandizira kukana ma virus a soya mosaic G2 ndi G3.Plant Cell Environ.2021;10.1111/pce.14066
Nthawi yotumiza: Jan-04-2022