circRNA sequencing-Illumina
Features
● rRNA deperditionem secuta est praeparatio bibliothecae directionalis, ut notitias sequendas in acia specialia.
● Bioinformatic workflow dat circRNA praedictionem et quantitatem expressionis
Servitium Commoda
●Amplior RNA libraries:rRNA deperditionem pro linearibus RNA deperditionem in praeparatione nostra prae-bibliaria utimur, ut sequentia notitia non solum circRNA, sed etiam mRNA et lncRNA comprehendat, ut iuncturam analysin in his actis scatentem
●Libitum analysis de competitive RNA reticulorum endogenorum (cerna): Providemus altius indaginem in cellularum regulatory mechanisms
●Expertise: with a track record of processing over 20000 samples at BMK, spanning diverse sample types and lncRNA projects, our team brings a wealth of experience to every project.
●Rigorous Quality Control: medias potestates efficiendi puncta per omnes aetates, a praepatione specimen et bibliothecam ad sequencias et bioinformaticas.Haec vigilantia exquisita efficit ut traditionem constanter summus qualitas consequitur.
Post-Sales Support: Munus nostrum ultra project complementum cum 3-mensibus post venditionis tempus servitutis extenditur.Hoc tempore consilium praebemus subsequens, fermentum subsidia, et sessiones Q&A ad quaslibet quaestiones pertinentes ad proventus.
Sample Requisita et Delivery
| Bibliotheca | Platform | Commendatur data | Data QC |
| Poly A locupletatus | Illumina PE150 | 16-20 Gb | Q30≥85% |
Sample Requisita:
Nucleotides:
| Conc. | Moles (μg) | Puritas | Integritas |
| ≥ 100 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Nullam dapibus vel dapibus vel DNA contagione gel ostensum est. | Pro plantis: RIN≥6.5; Pro animalibus: RIN≥7.0; 5.0≥28S/18S≥1.0; limitata vel non collocantur elevatione |
● Plantae:
Radix, Caulis vel Petal: 450 mg
Folium vel Semen: 300 mg
Fructus: 1.2 g
Animal:
Cor vel Intestinum: 450 mg
Viscera vel cerebrum: 240 mg
Musculus: 600 mg
Ossa, capillus vel cutis: 1.5g
Arthropoda:
Insecta: 9g
Crustacea: 450 mg
● totum sanguinem:2 tube
Cellulae: 106 cellulae
Serum et Plasma: 6 mL
Sample Delivery commendatur
Continens: 2 ml tubum centrifugium (foil plumbi non commendatur)
Sample labeling: Group+replicata eg A1, A2, A3;B1, B2, B3...
Shipment:
1. Siccum-glacies: Exemplaria in saccis referta necesse est et in glacie siccitate defossa.
2. RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperie conscendunt.
Service Opus O
Experimentum design
Sample traditio
RNA extractionem
Library construction
Sequencing
Analysis
Post-venditionis officia
Bioinformatics
circRNA praedictio: chromosomatum
Aliter circRNAs Expressit - insidias Volcano
Aliter exprimitur circRNAs - pampineis hierarchicis
Functional locupletatio circRNA exercitum genes
Progressiones investigationes explorare faciliores per BMKGene' circRNA officia sequencia per collectionem publicationum curatarum.
Wang, X. et al.(2021) 'CPSF4 circRNA formationis moderatur et microRNA gene mediata silencium in carcinomate hepatocellulares', Oncogene 2021 40:25, 40(25), pp.doi: 10.1038/s41388-021-01867-6.
Xia, K. et al.(2023) 'X transcriptum oc-responsivum indicat munus circularis RNA133 in morbo resistendi regulando expressionem OsARAB in rice', Phytopathologia Investigationis, 5(1), pp.doi: 10.1186/S42483-023-00188-8/FIGURES/6.
Y, H. et al.(2023) ' CPSF3 stateram circularem et linearem transcriptorum in carcinomate hepatocellulari modulans'.doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al.(2023) 'Aestimatio comprehensiva circRNAs in cirrhotica cardiomyopathy ante et post hepatis transplantatio', International Immunopharmacology, 114, p.109495. doi: 10.1016/J.INTIMP.2022.109495.
