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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq imapereka mbiri ya genome-wide ya DNA zomwe zikufuna kusintha histone, transcript factor, ndi mapuloteni ena okhudzana ndi DNA.Imaphatikiza kusankha kwa chromatin immuno-precipitation (ChIP) kuti ipezenso ma protein-DNA complexes, ndi mphamvu ya m'badwo wotsatira (NGS) pakutsatizana kwapamwamba kwa DNA yopezedwa.Kuphatikiza apo, chifukwa ma protein-DNA complexes amatengedwa kuchokera ku maselo amoyo, malo omangira amatha kufananizidwa mumitundu yosiyanasiyana ya maselo ndi minofu, kapena mosiyanasiyana.Mapulogalamuwa amachokera ku malamulo olembera kupita ku njira zachitukuko kupita ku njira za matenda ndi kupitirira.
Platform: Illumina NovaSeq 6000
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Kutsatizana konse kwa genome bisulfite
DNA methylation pamalo achisanu mu cytosine (5-mC) imakhudza kwambiri mafotokozedwe a jini ndi zochitika zama cell.Ma methylation osadziwika bwino amalumikizidwa ndi mikhalidwe ndi matenda angapo, monga khansa.WGBS yakhala muyezo wagolide wophunzirira genome-wide methylation pa single base resolution.
nsanja: Illumina NovaSeq6000
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Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
ATAC-seq ndi njira yotsatirira kwambiri yowunikira kupezeka kwa chromatin ya genome, yomwe ndiyofunikira pakuwongolera epigenetic yapadziko lonse lapansi pakuwonetsa ma jini.Ma adapter otsatizana amalowetsedwa m'magawo otseguka a chromatin ndi hyperactive Tn5 transposase.Pambuyo pakukulitsa kwa PCR, laibulale yotsatizana imapangidwa.Magawo onse a chromatin otseguka amatha kupezeka pansi pa nthawi yeniyeni ya danga, osati kokha kumalo omangira a chinthu cholembera, kapena chigawo china chosinthidwa cha histone.
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Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)
Kafukufuku wa DNA methylation nthawi zonse wakhala mutu wovuta kwambiri pa kafukufuku wa matenda, ndipo umagwirizana kwambiri ndi mafotokozedwe a majini ndi makhalidwe a pheno-typic.RRBS ndi njira yolondola, yothandiza komanso yachuma pa kafukufuku wa DNA methylation.Kulemeretsa kwa zigawo za zilumba za CpG ndi enzymatic cleavage (Msp I), kuphatikizidwa ndi Bisulfite sequencing, kumapereka chidziwitso chapamwamba cha DNA methylation.
Platform: Illumina NovaSeq 6000
