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Massive Selection for Shotgun Metagenomics Sequencing - Hi-C based Genome Assembly – Biomarker

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Massive Selection for Shotgun Metagenomics Sequencing - Hi-C based Genome Assembly – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality contro
l
ü Hi-C library quality control

ü Hi-C based genome assembly

ü Post-assembly evaluation

2Hi-C-based-genome-assembly

Sample Requirements and Delivery

Sample Requirements:

For animals:
1.Muscle (Frozen): ≥ 2 g;
2.Whole blood (Non-frozen): ≥ 2 ml (for 3 libraries)

For plants:
1.Seedlings (e.g. young seedlings cultivated from seeds, tissue culture seedingling, etc) : collect all leaves and young stem;
2.Mature plant: collect stem tip and the first 1 or 2 new leaves close to stem tip.
* Recommended amount for each sample ≥ 4 g. (Theoretical usage for library construction is 1 g per library).

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)
For most of samples, we recommend not to preserve in ethanol.
Sample labeling: Samples need to be clearly labeled and identical to submitted sample information form.
Shipment: Dry-ice: Samples need to be packed in bags first and buried in dry-ice.

Service Work Flow

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Experiment design

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Sample delivery

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Hi-C experiment

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Library construction

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Sequencing

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Data analysis

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After-sale services

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