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Parvus RNA sequencing-Illumina

Moleculae parvae RNA (sRNA) moleculae, sub 200 nucleotides typice in longitudine, microRNAs (miRNAs), parvae RNAs (siRNAs) impedimento, et piwi-RNAs (piRNAs).Inter eas, miRNAs, circa 20-24 longi nucleotides, notabiles sunt ob functiones regulatorias suas in variis processibus cellulosis.Cum exemplaria textus-specialis et specifica expressionis scaenicis, miRNAs altam conservationem exhibent per diversas species.

Platform: Illumina NovaSeq


Service Details

Bioinformatics

Demo Results

Featured Publications

Features

Size lectio RNA prior ad bibliothecam praeparationem

Analysis bioinformatica circa miRNA praedictio et scuta sitas

Servitium Commoda

Analysis comprehensiva bioinformatica:ut identificatio mirabilium tam notarum quam noverum, miRNAs scutorum agnitio ac functionis annotationis ac locupletatia cum multiplicibus databases (KEGG, GO) respondeat.

Rigorous Quality Control: medias potestates efficiendi puncta per omnes aetates, a praepatione specimen et bibliothecam ad sequencias et bioinformaticas.Haec vigilantia exquisita efficit ut traditionem constanter summus qualitas consequitur.

Post-Sales Support: Munus nostrum ultra project complementum cum 3-mensibus post venditionis tempus servitutis extenditur.Hoc tempore consilium praebemus subsequens, fermentum subsidia, et sessiones Q&A ad quaslibet quaestiones pertinentes ad proventus.

Expertise: cum semita record of feliciter claudendo plura sRNA projects super 100 species in variis investigationibus ditionibus obtegens, turma experientiae opes ad omne consilium adfert.

Sample Requisita et Delivery

Bibliotheca

Platform

Commendatur data

Data QC

Magnitudine electus

Illumina SE50

10M-20M legit

Q30≥85%

Sample Requisita:

Nucleotides:

Conc.

Moles (μg)

Puritas

Integritas

≥ 80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Nullam dapibus vel dapibus vel DNA contagione gel ostensum est.

RIN≥6.5;

5.0≥28S/18S≥1.0;

limitata vel non collocantur elevatione

● Plantae:

Radix, Caulis vel Petal: 450 mg

Folium vel Semen: 300 mg

Fructus: 1.2 g

Animal:

Cor vel Intestinum: 450 mg

Viscera vel cerebrum: 240 mg

Musculus: 600 mg

Ossa, capillus vel cutis: 1.5g

Arthropoda:

Insecta: 9g

Crustacea: 450 mg

totum sanguinem: 2 tube

Cellulae: 106 cellulae

● Serum et Plasma:6 mL

Sample Delivery commendatur

Continens:
2 ml tubum centrifugium (foil plumbi non commendatur)
Sample labeling: Group+replicata eg A1, A2, A3;B1, B2, B3...

Shipment:
1.Dry-glacies: Exemplaria in saccis referta necesse est et in glacie siccitate defossa.
2.RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperatura exsiccata sunt.

Service Opus O

Sample QC

Experimentum design

Sample partus

Sample traditio

Gubernator experimentum

RNA extractionem

Bibliotheca Praeparatio

Library construction

Sequencing

Sequencing

Analysis

Analysis

Post venditionis Services

Post-venditionis officia


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  • Bioinformatics

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    Mirna sativum: structura et profunditas

     

     

     miRNA praecursor structura et sequentia profunditas

     

    Mirna differentialis expressio - pampineis hiearchicis

    34

     

    Eget annotationem scopi differentialiter miRNAs expressi

    35

    Progressiones investigationis explorare faciliores per BMKGene' sRNA sequencia officia per collectionem publicationum curatarum.

      

    Chen, H. et al.(2023) Infectio viralis inhibet saponin biosynthesi et photosynthesi in Panax notoginseng', Physiologiae Plantae et Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023) 'Herba FYVE domain (continens dapibus FREE1> addit cum componentibus microprocessoris ad reprimendum miRNA biogenesis', EMBO tradit, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. et al.(2023) ' MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Targeting the Multiple Epidermal Augmentum Factor-simile Dominia 8 Gene (megf8) in Honeybee, Apis mellifera', Acta Internationalis Scientiarum Molecularium, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.

    Zhang, M. et al.(2018) 'Analysis Mirnae et Genes Associata cum Meat Quality Revals that Gga-MiR-140-5p Affectus intramusculares Fat Depositio in Pullos', Physiologiae Cellulae et Biochemisticae, 46(6), pp.doi: 10.1159/000489649.

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