Zogulitsa

Fungal Genome

Biomarker Technologies imapereka kafukufuku wa ma genome, genome yabwino komanso genome yokwanira ya fangasi kutengera cholinga cha kafukufuku wina.Kutsatizana kwa ma genome, kusonkhanitsa ndi kutanthauzira kogwira ntchito kungathe kupezedwa mwa kuphatikiza kutsatizana kwa m'badwo Wotsatira + Kutsatizana kwa mbadwo wachitatu kuti mukwaniritse msonkhano wapamwamba kwambiri wa genome.Ukadaulo wa Hi-C utha kugwiritsidwanso ntchito kuti uthandizire kuphatikiza ma genome pamlingo wa chromosome.

nsanja:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Ubwino wa Utumiki

● Njira zingapo zotsatirira zomwe zilipo pazolinga zosiyanasiyana za kafukufuku

● Wodziwa zambiri pakupanga ma genome a mafangasi omwe ali ndi ma pene-complete genome opitilira 10,000.

● Gulu lothandizira ukadaulo waukatswiri lomwe limakwaniritsa zofunikira pakufufuza.

Mafotokozedwe a Utumiki

Utumiki	Njira Yotsatirira	Ubwino wotsimikizika	Nthawi yoyerekeza yozungulira
Mapu abwino a fungal	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 masiku ogwira ntchito
Mapu abwino a fungal	PacBio HiFi 30X	Contig N50≥2 Mb	35 masiku ogwira ntchito
Mapu athunthu a fungal pene	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Chromosome anchoring ratio>90%	45 masiku ogwira ntchito

Bioinformatics kusanthula

● Kuwongolera khalidwe la data yaiwisi

● Msonkhano wa genome

● Kusanthula chigawo cha genome

● Kufotokozera kwa jini

● Kuyerekeza kwa ma genomic

Zitsanzo Zofunika ndi Kutumiza

Zitsanzo Zofunika:

ZaZithunzi za DNA:

Mtundu Wachitsanzo	Ndalama	Kukhazikika	Chiyero
Zithunzi za DNA	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Za zitsanzo za minofu:

Mtundu wachitsanzo

Analimbikitsa chitsanzo chithandizo

Zitsanzo zosungira ndi kutumiza

Unicellular bowa

Yang'anani yisiti pansi pa maikulosikopu ndikuwasonkhanitsa mu gawo lawo

Kusamutsa chikhalidwe (chokhala pafupifupi 3-4.5e9 maselo) mu 1.5 kapena 2 ml eppendorf.(Keep on ice)

Centrifuge chubu kwa mphindi 1 pa 14000 g kuti mutenge mabakiteriya ndikuchotsa mphamvu yamphamvu mosamala.

Tsekani chubu ndikuwumitsa mabakiteriya mu nayitrogeni wamadzimadzi kwa maola 1-3.Sungani chubu mu -80 ℃ furiji.

Ikani zitsanzo mu nayitrogeni wamadzimadzi kwa maola 3-4 ndikusunga mu nayitrogeni wamadzimadzi kapena -80 digiri kuti musungidwe nthawi yayitali.Kutumiza kwachitsanzo kokhala ndi ayezi wowuma ndikofunikira.

Macro bowa

Minofu mu mwachangu kukula gawo tikulimbikitsidwa.

Tsukani minofu ndi madzi opanda endotoxin, ndiye 70% ethonal.

Sungani chitsanzo mu cryo-chubu.