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T2T GENOME ASSEMBLY, GAP FREE GENOME

1stGenome vary roa1

Lohateny: Fivoriambe sy Fanamarinana Genome Reference roa tsy misy Gap ho an'ny vary Xian/indica dia manambara ny fomba fijery momba ny Architecture Centromere Plant

Doi:https://doi.org/10.1101/2020.12.24.424073

Navoaka ny fotoana: Janoary 01, 2021.

Institut: Huazhong Agricultural University, Sina

Akora

O. sativa xian/indicakarazana vary 'Zhenshan 97 (ZS97)' sy 'Minghui 63 (MH63)

Strategy sequencing

Mamaky NGS + Mamaky HiFi + Mamaky CLR + BioNano + Hi-C

Data:

ZS97: 8,34 Gb(~23x)HiFi mamaky + 48,39 Gb (~131x) CLR mamaky + 25 Gb(~69x) NGS + 2 BioNano Irys sela

MH63: 37,88 Gb (~103x) HiFi mamaky + 48,97 Gb (~132x) CLR mamaky + 28 Gb(~76x) NGS + 2 BioNano Irys sela

Sary-1

Sary 1 Tanora vary roa tsy misy elanelana (MH63 sy ZS97)

2ndBanana Genome2

Lohateny: Telomere-to-telomere krômôzôma amin'ny akondro tsy misy elanelana amin'ny alàlan'ny filaharan'ny nanopore

Doi:https://doi.org/10.1101/2021.04.16.440017

Fotoana navoaka: Aprily 17, 2021.

Institut: Université Paris-Saclay, France

Akora

Double haploidMusa acuminatasppmalaccensis(DH-Pahang)

Paikady sy angona momba ny filaharana:

Mode HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ Sarintany Optical (DLE-1+BspQ1)

Tabilao 1 Fampitahana ny fivorian'ny genome Musa acuminata (DH-Pahang).

Table1-Fampitahana-n'ny-GRCh38-sy-T2T-CHM13-fikambanana-genome-olombelona
Figure-Musa-genomes-architecture-comparison

Figure 2 Musa génomes architecture fampitahana

3rdPhaeodactylum tricornutum génom3

Lohateny: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Navoaka ny fotoana: May 04, 2021

Institiota: Western University, Canada

Akora

Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)

Paikady sy angona momba ny filaharana:

1 Oxford Nanopore minION sela mikoriana + 2 × 75 mitambatra faran'ny afovoany vokatra NextSeq 550 hazakazaka

Figure-Workflow-for-telomere-to-telomere-genome-assembly-1-1024x740

Figure 3 Workflow for telomere-to-telomere genome assembly

4thGenome CHM13 olombelona4

Lohateny: Ny filaharana feno momba ny génome olombelona

Doi:https://doi.org/10.1101/2021.05.26.445798

Fotoana navoaka: May 27, 2021

Institute: National Institutes of Health (NIH), Etazonia

Fitaovana: cell line CHM13

Paikady sy angona momba ny filaharana:

30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano sarintany optika, ary Strand-seq

Tabilao 2 Fampitahana ny fivorian'ny genome olombelona GRCh38 sy T2T-CHM13

Table-Comparison-of-Musa-acuminata-DH-Pahang-genome-assemblies

Reference

1. Sergey Nurk et al.Ny filaharana feno momba ny génome olombelona.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2. Caroline Belser et al.Telomere-to-telomere gapless chromosomes amin'ny akondro mampiasa nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3. Daniel J. Giguere et al.Telomere-to-telomere genome assembly of Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4.Jia-Ming Song et al.Ny Fivoriambe sy ny Fanamarinana ny Genome Reference roa tsy misy elanelana ho an'ny vary Xian/indica dia manambara ny fomba fijery momba ny Architecture Centromere Plant.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Fotoana fandefasana: Jan-06-2022

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