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T2T GENOME ASSEMBLY, GAP FREE GENOME

1stLoro Genom Beras1

Irah-irahan: Majelis lan Validasi Loro Genom Referensi Bebas Celah kanggo Beras Xian/indica Nyedhiyakake Wawasan babagan Arsitektur Centromere Tanduran

Doi:https://doi.org/10.1101/2020.12.24.424073

Wektu dikirim: 01 Januari 2021.

Institut: Huazhong Agricultural University, China

Bahan

O. sativa xian / indicavarietas padi 'Zhenshan 97 (ZS97)' lan 'Minghui 63 (MH63)

Strategi urutan

NGS diwaca + HiFi diwaca + CLR diwaca + BioNano + Hi-C

data:

ZS97: 8,34 Gb(~23x)HiFi diwaca + 48,39 Gb (~131x) CLR diwaca + 25 Gb(~69x) NGS + 2 sel BioNano Irys

MH63: 37,88 Gb (~103x) HiFi diwaca + 48,97 Gb (~132x) CLR diwaca + 28 Gb(~76x) NGS + 2 sel BioNano Irys

Gambar-1

Gambar 1 Rong genome beras bebas celah (MH63 lan ZS97)

2ndGenom pisang2

Judul: Telomere-to-telomere kromosom tanpa celah pisang nggunakake urutan nanopore

Doi:https://doi.org/10.1101/2021.04.16.440017

Wektu dikirim: April 17, 2021.

Institut: Université Paris-Saclay, Prancis

Bahan

Double haploidMusa acuminatasppmalaccensis(DH-Pahang)

Strategi lan data urutan:

Mode HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ Peta optik (DLE-1+BspQ1)

Tabel 1 Perbandingan rakitan genom Musa acuminata (DH-Pahang).

Tabel1-Perbandingan-GRCh38-lan-T2T-CHM13-majelis-genom-manungsa
Figure-Musa-genom-arsitektur-comparison

Gambar 2. Perbandingan arsitektur genom Musa

3rdGenom Phaeodactylum tricornutum3

Title: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Wektu dikirim: 04 Mei 2021

Institut: Western University, Kanada

Bahan

Phaeodactylum tricornutum(Koleksi Kultur Alga lan Protozoa CCAP 1055/1)

Strategi lan data urutan:

1 sel aliran minION Oxford Nanopore + output mid-end 2×75 pasangan NextSeq 550

Gambar-Alur-kanggo-telomere-kanggo-telomere-genome-assembly-1-1024x740

Gambar 3 Alur kerja kanggo rakitan genom telomere-kanggo-telomere

4thGenom CHM13 manungsa4

Judul: Urutan lengkap genom manungsa

Doi:https://doi.org/10.1101/2021.05.26.445798

Wektu dikirim: 27 Mei 2021

Institut: National Institutes of Health (NIH), USA

Bahan: sel line CHM13

Strategi lan data urutan:

30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), peta optik BioNano, lan Strand-seq

Tabel 2 Perbandingan GRCh38 lan T2T-CHM13 rakitan genom manungsa

Tabel-Bandhingan-Musa-acuminata-DH-Pahang-genome-assemblies

Referensi

1. Sergey Nurk et al.Urutan lengkap génom manungsa.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2. Caroline Belser et al.Telomere-kanggo-telomere kromosom gapless saka pisang nggunakake urutan nanopore.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3. Daniel J. Giguere et al.Rakitan genom telomere-kanggo-telomere saka Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4. Jia-Ming Song et al.Majelis lan Validasi Loro Genom Referensi Bebas Gap kanggo Beras Xian / indica Nyedhiyakake Wawasan babagan Arsitektur Centromere Tanduran.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Wektu kirim: Jan-06-2022

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