BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Commoda BMKMANU S1000
1) Sub-cellularis resolutio: Singulae areae captae continebant >2 decies centena spatia maculae barcoded cum diametro 2.5 µm et spatium 5 µm inter centra macula, ut analysin transumpto spatio cum resolutione sub-cellulari (5 µm).
2) Analysis resolutio multi-gradua: Flexibilis analysis multi-gradus ab 100 µm ad 5 µm discurrens ad varias textus lineas componendas ad optimam resolutionem.
3) transcriptum comprehensivum profiling: Transumptum captum e toto texturae lapsus resolvi potest, sine restrictione in numero generum scopo et scopo regio.
Service Specifications
| Bibliotheca | Sequencing strategy | Data output commendatae |
| S1000 cDNA library | BMKMANU S1000-Illumina PE150 | 60Gb/sample |
Sample Requisita
| Sample | Numerus | Magnitudo | RNA Quality |
| OCT embedded TEXTUS obstructionum | 2-3 cuneos / sample | Proxime.6.8x6.8x6.8 mm3 | RIN≥7 |
Plura de praeparatione exempli in ductu et servitio workflow, placet liberum loqui ad aBMKGENE expert
Service Opus O
Experimentum design
Sample traditio
Pre-experimentum
Spatium barcoding
Library construction
Sequencing
Analysis
Post-venditionis officia
Notitia a BMKMANU S1000 generata inspicitur usura programmatis "BSTMatrix", quod independenter a BMKGENE designatum est, incluso:
1) matrix generation
II) HE imaginem processus
III) Compatible cum amni tertia-pars software pro analysis
4) Online "BSTViewer" adiuvat ut proventus visualisation in diversis resolutionibus obtineant.
1.Spot pampineis
2.Spatial distribution
Note: Resolutionlevel = 13 (100 µm, left); 7 (50 µm, ius)
3.Marker expressio abundantia pampineis heatmap
4.Inter-specimen analysi
