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Chromatin Transposase accessible per High Throughput Sequencing (ATAC-seq)

ATAC-seq summus est modus sequendi methodus ad analysin accessibilitatis genome-latae chromatinorum, quae magni momenti est pro global epigenetica dicione gene locutionis.adaptatores sequentes in regiones chromatinas apertas ab hyperactivo Tn5 transposasas inseruntur.Post pcr amplificationem, sequentis bibliotheca construitur.Omnes regiones apertae chromatinarum sub certa condicione temporis spatio obtineri possunt, non solum limitata ad situs ligaturae factoris transcriptionis vel regionis histonicae mutationis.


Service Details

Bioinformatics

Demo Results

Servitium Commoda

● Analysis comprehensiva: Analysis professionalis pipeline occurrit variis analysi nativus necessariis;

● Extensiva experientia: Centum inceptorum feliciter perfecit exempla tantum animali et plantae comprehendens.

Service Specifications

Rostra: Illumina NovaSeq platform

Bibliotheca type: ATAC-seq

Commendatur data output: ≥20M legit

Sample Requisita

Sample genus: Tela, cellae vivae, vel cellulae congelatae et sic porro

Cell numerus: ≥ 5×105cellae, numerus cellula accurata clavis successuum experimenti;

TEXTUS pondus: ≥ 200mg textum recens;

Sanguis: ≥ 2 mL

Service Opus O

Sample QC

Experimentum design

Sample partus

Sample traditio

Gubernator experimentum

DNA extractionem

Bibliotheca Praeparatio

Library construction

Sequencing

Sequencing

Analysis

Analysis

Post venditionis Services

Post-venditionis officia


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    1.Heatmap on ATAC legit distributionem in TSS regiones vicinas (±3 kb)

    3(1)2.Distribution of the open chromatin region in different components genome

    4(1)

    3.Differential apicem vocatio inter circulos

    5(1)

    a Quote

    Epistulam tuam hic scribe et mitte nobis

    Epistulam tuam nobis mitte;