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Huakita me te harore Katoa te Whakaraupapa Anō i te Genome

Huakita me te Fungal Genome Katoa Re-Sequencing Atahanga Whakaatuhia

He taputapu tino nui te whakaraupapa-a-ira-a-ira o te huakita me te harore ki te whakaoti i nga ira o te huakita me te harore e mohiotia ana, me te whakatairite i nga momo ira tangata maha, ki te mapi ranei i nga momo rauropi hou.He mea nui ki te raupapa ira ira katoa o te huakita me te harore kia taea ai te whakaputa ira tohutoro tika, ki te mahi tohu moroiti me etahi atu rangahau iraira whakatairite.

Pūhara: Illumina NovaSeq Platform

Whakapā mai

Nga Taipitopito Ratonga

Whakatakotoranga Ratonga

Paerewa: NovaSeq 6000 turanga, PE150

Momo whare pukapuka: 350bp te whakauru i te puna cDNA (e whakawhirinaki ana ki te tohatoha tihi)

Rautaki Whakaraupapa: Takirua-Whakamutunga 150 bp

Tohua te whakamaarama raupapa: ≥100×

Nga Whakaritenga Tauira

Te nui o te DNA Genomic:≥ 3ug

Te Kukuhanga DNA Genomic: ≥ 20ng/μL

Te Rerenga Mahi Ratonga

tuku tauira

Te tuku tauira

Te Whakaritenga Whare Pukapuka

Te hanga whare pukapuka

Whakaraupapa

Whakaraupapa

Te tātari raraunga

Te tātari raraunga

I muri i te hoko Ratonga

Nga ratonga muri-hoko

Tōmua: PacBio-Katoa-roa 16S/18S/ITS Whakaraupapa Amplicon

Panuku: 16S/18S/ITS Amplicon Sequencing-NGS

whiwhi korero

Tuhia to korero ki konei ka tukuna mai ki a matou

Huangā kāwai

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