MICROBIAL ECOLOGY
Kuchulukitsa kwaulimi kumachepetsa zovuta zama network a microbial komanso kuchuluka kwa msonkho wamtengo wapatali mumizu.
Kutalika Kwa Amplicon Sequencing (ITS) |PacBio HIFI
Mfundo zazikuluzikulu
AKukula kwaulimi kwakhala kovuta kwambiri chifukwa cha zovuta zachilengedwe zomwe zikuphatikizapo kusagwiritsa ntchito bwino kwa zakudya m'thupi, madzi otchedwa goundwater eutrophiation, kuwonongeka kwa nthaka yabwino, ndi zina zotero. Njira zina zaulimi kuphatikizapo kusalima ndi kulima kwachilengedwe zakhala zikugwiritsidwa ntchito kwambiri pofuna kuchepetsa kuvulaza.Ma Microbial Community amatenga gawo lofunikira kwambiri pakupanga ndi kukhazikika kwa agroecosystem.Komabe, sizikudziwika bwino kuti njira zosiyanasiyana zaulimi zimakhudza bwanji mizu ya microbiota.
Mapangidwe Oyesera
Zoyesera
SMafuta ndi mizu (DNA) anali ochokera m'minda ya tirigu kuchokera m'minda 60 yaulimi (20 iliyonse)
Gkugwetsa: 1. Convention (ndi tillage);2. Msonkhano (osalima);3. Mafamu achilengedwe
Sequencing strategy: Full-length amplicon sequencing (ITS)
Pzida: ITS1F-ITS4 (kulunjika dera lonse la ITS ~630 bp)
Sequencing nsanja: PacBio RS II
Bioinformatics Analysis
Zotsatira
On avareji ya ma OTU 357 adadziwika pamalo aliwonse komanso ma OTU okwana 837 pamasamba 60 onse.Kusiyanasiyana kwa ma alpha a mizu ya mafangasi sikunawonetse kusiyana kwakukulu pakati pa machitidwe atatu aulimi.Komabe, magulu atatu osiyana adapangidwa pakuwunika kwamitundu yosiyanasiyana ya beta, zomwe zikuwonetsa zotsatira zamphamvu zaulimi pamizu ya mizu ya mafangasi.
Chithunzi 1. Kusiyanasiyana kwa ma Alpha (chilozera cha Shannon ndi kapangidwe ka anthu) ndi kusanthula kwamitundu yosiyanasiyana ya beta (kusanthula kovomerezeka kwa maupangiri akulu) pamidzi ya mafangasi.
TMisonkho yamwala wofunikira idafotokozedwa kutengera kuchuluka kwa madera a mafangasi pamachitidwe atatu aulimi: malo 10 apamwamba okhala ndi digiri yapamwamba kwambiri, kuyandikira kwambiri pakati komanso pakati papakati kwambiri adasankhidwa.Zisanu ndi ziwiri za izo zinali za mycorrhizal.
Chithunzi 2. Maukonde onse okhudzana ndi mizu ya mafangasi a machitidwe atatu aulimi
Fmaukonde okhudzana ndi zida zankhondo adawonetsa kulumikizana kwapamwamba kwambiri mumaneti wachilengedwe okhala ndi m'mphepete mowirikiza kawiri komanso ma node olumikizidwa kuposa maukonde osagwiritsa ntchito komanso ma network wamba.Kuphatikiza apo, maukonde olima organic amakhala ndi msonkho wamtengo wapatali kwambiri (diamondi) poyerekeza ndi ena onse, omwe adathandizira zovuta zake komanso kulumikizana kwake.
Chithunzi 3. Njira yolima yokhudzana ndi mizu ya mafangasi
AKugwirizana kwakukulu koyipa pakati pa kukula kwaulimi ndi kulumikizana kwa maukonde a fungal network kunawonedwa.Kusanthula kwa nkhalango kwachisawawa kunavumbulutsa madalaivala akuluakulu a msonkho wamtengo wapatali: phosphorous nthaka, kachulukidwe kachulukidwe, pH ndi mycorrhizal colonization.
Chithunzi 4. Kuchulukira kwaulimi ndi kulumikizana kwa maukonde pamitundu itatu yaulimi (A ndi B);Kusanthula nkhalango mwachisawawa(C) ndi Ubale pakati pa ulimi ndi AMF colonization (D)
Zamakono
Kutsatizana kwa amplicon kutalika
A"Kutsatizana kwa M'badwo Wachitatu" kubwera pa siteji, zolepheretsa m'magawo omwe akuwunikiridwa ndi zovuta mu msonkhano wa de novo zathetsedwa.Pacific Bioscience (PacBio) yawonjezera bwino kuwerengera kwa magawo makumi a kilobases, zomwe zimatilola kuti tipeze kuwerenga kwautali kwa 16s rRNA (1,000 bp-1,500 bp) mu mabakiteriya kapena 18S rRNA (1,500 bp-2,000 bp) ndi ITS zigawo (400 bp-900 bp) mu eukaryotic.Kuwona kofalikira kwa ma genetic kunathandizira kwambiri kutsimikizika kwa mitundu yamitundu ndi machitidwe ogwirira ntchito.Nkhani yayitali yokhudzana ndi kulondola kwa maziko yathetsedwa ndi PacBio CCS kudziwongolera, komwe kumapangitsa kuti HIFI iwerengedwe mopitilira 99% molondola.
Kugwira ntchito muzofotokozera za OTU
TKupeza ubwino wa kuwerengera kwautali ndi kupititsa patsogolo, kulondola kwa mawu ofotokozera kungawonjezeke kwambiri ndikukwaniritsa chigamulo cha "mtundu wamtundu" pakuzindikiritsa tizilombo.
Buku
Banerjee, Samiran, et al."Kukula kwaulimi kumachepetsa zovuta zama network a microbial komanso kuchuluka kwa msonkho wamtengo wapatali mumizu."The ISME Journal (2019).
Tech ndi Zowunikira Cholinga chake ndi kugawana njira zamakono zotsatirira bwino kwambiri m'mabwalo osiyanasiyana ofufuza komanso malingaliro anzeru pamapangidwe oyesera ndi migodi ya data.
Nthawi yotumiza: Jan-08-2022