Te Whakaraupapa Ira Katoa Te Tipu/Karehe
1.Ratonga Painga
He wheako nui ki te raupapa ira mo te neke atu i te 1000 momo.
Neke atu i te 800 nga keehi kua whakaputaina me te nui o te paanga whakahiato neke atu i te 4000.
Te tātari bioinformatics matawhānui mo te waea rereke me te tātari mahi.
2. Whakaritenga Ratonga
| Paerewa | Whare Pukapuka | E taunakitia ana te hohonutanga | |
| Maramarama | PE 150 | Mo te SNP, InDel te waea ≥ 10x Mo te SV, CNY te waea ≥ 30x
| |
| Nanopore
| 8 kb | Mo te SV, CNY te waea ≥ 20x | |
| Pacbio | CCS | 15 kb | Mo te SNP, InDel, SV, CNY te waea ≥ 10x |
3. Tauira Whakaritenga
| Paerewa | Conc.(ng/μL)
| Te moni (ng)
| Maamaa
| Ko te kara o te Agarose
| ||
| OD260/280 | OD260/230 | 1. Maamaa te roopu matua me te kore he iti raneika kitea te paheketanga i runga i te gel. 2. Karekau, he iti ranei te RNA, te poke pūmua ranei
| ||||
| Maramarama | ≥1 | ≥30
| - | - | ||
| Nanopore
| ≥30 | Kei runga i te tukunga raraunga 10μg/pūtau | 1.7-2.2 | ≥1.5 | ||
| Pacbio | CCS | ≥50 | 1.7-2.2 | 1.8-2.5 | ||
4. Te Whakamaramatanga o te Mataora
5. Rere Mahi Mahi
Te tuku tauira
tangohanga DNA
Te hanga whare pukapuka
Whakaraupapa
Te tātari raraunga
Te tuku raraunga
1) Te Tauanga o te Mahere Genome
Ripanga 1 Te tatauranga o te hua mapi
Whakaahua 1 Te tohatoha o te rahi whakauru me te panui panui.
2) Rapu rereke
Whakaahua 2 Nga tatauranga me te tuhipoka o SNP/INDEL/SV i waenga i nga Tauira
Whakaahua 3 Te tohatoha ira-whanui o nga waahi
3) Tuhituhi mahi o nga rereketanga
| 2019 | Nga korero mo te taiao | Ko te resequencing-ira-ira katoa e whakaatu ana i te takenga mai o Brassica napus me te waahi ira i whai waahi ki te whakapai ake |
| 2020 | PNAS | Te takenga mai o te kukuwhatanga me te hitori o te kainga o te ika koura (Carassius auratus) |
| 2021 | Te Tuhituhi Biotechnology Plant | Tohutoro tohutoro o nga momo jute e rua kua whakatipuria |
| 2021 | Te Tuhituhi Biotechnology Plant | Nga waitohu ira o te huawhenua me te kakano hinu allopolyploid Brassicajuncea me te waahi ira e whakahaere ana i te whakaemi o nga glucosinolates |
| 2019 | Tipu Molecular | Ko te resequence-genome-katoa o te kohinga o te ao katoa o nga whakaurunga rapeseed e whakaatu ana i te putake ira o te rerekeetanga o te momo rauropi. |
| 2022 | Rangahau Ahuone | Ko te tātari hononga-a-nui-a-ira e whakaatu ana i nga tirohanga irapoi ki te rereketanga o te rahi o te kakano merengi |
| 2021 | Journal of Experimental Botany | Ko te rangahau hononga-a-nui-a-ira e whakaatu ana i nga momo rereke o te GhSAD1 e tuku ana i te maeke makariri i roto i te miro |
| 2021 | Journal of Experimental Botany | Ko te rangahau hononga-a-whanui me te whakatairite tuhinga e whakaatu ana i te pukapuka QTL me nga ira kaitono e whakahaere ana i te rahi o te puapua i roto i te rapeseed. |
