10x Genomics Visium Spatial Transcriptome
Features
● Resolutio: 100 µM
Macula Diameter: 55 µM
Maculae: 4992
Captura area: 6.5 x 6.5 mm
● Macula barcoded primers onusta ex 4 sectionibus composita;
- poly(dT) cauda pro mRNA priming et cDNA synthesis
- Unique Identifier (UMI) ad corrigendam amplificationem biam
- Spatial barcode
- Binding sequence of partial read 1 sequencing primer
H & E maculas sectionum
commoda
●Unus-subsisto servitium: omnem experientiam et peritiam fundati gradus integrat, incluso cryo-sectione, maculante, texti optimizationis, spatiis barcoding, bibliothecam praeparationem, sequen- tes et bioinformaticas.
peritissimus technica quadrigis: experientia in supra 250 textuum generibus et 100+ speciebus inter homines, murem, mammalem, pisces et plantas.
●Realis tempus renovatio totius consilii: with full control of experimental progress.
●Commodus comprehensivus bioinformaticus:involucrum includit 29 analyses et 100+ figuras qualitates altas.
●Nativus Analysis et visualisation: available pro diversis petitionibus inquisitionis.
●Ad libitum analysin iuncturam cum Singula cellula variantia sequencing
Specifications
| Sample Requisita | Bibliotheca | Sequencing strategy | Data commendatae | Regimen quālitātis |
| OCT immersa exemplaria cryo, FFPE exempla (Optimal diameter: approx. 6x6x6 mm3) III, caudices per sample | 10X Visium cDNA library | Illumina PE150 | 50K PE legit per macula ( 60Gb ) | RIN>7 |
Plura de praeparatione exempli in regimine et servitio workflow, placet liberum loqui ad aBMKGENE expert
Service Workflow
In praeparatione exempli, mole RNA extractio initialis iudicium conficitur ut RNA summus qualitas obtineatur.In optimizatione textus scaena sectiones maculatae sunt et subjiciuntur ac permeabilizationis condiciones pro variantibus emissione textus optimized sunt.Protocollum optimized tum applicatur in constructione bibliothecae, sequendo sequendo et analysi.
Totum ministerium workflow involvit real-time updates et clientelam confirmationes ad servandum responsativum feedback ansam, ut lenis project exsecutionem procurans.
Sequenti analysi includit:
Data Quality Control:
O data output et qualis score distribution
O gene deprehendatur per maculam
o TEXTUS coverage
Analysis interioris specimen:
o gene divitiae
o Macula pampineis, inter reducta dimensio analysis
O analysis differentialis expressio inter clusters: idem titulus genes
o Functional annotation and locupletation of marking genes .
Inter coetus analysis
o Re- junctura macularum ab utroque exemplari (exempli gratia morbosa et temperantia) et re- glomer
O sativum titulus genes pro singulis botri
o Functional annotation and locupletation of marking genes .
O differentialis elocutio eiusdem botri inter circulos
Interiorem-specimen Analysis
Plowing macula
genesis idem ac loci distributio
Inter coetus Analysis
Data permixtio ex utroque coetu ac rursus glomerus
Genes venalicium novorum clusters
Progressiones explorare faciliores per ministerium spatii BMKGene transcriptomic a 10X Visium In his ephemeridibus divulgandis:
Chen, D. et al.(2023) 'mthl1, Drosophila potentialis homologus adhaesionis mammalium GPCRs, involvit antitumorem reactiones ad cellulas muscas oncogenicas injectas', Acta Nationalis Academiae Scientiarum Americae Foederatae Rei Publicae, 120(30), p.e2303462120.doi: /10.1073/pnas.2303462120
Chen, Y. et al.(2023) FERRUM alta resolutio datorum spatiotemporalium transcriptomic delineationem dat', Briefings in Bioinformatics, 24 (2), pp. 1-10.doi: 10.1093/BIB/BBAD068.
Liu, C. et al.(2022) 'Atlas spatiotemporalis organogenesis in evolutione florum orchidorum', Acides Nucleic Research, 50 (17), pp.doi: 10.1093/NAR/GKAC773.
Wang, J. et al.(2023) 'Integrating Transscriptomics Spatiales et Singulus-nucleus RNA Sequentia Revelat Potentias Therapeuticas Strategies Uterinae Leiomyoma', Acta Internationalis Scientiarum Biologicarum, 19(8), pp.doi: 10.7150/IJBS.83510.
