Complex and variable alternative isoforms in organisms are important genetic mechanisms for regulating gene expression and protein diversity. Accurate identification of transcript structures is the basis for in-depth study of gene expression regulation patterns. Nanopore sequencing platform has successfully brought transcriptomic study to isoform-level. This analysis platform is designed to analyze RNA-Seq data generated on Nanopore platform on base of reference genome, which achieves qualitative and quantitative analyses in both gene level and transcripts level.