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Manufactur standard Chloroplast Function - Full-length mRNA sequencing-Nanopore – Biomarker

Service Details

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Manufactur standard Chloroplast Function - Full-length mRNA sequencing-Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Alternative splicing

ü Expression quantification in gene level and isoform level

ü Differential expression analysis

ü Function annotation and enrichment (DEGs and DETs)

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel.	For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥40 ng/μl;Total ≥ 1 μg

Tissue: Weight(dry): ≥1 g

*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷

*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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We insist on the principle of development of 'High quality, Efficiency, Sincerity and Down-to-earth working approach' to provide you with excellent service of processing for Manufactur standard Chloroplast Function - Full-length mRNA sequencing-Nanopore – Biomarker , The product will supply to all over the world, such as: Sheffield , Monaco , Luxembourg , Really should any of these items be of interest to you, please let us know. We will be pleased to give you a quotation upon receipt of one's detailed specifications. We've our personal specialist R&D enginners to meet any of the requriements, We look forward to receiving your enquires soon and hope to have the chance to work together with you inside the future. Welcome to take a look at our organization.

Advanced Bioinformatics