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Usoro RNA otu nucleus
Ọganihu n'ime otu cell na usoro iwu ụlọ ọba akwụkwọ jikọtara ya na usoro ntinye dị elu na-enye ohere ọmụmụ mkpụrụ ndụ ihe nketa site na cell site na cell.Ọ na-eme ka nyocha nke sistemu dị omimi na nke zuru oke na ọnụ ọgụgụ sel dị mgbagwoju anya, nke ọ na-ezere nkpuchi nke ịdị iche iche ha site na iwere nkezi nke sel niile.
Otú ọ dị, ụfọdụ mkpụrụ ndụ adịghị mma ka e mee ya ka ọ bụrụ nkwusioru nke otu cell, ya mere, usoro nkwadebe ihe atụ ndị ọzọ - a na-ewepụta oghere site na anụ ahụ, ya bụ, a na-esi na anụ ahụ ma ọ bụ cell ewepụta ngwa ngwa ma kwadebe ya n'otu nkwusioru maka otu-nucleus. usoro cell.
BMK na-enye 10× Genomics ChromiumTM nke dabere na otu cell RNA ọrụ usoro.A na-eji ọrụ a eme ihe n'ọtụtụ ebe n'ọmụmụ ihe gbasara ọmụmụ ihe metụtara ọrịa, dị ka ọdịiche dị n'etiti cell cell, ọdịdị nke tumor, mmepe anụ ahụ, wdg.
Mgbawa transcriptome spatial: 10× Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
transcriptomics spatial na-eguzo n'ihu nke mmepụta sayensị, na-enye ndị nchọpụta ike ịbanye n'ime usoro okwu mkpụrụ ndụ mgbagwoju anya n'ime anụ ahụ ma na-echekwa ọnọdụ ha.N'etiti nyiwe dị iche iche, BMKGene ewepụtala BMKManu S1000 Spatial Transcriptome Chip, na-anya isienwekwukwa mkpebinke 5µM, na-eru nso subcellular, ma na-enyere akantọala mkpebi ọtụtụ ọkwa.Ihe mgbawa S1000, nke na-egosipụta ihe dị ka nde ntụpọ abụọ, na-arụ ọrụ microwells nke nwere beads jupụtara na nyocha njide oghere.A na-akwado ọba akwụkwọ cDNA, nke ejiri koodu barcode mejuo ya, site na mgbawa S1000 wee mee ya n'usoro n'elu ikpo okwu Illumina NovaSeq.Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara.Àgwà pụrụ iche nke BMKManu S1000 mgbawa dabere na ike ya, na-enye ntọala mkpebi ọkwa dị iche iche nke enwere ike itinye ya nke ọma na anụ ahụ na ọkwa dị iche iche.Ngbanwe a na-edobe mgbawa ahụ dị ka nhọrọ pụtara ìhè maka ọmụmụ ihe gbasara transcriptomics dị iche iche, na-agba mbọ hụ na mkpokọta oghere nwere obere mkpọtụ.
N'iji BMKManu S1000 mgbawa na teknụzụ transcriptomics ndị ọzọ gbasara ohere, ndị nchọpụta nwere ike nweta nghọta ka mma banyere nhazi oghere nke mkpụrụ ndụ na mmekọrịta dị mgbagwoju anya nke na-eme n'ime anụ ahụ, na-enye nghọta bara uru n'ime usoro ndị na-ebute usoro ndu n'ọtụtụ dị iche iche, gụnyere. oncology, neuroscience, mmepe bayoloji, immunology na botanical ọmụmụ.
Platform: BMKManu S1000 mgbawa na Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
transcriptomics spatial bụ nkà na ụzụ na-emepe emepe nke na-enye ndị nchọpụta ohere inyocha usoro okwu mkpụrụ ndụ ihe nketa n'ime anụ ahụ ma na-echekwa ọnọdụ ha.Otu ikpo okwu siri ike na ngalaba a bụ 10x Genomics Visium yana usoro Illumina.Ụkpụrụ nke 10X Visium dabere na mgbawa pụrụ iche nwere mpaghara ejidere ahaziri ebe etinye akụkụ anụ ahụ.Mpaghara njide a nwere ntụpọ akara, nke ọ bụla dabara na ọnọdụ oghere pụrụ iche n'ime anụ ahụ.A na-edezi ụmụ irighiri ihe RNA ndị ahụ e weghaara si n'anụ ahụ nwere ihe nchọpụta mkpụrụ ndụ pụrụ iche (UMIs) n'oge usoro ntụgharị ntụgharị.Ebe akara koodu na UMI ndị a na-eme ka eserese oghere na ọnụọgụ mkpụrụ ndụ ihe nketa na mkpebi otu cell.Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara.Site n'iji teknụzụ Spatial Transcriptomics a, ndị nchọpụta nwere ike nweta nghọta miri emi banyere nhazi oghere nke mkpụrụ ndụ yana mmekọrịta dị mgbagwoju anya na-eme n'ime anụ ahụ, na-enye nghọta bara uru n'ime usoro ndị na-ebute usoro ndu n'ọtụtụ mpaghara, gụnyere oncology, neuroscience, bayoloji mmepe, immunology. , na ọmụmụ ihe gbasara ahịhịa.
