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Metabolomics

The metabolome is the terminal downstream product of the genome and consists of the total complement of all the low-molecular-weight molecules (metabolites) in a cell, tissue, or organism. Metabolomics aims to measure a wide breadth of small molecules in the context of physiological stimuli or disease states. Metabolomics methodologies fall into two distinct groups: non-targeted metabolomics, an intended comprehensive analysis of all the measurable analytes in a sample including chemical unknowns using GC-MS/LC-MS, and targeted metabolomics, the measurement of defined groups of chemically characterized and biochemically annotated metabolites.


Service Details

Sample Requirements

Non-targeted metabolomics Sample types

Recommended sample amount

Biological Repetition

LC-MS/GC-MS untargeted metabolomics

Tissues

200mg

Plant ≥6;

Animal ≥10;

Human ≥30;

Plasma/serum

200ul

Fecal/Intestinal Contents

150mg

Cells

1*106

urine

500ul

Rumen fluid

1ml

Targeted metabolomics Sample types

Recommended sample amount

Biological Repetition

Phytohormone/Tryptophane/Energy Metabolism/ Oxidized lipids/ Carotenoid; carotinoid

Tissues

500mg

Plant ≥3;

Animal ≥6;

Plasma/serum

500ul

Fecal

1000mg

Cells

1*107

Service Specifications

Non-targeted metabolomics:
1.Mature and stable technology,high resolution and good selectivity
2. Complete database;
3. Suitable for the analysis of complex matrices, providing comprehensive information in one analysis;

Targeted metabolomics:
1.Use isotope internal standard verification to improve the qualitative and quantitative accuracy of substances;
2.Quality control system to ensure the reliability of data.

Service Work Flow

1.Non-targeted metabolomics

11

2.Targeted metabolomics

22

3.Widely targeted metabolomics

33

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