Kuwona momwe ma jini amafotokozera pa malo oyamba a chamoyo ndikofunikira kuti timvetsetse mitundu ndi ntchito za maselo ake.Komabe, njira zamakono zowunikira zolembera za malo zili ndi malire monga kutsika kochepa kapena kusakwanira kokwanira.BMKMANU S1000 Spatial Chip, yopangidwa ndi BMKMANU, imatha kuzindikira chidziwitso cha jini mu-situ m'magawo athunthu amtundu wa subcellular resolution, ndikupangitsa kutanthauzira kosiyanasiyana kwamapangidwe a minofu.
Konzekerani kuti mudzalandire malonda athu atsopano!Kuti tikondwerere kutulutsidwa kwake, tikukupatsani yapaderaGulani Zinayi Pezani Imodzi Yaulerekukwezedwa kwakanthawi kochepa chabe.
Zida Zopangira
Chip cha BMKMANU S1000 ndi zida zofananira zofananira zikuphatikiza:
1) Tissue Optimization Kit, yomwe ili ndi ma reagents kuti azitha kukhathamiritsa minofu (kufufuza kwanthawi yabwino kwa minofu permeabilization).
2) Gene Expression Kit, yomwe ili ndi ma reagents ojambulira mRNA m'magawo a minofu kuti akonzere laibulale ndikutsatizana.
3) Zida Zoyambira: Zimaphatikizapo adapter ya thermostatic ndi transcriptome magnetic separator.
Mfundo Zaumisiri
Chipchi chimakhazikitsidwa ndi ma micropores ndi ma microbeads, ndipo mRNA imatha kugwidwa mu situ kudzera pa Oligo pa ma microbeads.Oligo ili ndi magawo anayi: Read1, Spatial Barcode, UMI, ndi Poly(dT)VN.Minofuyo ikamangirizidwa ku chip, mRNA imatulutsidwa kuchokera ku minofu ndi puloteni ya permeabilization.Popeza mathero ambiri a mRNA 3' ali ndi mchira wa Poly-A, amatengedwa ndi Oligo yokhala ndi Poly(dT)VN.Pambuyo pa RT-PCR ndi cDNA kukulitsa, kukonzekera laibulale ndi kutsatizana, kufufuza malo kumatheka kudzera mu Spatial Barcode.Izi zimalola kusanthula kwa ma jini m'malo komanso kusiyanasiyana kwa minofu.
Ubwino wa Zamalonda
Mapulogalamu
Itha kugwiritsidwa ntchito kumadera ambiri ofufuza zamankhwala ndi zamankhwala, kuphatikiza chotupa, matenda, chitetezo chamthupi, ndi chitukuko cha biology, kuthandiza kukwaniritsa zotsogola zatsopano m'magawo awa ndi kusanthula kwa mawonekedwe apakati pakusintha kwamagulu.
● Chotupa ndi Matenda:
Spatial heterogeneity ndi microenvironment ya zotupa ndi matenda
Kuyamba ndi chitukuko cha zotupa ndi matenda
Chithandizo poyankha zotupa ndi matenda
●Developmental Biology
Spatiotemporal atlas ya ziwalo
Njira zoyendetsera ma gene panthawi ya chitukuko
●Kuchita Zinthu Zolimbitsa Thupi
Kuyankha kwa biotic stress
Kuyankha kwa Abiotic stress
●Madokotala a Immunology
Kuyankha kwa chitetezo chamthupi pakuika chiwalo
Chitetezo cha mthupi cha zotupa ndi matenda
Pathogenesis ya matenda a autoimmune
●Kusanthula kukana mankhwala
Njira zopewera mankhwala
Kafukufuku ndi chitukuko cha mankhwala atsopano
Milandu ndi Demo Data
BMKMANU S1000 yatsimikiziridwa kuti ikugwira ntchito m'ma mazana ambiri amitundu yosiyanasiyana.
