Zogulitsa

Metabolomics

Metabolome ndi chinthu chomwe chimatsikira pansi pa jini ndipo chimakhala ndi gawo lonse la mamolekyu otsika kwambiri (metabolites) mu selo, minofu, kapena chamoyo.Metabolomics cholinga chake ndi kuyeza kuchuluka kwa mamolekyu ang'onoang'ono malinga ndi momwe thupi limakhudzidwira kapena matenda.Njira za metabolomics zimagwera m'magulu awiri osiyana: metabolomics osalunjika, kusanthula kwatsatanetsatane kwa zowunikira zonse zomwe zingayesedwe muzachitsanzo kuphatikiza zosadziwika zamankhwala pogwiritsa ntchito GC-MS/LC-MS, ndi ma metabolomics omwe amayang'aniridwa, kuyeza kwa magulu omwe ali ndi mankhwala komanso ma metabolites opangidwa ndi biochemically.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zitsanzo Zofunika

Metabolomics yopanda cholingaMitundu ya zitsanzo		Chitsanzo chovomerezekakuchuluka	Kubwereza kwa Zamoyo
LC-MS/GC-MSmatenda a metabolism	Minofu	200 mg	Chomera ≥6; Zinyama ≥10; Munthu ≥30;
	Plasma / seramu	200ul
	Zamkatimu / M'mimba	150 mg
	Maselo	1*10⁶
	mkodzo	500ul
	Rumen madzi	1 ml
Matenda a metabolomicsMitundu ya zitsanzo		Ndalama zachitsanzo zoyenera	Kubwereza kwa Zamoyo
Phytohormone/Tryptophane/Mphamvu Metabolism / Oxidized lipids / Carotenoid;carotinoid	Minofu	500 mg	Chomera ≥3; Zinyama ≥6;
	Plasma / seramu	500ul
	Fecal	1000 mg
	Maselo	1*10⁷

Mafotokozedwe a Utumiki

Metabolomics yopanda cholinga:
1. Ukadaulo wokhwima komanso wokhazikika, kusamvana kwakukulu komanso kusankha bwino
2. Nawonso database yonse;
3. Oyenera kusanthula matrices ovuta, kupereka chidziwitso chokwanira pakuwunika kumodzi;

Matenda a Metabolomics:
1. Gwiritsani ntchito kutsimikizira kwamkati kwa isotopu kuti mupititse patsogolo kulondola kwazinthu komanso kuchuluka kwa zinthu;
2.Quality control system kuti zitsimikizire kudalirika kwa data.