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Epigenetics

  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    Te Whakaraupapa Whakatairanga Chromatin Immunoprecipitation (ChIP-seq)

    Ko te ChIP-Seq e whakarato ana i te tohu ira-a-whanui o nga whaainga DNA mo te whakarereketanga histone, nga waahanga tuhi, me etahi atu pūmua e pa ana ki te DNA.Ka whakakotahihia e ia te kowhiringa o te chromatin immuno-precipitation (ChIP) ki te whakahoki mai i nga kohinga pūmua-DNA motuhake, me te kaha o te raupapa-whakatupuranga-whakatupuranga (NGS) mo te whakahiatotanga teitei o te DNA whakaora.I tua atu, na te mea ka hoki mai nga kohinga pūmua-DNA mai i nga pūtau ora, ka taea te whakataurite nga waahi here ki nga momo pūtau me nga kopa rereke, i raro ranei i nga ahuatanga rereke.Ko nga tono mai i nga ture tuhi ki nga huarahi whanaketanga ki nga tikanga mate me tua atu.

    Paerewa: Illumina NovaSeq 6000

  • Whole genome bisulfite sequencing

    Te raupapatanga bisulfite genome katoa

    Ko te methylation DNA i te tuarima o te tuunga i roto i te cytosine (5-mC) he awe nui ki te whakaatu ira me te mahi pūtau.Ko nga tauira methylation rerekee he maha nga ahuatanga me nga mate, penei i te mate pukupuku.Ko te WGBS te paerewa koura mo te ako i te methylation ira-whanui i te taumira turanga kotahi.

    Paerewa: Illumina NovaSeq6000

  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

    Te Whakatairanga mo te Chromatin Whakawhiti-Uhoe me te Whakaraupapa Whakaraupapa Tino (ATAC-seq)

    Ko te ATAC-seq he tikanga whakahiato teitei mo te wetewete i te urunga o te chromatin genome-wide, he mea nui mo te mana epigenetic o te ao o te whakaaturanga ira.Ka whakaurua nga urutau raupapa ki nga rohe chromatin tuwhera ma te Tn5 transposase hyperactive.Whai muri i te whakakaha PCR, ka hangaia he whare pukapuka raupapa.Ko nga rohe chromatin tuwhera katoa ka taea te tiki i raro i tetahi ahuatanga mokowā-waahi, ehara i te mea iti noa ki nga waahi here o tetahi take tuhi, tetahi rohe whakarereke histone ranei.

  • Reduced Representation Bisulfite Sequencing (RRBS)

    Whakaitihia te Whakaaturanga Bisulfite Sequencing (RRBS)

    Ko te rangahau methylation DNA he kaupapa wera tonu i roto i te rangahau mate, a, he hononga tata ki te whakaaturanga ira me nga ahuatanga tohu-ahua.Ko te RRBS he tikanga tika, pai me te ohanga mo te rangahau methylation DNA.Ko te whakarangatira i nga rohe o nga moutere o te kaiwhakatairanga me te CpG na roto i te wehenga enzymatic (Msp I), ka honoa me te raupapatanga Bisulfite, e whakarato ana i te kitenga methylation DNA taumira teitei.

    Paerewa: Illumina NovaSeq 6000

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