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10x Single Cell
, Microbial Genomes
, 16s Metagenomic Sequencing
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Massive Selection for Shotgun Metagenomics Sequencing -
Small RNA sequencing – Illumina – Biomarker Detail:
Service Advantages
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Contamination |
Amount |
OD260/280≥1.7-2.5; OD260/230≥0.5-2.5; |
For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation |
Limited or no protein or DNA contamination shown on gel. |
Conc. ≥300 ng/μl; Volumn ≥ 10 μl; Total ≥ 3 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.
Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
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Massive Selection for Shotgun Metagenomics Sequencing -
Small RNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Zimbabwe
, Armenia
, Bhutan
, We have advanced production technology, and pursuit innovative in goods. At the same time, the good service has enhanced the good reputation. We believe that as long as you understand our product, you need to be willing to become partners with us. Looking forward to your inquiry.