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Eukaryotic mRNA Sequencing-Illumina

mRNA sequena efficit ut programmata omnium mRNAs e cellulis sub certis conditionibus transcripta.Magna technologia est ad declarandas gene expressiones figuras, structuras gene structuras et machinationes hypotheticas quarundam processuum biologicorum.Hodie, mRNA sequena late adhibita est in investigationibus fundamentalibus, diagnosticis clinicis, progressu medicamento, etc.

Platform: Illumina NovaSeq Platform


Service Details

Bioinformatics

Demo Results

commoda

● Valde Expertus: Plus 200,000 exempla in BMK sunt discursum diversarum specierum specierum obtegentes, inter cellam culturam, textus, corpus fluidum, etc. et supra 7000 mRNA-Seq incepta clausa operientes varias investigationis aream.

Strictioris qualitas temperantia ratio: Core qualitas puncta per omnes gradus quos possidet praeparatio specimen, bibliotheca praeparatio, sequencia et bioinformatica sub magna vigilantia sunt ut summus qualitas eventus eruat.

● Multiplices databases praesto sunt ad functionem annotationem et locupletationem studiorum ad varias investigationes proposita implendas.

● Post-venditionis officia: Post-sale officia valida per 3 menses ad complementum project, inter incepta subsequens, tribulatio-jaculatio, eventus Q&A, etc.

Sample Requisita et Delivery

Bibliotheca Sequencing strategy Data commendatae Regimen quālitātis
Poly A locupletatus Illumina PE150

6 Gb*

Q30≥85%

Sample Requisita:

Nucleotides:

Conc.

Moles (μg)

Puritas

Integritas

≥ 20

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Nullam dapibus vel dapibus vel DNA contagione gel ostensum est.

Pro plantis: RIN≥6.5;

Pro animalibus: RIN≥7.0;

5.0≥28S/18S≥1.0;

limitata vel non collocantur elevatione

TEXTUS: pondus (siccum);≥1 g
*Pro texti minore quam 5 mg, commendamus ut mico congelato (in liquido nitrogen) texti specimen mittat.

Cellula suspensio:Cellula comitem = 3×106- 1×107
* Commendamus navem gelidam cellam lysatam.In casu cellula illa minor quam 5-105 numerat.

Sanguinis exempla:Volume≥1 ml

Microorganismus:Missa ≥ 1 g

Sample Delivery commendatur

Continens: 2 ml tubum centrifugium (foil plumbi non commendatur)

Sample labeling: Group+replicata eg A1, A2, A3;B1, B2, B3...

Shipment:

  1. Siccum-glacies: Exemplaria in saccis referta necesse est et in glacie siccitate sepeliri.
  2. RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperie conscendunt.

Service Opus O

Sample QC

Experimentum design

Sample partus

Sample traditio

Gubernator experimentum

RNA extractionem

Bibliotheca Praeparatio

Library construction

Sequencing

Sequencing

Analysis

Analysis

Post venditionis Services

Post-venditionis officia


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  • Bioinformatics

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    Eukaryotic mRNA sequentia analysis workflow

    Bioinformatics

    ØRudis notitia qualis imperium

    ØGenome alignment Reference

    ØAnalysis transcript structuram

    ØExpressio quantitatis

    ØDistinctio analysis expressio

    ØMunus annotationis et locupletationis

    1.variant data Saturationis curvae

    3(1)

    2.Analysis differentialis expressio - Volcano insidiarum

    4(1)

    3.KEGG ANNOTATIONES IN DEGs

    5(1)

    4.GO genus in DEGs

    6(1)

    a Quote

    Epistulam tuam hic scribe et mitte nobis

    Epistulam tuam nobis mitte;