Obere usoro RNA-Ilumina
Atụmatụ
● Nha nhọrọ nke RNA tupu nkwadebe ọbá akwụkwọ
● Nyocha bioinformatic gbadoro ụkwụ na amụma miRNA na ebumnuche ha
Uru ọrụ
●Nyocha bioinformatics zuru oke:na-eme ka njirimara nke miRNA ndị ama ama na nke ọhụrụ, njirimara nke ebumnuche miRNA na nkọwa ọrụ kwekọrọ na ịba ụba na ọtụtụ ọdụ data (KEGG, GO)
●Njikwa ogo siri ike: anyị na-emejuputa isi ihe nchịkwa n'ofe niile, site na nlele na nkwadebe ọbá akwụkwọ ruo usoro na bioinformatics.Nleba anya nke ọma a na-eme ka o doo anya na nnyefe nke nsonaazụ dị elu mgbe niile.
●Nkwado maka ire ere: Nkwenye anyị na-agafe karịa mmecha ọrụ yana oge ọrụ ọnwa 3 gachara.N'ime oge a, anyị na-enye nlebanya ọrụ, enyemaka nchọpụta nsogbu, yana oge ajụjụ & A iji zaa ajụjụ ọ bụla metụtara nsonaazụ ya.
●Ọkachamara sara mbara: na ndekọ egwu nke imechi nke ọma n'ọtụtụ ọrụ sRNA na-ekpuchi ụdị 100 na ngalaba nyocha dị iche iche, ndị otu anyị na-eweta ahụmahụ bara ụba na ọrụ ọ bụla.
Ihe nlele chọrọ na nnyefe
| Ụlọ akwụkwọ | Platform | Data akwadoro | Data QC |
| ahọpụtara nha | Illumina SE50 | 10M-20M na-agụ | Q30≥85% |
Ihe atụ chọrọ:
Nucleotides:
| Conc.(ng/μl) | Ọnụ ego (μg) | Ịdị ọcha | Iguzosi ike n'ezi ihe |
| ≥ 80 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Enwere oke ma ọ bụ enweghị protein ma ọ bụ mmetọ DNA nke egosiri na gel. | RIN≥6.5; 5.0≥28S/18S≥1.0; nwere oke ma ọ bụ enweghị mgbago elu |
● Osisi:
Mgbọrọgwụ, azuokokoosisi ma ọ bụ petal: 450 mg
Akwụkwọ ma ọ bụ mkpụrụ: 300 mg
Mkpụrụ: 1.2 g
● Anụmanụ:
Obi ma ọ bụ eriri afọ: 450 mg
Viscera ma ọ bụ ụbụrụ: 240 mg
Akwara: 600 mg
Ọkpụkpụ, ntutu ma ọ bụ akpụkpọ: 1.5g
● Arthropods:
Ụmụ ahụhụ: 9g
Crustacea: 450 mg
● Ọbara dum: 2 tube
● Selụ: 106 mkpụrụ ndụ
● Ọbara na Plasma:6 ml
Nnyefe nlele akwadoro
akpa:
2 ml nke centrifuge tube (anaghị akwado foil tin)
Ịkpọ aha nlele: Otu+ megharịa dịka A1, A2, A3;B1, B2, B3......
Mbupu:
1.Dry-ice: A ga-etinye ihe nlele n'ime akpa ma lie ya na akọrọ-ice.
2.RNAstable tubes: Enwere ike ihichapụ ihe nlele RNA na tube stabilization RNA (dịka RNAstable®) ma bufee ya na okpomọkụ.
Usoro ọrụ ọrụ
Nnwale imewe
Nbufe ihe atụ
mmịpụta RNA
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Mgbe-ire ọrụ
Bioinformatics
Nchọpụta nke miRNA: nhazi na omimi
Okwu dị iche iche nke miRNA - nchịkọta ọkwa
Nkọwa ọrụ nke ebumnuche nke miRNA ndị egosipụtara iche
Nyochaa ọganihu nyocha nke ọrụ nhazi usoro BMKGene'sRNA na-akwado site na nchịkọta mbipụta.
Chen, H. et al.(2023) 'ọrịa nje na-egbochi saponin biosynthesis na photosynthesis na Panax notoginseng', Plant Physiology and Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al.(2023)' Osisi FYVE ngalaba-nwere protein FREE1 na-ejikọta ya na microprocessor components iji mebie miRNA biogenesis', EMBO na-akọ, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al.(2023) 'MicroRNA Ame-Bantam-3p na-achịkwa Larval Pupal Development site n'ịtụle Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) na Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al.(2018) 'Ntụle agbakwunyere nke MiRNA na mkpụrụ ndụ ihe nketa jikọtara ya na ịdị mma anụ na-ekpughe na Gga-MiR-140-5p na-emetụta abụba anụ ahụ intramuscular na ọkụkọ', Physiology Cellular and Biochemistry, 46(6), p. 2421-2433.doi: 10.1159/000489649.
