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Zogulitsa

Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)

Kuyesa kwa Chromatin Yotheka Kwambiri Yokhala Ndi Mayendedwe Apamwamba Odutsa (ATAC-seq) Chithunzi Chowonetsedwa
  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
  • Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)

ATAC-seq ndi njira yotsatirira kwambiri yowunikira kupezeka kwa chromatin ya genome, yomwe ndiyofunikira pakuwongolera ma epigenetic padziko lonse lapansi pakuwonetsa ma jini.Ma adapter otsatizana amalowetsedwa m'magawo otseguka a chromatin ndi hyperactive Tn5 transposase.Pambuyo pakukulitsa kwa PCR, laibulale yotsatizana imapangidwa.Magawo onse a chromatin otseguka amatha kupezeka pansi pa nthawi yeniyeni ya nthawi, osati kungokhala ndi malo omangirira a chinthu cholembera, kapena dera linalake losinthidwa la histone.


Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Ubwino wa Utumiki

● Kusanthula Kwambiri: Njira yowunikira akatswiri kuti akwaniritse zosowa zosiyanasiyana zowunikira;

● Zochitika Zambiri: Ntchito mazanamazana zomwe zatsirizidwa bwino mpaka pano, kuphatikiza zitsanzo za nyama ndi zomera.

Mafotokozedwe a Utumiki

Platform: nsanja ya Illumina NovaSeq

Mtundu wa library: ATAC-seq

Kutulutsa kovomerezeka kwa data: ≥20M zowerengedwa

Zitsanzo Zofunika

Mtundu wachitsanzo: Tishu, ma cell amoyo, kapena ma cell oundana ndi zina zotero

Nambala ya cell: ≥ 5 × 105maselo, nambala yolondola ya selo ndiyofunika kwambiri pakuyesa;

Kulemera kwa minofu: ≥ 200mg minofu yatsopano;

Magazi: ≥ 2 mL

Kuyenda kwa Ntchito Yantchito

Chitsanzo cha QC

Kuyesera kupanga

kutumiza chitsanzo

Kupereka zitsanzo

Kuyesera koyendetsa ndege

Kutulutsa kwa DNA

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito

  • Zam'mbuyo:
  • Ena:

    • 表观-02

    1. Heatmap pa ATAC imawerenga kugawa ku TSS ndi madera oyandikana nawo (± 3 kb)

    3 (1)2.Kugawidwa kwa chigawo cha chromatin chotseguka m'magulu osiyanasiyana a genome

    4 (1)

    3.Kuyitana kwakukulu kosiyana pakati pa magulu

    5 (1)

    pezani mtengo

    Lembani uthenga wanu apa ndikutumiza kwa ife

    Zogulitsamagulu

    Titumizireni uthenga wanu:

    Dinani Enter kuti mufufuze kapena ESC kuti mutseke