Zogulitsa

Mabakiteriya Onse Genome

Bacteria Wathunthu wa Genome Chithunzi Chowonetsedwa

Biomarker Technologies imapereka ntchito zotsatizana popanga ma genome athunthu a mabakiteriya okhala ndi zero gap.Kuyenda kwakukulu kwa mabakiteriya omanga ma genome kumaphatikizapo kutsatizana kwa m'badwo wachitatu, kusonkhanitsa, kafotokozedwe kantchito komanso kusanthula kwapamwamba kwa bioinformatics kukwaniritsa zolinga za kafukufuku.Kufotokozera mwatsatanetsatane za mabakiteriya genome kumapatsa mphamvu kuwulula njira zofunika kwambiri zomwe zimathandizira kuti chilengedwe chikhalepo, chomwe chingaperekenso chidziwitso chofunikira pakufufuza kwa ma genomic mu mitundu yapamwamba ya eukaryotic.

nsanja:Nanopore PromethION P48 + Illumina NovaSeq Platform

PacBio Sequel II

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Ubwino wa Utumiki

● Njira zingapo zotsatirira zomwe zilipo pazolinga zosiyanasiyana za kafukufuku

● Bakiteriya wathunthu wa genome wokhala ndi 0 Gap wotsimikizika.

● Wodziwa zambiri pakupanga ma genome a mabakiteriya ndipo ma genome opitilira 10,000 aphatikizidwa.

● Gulu lothandizira ukadaulo waukatswiri lomwe limakwaniritsa zofunikira pakufufuza.

Mafotokozedwe a Utumiki

KutsataNjira

Library

Ubwino wotsimikizika

Nthawi yoyerekeza yozungulira

Nanopore 100X + Illumina 50X

Nanopore 20K

Mtengo wa PE150

0 Gap

Masiku 30

PacBio HiFi 30X

PacBio 10K

Bioinformatics kusanthula

● Kuwongolera khalidwe la data yaiwisi

● Msonkhano wa genome

● Kusanthula chigawo cha genome

● Kufotokozera kwa jini

● Kuyerekeza kwa ma genomic

Zitsanzo Zofunika ndi Kutumiza

Zitsanzo Zofunika:

ZaZithunzi za DNA:

Mtundu Wachitsanzo	Ndalama	Kukhazikika	Chiyero
Zithunzi za DNA	＞ 2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Za zitsanzo za minofu:

Mtundu wachitsanzo

Analimbikitsa chitsanzo chithandizo

Zitsanzo zosungira ndi kutumiza

Mabakiteriya

Yang'anani mabakiteriya pansi pa maikulosikopu ndikusonkhanitsa mabakiteriya mu gawo lawo

Tumizani chikhalidwe cha mabakiteriya (chokhala ndi pafupifupi 3-4.5e9 maselo) mu 1.5 kapena 2 ml eppendorf.(Keep on ice)

Centrifuge chubu kwa mphindi 1 pa 14000 g kuti mutenge mabakiteriya ndikuchotsa mphamvu yamphamvu mosamala.

Tsekani chubu ndikuwumitsa mabakiteriya mu nayitrogeni wamadzimadzi kwa mphindi zosachepera 30.Sungani chubu mu -80 ℃ furiji.

Ikani zitsanzo mu nayitrogeni wamadzimadzi kwa maola 3-4 ndikusunga mu nayitrogeni wamadzimadzi kapena -80 digiri kuti musungidwe nthawi yayitali.Kutumiza kwachitsanzo kokhala ndi ayezi wowuma ndikofunikira.