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Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker


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Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

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Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:   

For DNA extracts: 

Sample Type

Amount

Concentration

Purity

DNA extracts

> 30 ng

> 1 ng/μl

OD260/280= 1.6-2.5

For environmental samples:

Sample type

Recommended sampling procedure

Soil

Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.

Feces

Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.

Intestinal contents

Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.

Sludge

Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation

Waterbody

For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.

Skin

Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures

Manufactur standard Metabarcoding Service -
 Nanopore-based metagenome sequencing – Biomarker detail pictures


Related Product Guide:

With our rich working experience and thoughtful companies, we have now been recognized as being a trustworthy supplier for a lot of global potential buyers for Manufactur standard Metabarcoding Service - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Zimbabwe , Zurich , Hyderabad , With the advanced workshop, professional design team and strict quality control system, based on mid- to high-end marked as our marketing positioning, our products are fast selling onto European and American markets with our own brands such as below Deniya, Qingsiya and Yisilanya.
  • The company keeps to the operation concept "scientific management, high quality and efficiency primacy, customer supreme", we have always maintained business cooperation. Work with you,we feel easy!
    5 Stars By Barbara from Maldives - 2018.06.21 17:11
    Managers are visionary, they have the idea of "mutual benefits, continuous improvement and innovation", we have a pleasant conversation and Cooperation.
    5 Stars By David Eagleson from Nepal - 2017.09.28 18:29

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