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Metagenomic Sequencing (NGS)
Metagenome imatanthawuza kusonkhanitsa kwa chibadwa chamagulu osakanikirana a zamoyo monga chilengedwe, metagenome yaumunthu, ndi zina zotero. Lili ndi ma genome a tizilombo tomwe timalima komanso tosalimidwa.Metagenomic sequencing ndi chida cha mamolekyu chomwe chimagwiritsidwa ntchito posanthula zinthu zosakanikirana zamtundu wotengedwa kuchokera ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chambiri pamitundu yosiyanasiyana ndi kuchuluka kwake, kuchuluka kwa anthu, ubale wa phylogenetic, majini ogwira ntchito komanso maukonde olumikizana ndi chilengedwe.
nsanja:Illumina NovaSeq6000
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Metagenomic Sequencing-Nanopore
Metagenomics ndi chida cha mamolekyu chomwe chimagwiritsidwa ntchito pofufuza zinthu zosakanikirana zamtundu wamtundu zomwe zimatengedwa kuchokera ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chatsatanetsatane cha mitundu yosiyanasiyana ya zamoyo ndi kuchuluka kwake, chiwerengero cha anthu, ubale wa phylogenetic, majini ogwira ntchito ndi maukonde ogwirizana ndi chilengedwe, ndi zina zotero. ku maphunziro a metagenomic.Kuchita kwake kwapadera pakuwerenga kwanthawi yayitali kunapititsa patsogolo kusanthula kwa metagenomic, makamaka msonkhano wa metagenome.Kutengera ubwino wowerengera kutalika, kafukufuku wa Nanopore-based metagenomic amatha kukwaniritsa msonkhano wopitilirabe poyerekeza ndi kuwombera mfuti.Zasindikizidwa kuti metagenomics yochokera ku Nanopore idapanga bwino ma genome athunthu komanso otsekedwa a bakiteriya kuchokera ku ma microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
nsanja:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit pa 16S ndi 18S rRNA yomwe ili ndi zigawo zonse zotetezedwa kwambiri komanso zosinthika kwambiri ndi chala chabwino kwambiri cha molekyulu yozindikiritsa zamoyo za prokaryotic ndi eukaryotic.Potengera mwayi wotsatizana, ma amplicon awa amatha kuyang'ana pazigawo zosungidwa ndipo zigawo zosinthika zimatha kudziwika bwino kuti tizindikirike ndi tizilombo tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono, taxonomy, phylogeny, ndi zina. ) kutsatizana kwa nsanja ya PacBio kumathandizira kupeza zowerengera zolondola kwambiri, zomwe zimatha kuphimba amplicons aatali (pafupifupi 1.5 KB).Kuwoneka kokulirapo kwa ma genetic kumathandizira kwambiri kusintha kwa mitundu ya mabakiteriya kapena bowa.
nsanja:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing cholinga chake ndi kuwulula phylogeny, taxonomy, ndi kuchuluka kwa mitundu m'magulu ang'onoang'ono pofufuza zinthu za PCR za zolembera zanyumba zomwe zili ndi magawo omwe amakambirana kwambiri komanso osasinthika.Kuyambitsidwa kwa zala zabwino kwambiri zamamolekyu olembedwa ndi Woeses et al, (1977) kumapereka mphamvu zodzipatula za microbiome.Kutsatizana kwa 16S (mabakiteriya), 18S (bowa) ndi Internal transcribed spacer(ITS, bowa) amalola kuzindikirika kwa mitundu yambirimbiri komanso mitundu yosowa komanso yosadziwika.Tekinoloje iyi yakhala chida chogwiritsidwa ntchito kwambiri komanso chachikulu pakuzindikiritsa mitundu yosiyanasiyana ya tizilombo m'malo osiyanasiyana, monga pakamwa pamunthu, matumbo, ndowe, ndi zina zambiri.
nsanja:Illumina NovaSeq6000
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Mabakiteriya ndi mafangasi a Genome Yonse Yotsatizananso
Kutsatizananso kwa ma genome ndi mabakiteriya ndi mafangasi ndi chida chofunikira kwambiri pomaliza ma genome a mabakiteriya odziwika ndi mafangasi, komanso kuyerekeza ma genome angapo kapena kujambula ma genome a zamoyo zatsopano.Ndikofunikira kwambiri kutsata ma genome onse a bakiteriya ndi bowa kuti apange ma genomes olondola, kuti tizindikire tizilombo tating'onoting'ono ndi maphunziro ena ofananiza ma genome.
Platform: Illumina NovaSeq 6000
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Matenda a fungal genome
Biomarker Technologies imapereka kafukufuku wa ma genome, genome yabwino komanso genome yokwanira ya fangasi kutengera cholinga cha kafukufuku wina.Kutsatizana kwa ma genome, kusonkhanitsa ndi kutanthauzira kogwira ntchito kungapezeke mwa kuphatikiza kutsatizana kwa m'badwo Wotsatira + Kutsatizana kwa m'badwo wachitatu kuti mukwaniritse msonkhano wapamwamba kwambiri wa genome.Ukadaulo wa Hi-C utha kugwiritsidwanso ntchito kuti athandizire kuphatikiza ma genome pamlingo wa chromosome.
nsanja:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq 6000
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Mabakiteriya Onse Genome
Biomarker Technologies imapereka ntchito zotsatizana popanga ma genome athunthu a mabakiteriya okhala ndi zero gap.Kayendedwe kake ka mabakiteriya omanga ma genome amaphatikizapo kutsatizana kwa m'badwo wachitatu, kusonkhanitsa, kafotokozedwe ka magwiridwe antchito ndi kusanthula kwapamwamba kwa bioinformatics kukwaniritsa zolinga za kafukufuku.Kufotokozera momveka bwino kwa mabakiteriya amtundu wa mabakiteriya kumapereka mphamvu zowulula zida zofunika kwambiri zomwe zimachokera ku chilengedwe chawo, zomwe zingaperekenso chidziwitso chofunikira pa kafukufuku wamtundu wa mitundu yapamwamba ya eukaryotic.
nsanja:Nanopore PromethION P48 + Illumina NovaSeq 6000
PacBio Sequel II
