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Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker

Service Details

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We have now sophisticated machines. Our solutions are exported to the USA, the UK and so on, enjoying a great reputation amid consumers for Resequencing Service , Bulk Dna Sequencing , Expression Analysis , Hope we are able to produce a far more superb long term with you by our efforts from the foreseeable future.

Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150	6 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.	For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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The key to our success is "Good Product Excellent, Reasonable Rate and Efficient Service" for Manufactur standard Metabarcoding Service - Eukaryotic mRNA sequencing-Illumina – Biomarker , The product will supply to all over the world, such as: Slovakia , Germany , United States , If any product meed your demand, remember to feel free to contact us. We're sure your any inquiry or requirement will get prompt attention, high-quality merchandise, preferential prices and cheap freight. Sincerely welcome friends all over the world to call or come to visit, to discuss cooperation for a better future!

Circrna Database

Dna Sequencing Analysis

Operational Taxonomic Unit

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Rna Sequencing Service

Small Rna Sequencing

In China, we have many partners, this company is the most satisfying to us, reliable quality and good credit, it is worth appreciation.

By Kim from Suriname - 2017.08.18 18:38

The company has rich resources, advanced machinery, experienced workers and excellent services, hope you keep improving and perfecting your products and service, wish you better!

By Rita from Chile - 2017.10.13 10:47