Zogulitsa

BMKMANU S1000 Spatial Transcriptome

Kulinganiza kwa malo kwa maselo kumagwira ntchito yofunika kwambiri pazochitika zosiyanasiyana zamoyo, monga kulowetsedwa kwa chitetezo cha mthupi, kukula kwa mwana wosabadwayo, ndi zina zotero. Kutsatizana kwa malo, komwe kumasonyeza mbiri ya jini ndikusunga zambiri za malo, kwapereka chidziwitso chambiri mu kamangidwe ka minofu ya transcriptome.Ndi chitukuko chaukadaulo, mawonekedwe owoneka bwino a minofu ndi kusiyana kwenikweni kwamawonekedwe amtundu wa ma cell amayenera kuphunziridwa bwino kwambiri.BMKGENE imapereka ntchito yokwanira, yoyimitsa malo amodzi kuchokera ku zitsanzo kupita ku chidziwitso chachilengedwe.

Matekinoloje a Spatial transcriptomics adapatsa mphamvu malingaliro atsopano m'mabwalo osiyanasiyana ofufuza pothetsa mbiri ya jini yokhala ndi zopezeka m'magawo osiyanasiyana.

Spatial transcriptome chip: BMKMANU S1000

Pulatifomu: Illumina NovaSeq Platform

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

BMKMANU S1000 Spatial Transcriptome Technical Scheme

Ubwino wa BMKMANU S1000

1) Kusintha kwa ma cell ang'onoang'ono: Malo aliwonse ojambulidwa amakhala ndi >2 miliyoni Mabala A Barcode okhala ndi mainchesi a 2.5 µm ndi motalikirana wa 5 µm pakati pa malo omwe ali ndi malo, zomwe zimathandiza kusanthula kwa malo okhala ndi ma sub-cellular resolution (5 µm).

2) Kusanthula kwamasinthidwe angapo: Kusanthula kosinthika kwamitundu ingapo kuyambira 100 μm mpaka 5 μm kuti athetse mitundu yosiyanasiyana ya minofu pakuwongolera koyenera.

3) Kufotokozera kwatsatanetsatane: Zolemba zomwe zatengedwa kuchokera pazithunzi zonse zimatha kufufuzidwa, popanda kuletsa kuchuluka kwa majini omwe mukufuna komanso malo omwe mukufuna.

Mafotokozedwe a Utumiki

Library	Njira yotsatirira	Data Output tikulimbikitsidwa
S1000 cDNA library	BMKMANU S1000-Illumina PE150	60Gb / chitsanzo

Zitsanzo Zofunika

Chitsanzo	Nambala	Kukula	Mtengo wa RNA
OCT yophatikizidwa ndi minofu	2-3 midadada / chitsanzo	Pafupifupi.6.8x6.8x6.8 mm³	RIN≥7

Kuti mumve zambiri zachitsogozo chokonzekera zitsanzo ndi kayendedwe ka ntchito, chonde omasuka kulankhula ndi aBMKGENE katswiri

Kuyenda kwa Ntchito Yantchito

Kuyesera kupanga

Kupereka zitsanzo

Yesetsanitu

Kuyika barcode

Kumanga laibulale

Kutsata

Kusanthula deta

Pambuyo pogulitsa ntchito

Zam'mbuyo: 10x Genomics Visium Spatial Transcriptome

Ena: Kutsata kwamtundu wa Zomera/Zinyama Zonse

Deta yopangidwa ndi BMKMANU S1000 imawunikidwa pogwiritsa ntchito pulogalamu ya "BSTMatrix", yomwe idapangidwa palokha ndi BMKGENE, kuphatikiza:

1) Gene expression matrix generation
2) Kukonza zithunzi za HE
3) N'zogwirizana ndi mtsinje wachitatu chipani mapulogalamu kusanthula
4) Paintaneti "BSTViewer" imathandizira kupeza zotsatira zowonera pazosankha zosiyanasiyana.

1.Spot clustering

s1000 (4)