Platform: 10X Genomics Visium na Illumina NovaSeq
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Usoro mRNA dị ogologo-Nanopore
Ọ bụ ezie na usoro mRNA dabere na NGS na-eje ozi dị ka ngwa ọrụ dị iche iche maka ịkọwapụta mkpụrụ ndụ ihe nketa, ịdabere na mkpirisi ọgụgụ na-egbochi ịdị irè ya na nyocha transcript dị mgbagwoju anya.N'aka nke ọzọ, usoro Nanopore na-eji teknụzụ na-agụ ogologo oge, na-eme ka usoro nke transcript mRNA dị ogologo.Usoro a na-eme ka nyocha zuru oke nke splicing ọzọ, njikọ mkpụrụ ndụ ihe nketa, poly-adenylation, na ọnụọgụ nke isoforms mRNA.
Usoro Nanopore na-adabere na nanopore otu molecule ozugbo akara ọkụ eletrik.N'ịbụ ndị na-eduzi proteins moto, DNA nwere eriri abụọ na-ejikọta na protein nanopore agbakwunyere na biofilm, na-atụgharị ka ọ na-agafe na ọwa nanopore n'okpuru ọdịiche voltaji.A na-achọpụta akara ọkụ eletrik pụrụ iche nke ntọala dị iche iche na-emepụta na eriri DNA na nkewa ozugbo, na-eme ka usoro nucleotide ziri ezi na na-aga n'ihu.Ụzọ ọhụrụ a na-emeri njedebe nke ọgụgụ dị mkpirikpi ma na-enye ikpo okwu siri ike maka nyocha mkpụrụ ndụ dị mgbagwoju anya, gụnyere nchọpụta transcript dị mgbagwoju anya.
Platform: Nanopore Promethion P48
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Usoro mRNA zuru oke -PacBio
Ọ bụ ezie na usoro mRNA dabere na NGS bụ ngwá ọrụ dị iche iche maka ịkọwapụta mkpụrụ ndụ ihe nketa, ịdabere na mkpirisi agụ na-egbochi ojiji ya na nyocha transcript dị mgbagwoju anya.PacBio sequencing (Iso-Seq), n'aka nke ọzọ, na-eji teknụzụ na-agụ ogologo oge, na-eme ka usoro nke transcript mRNA zuru oke.Usoro a na-eme ka nyocha zuru oke nke splicing ọzọ, njikọ mkpụrụ ndụ ihe nketa na poly-adenylation ọ bụ ezie na ọ bụghị nhọrọ bụ isi maka ọnụọgụ okwu mkpụrụ ndụ ihe nketa, n'ihi oke data achọrọ.
Teknụzụ usoro PacBio dabere na usoro otu-molecule, ezigbo oge (SMRT), na-enye uru dị iche iche n'inweta transcript mRNA zuru oke.Uzo ohuru ohuru a gunyere iji uzo uzo efu (ZMWs), olulu mmiri microfabricated nke na-enyere aka nleba anya n'oge nke DNA polymerase n'oge usoro.N'ime ZMW ndị a, PacBio's DNA polymerase na-emepụta eriri DNA na-emekọ ihe, na-ewepụta ogologo ọgụgụ nke na-agafe mkpokọta mRNA.Ịrụ ọrụ PacBio na usoro nkwenye nkwekọrịta okirikiri (CCS) na-akwalite izi ezi site n'ịhazi otu molekul ugboro ugboro.Ọgụgụ HiFi emepụtara nwere izi ezi nke atụnyere NGS, na-enye aka n'ihu na nyocha zuru oke na ntụkwasị obi nke njirimara transcriptomic siri ike.Platform: PacBio Sequel II
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Eukaryotic mRNA Sequencing-Ilumina
Usoro mRNA na-enye ike ịkọwapụta nkọwapụta mRNA niile n'ime sel n'okpuru ọnọdụ akọwapụtara.Teknụzụ ọgbara ọhụrụ a na-arụ ọrụ dị ka ngwa ọrụ siri ike, na-ekpughe profaịlụ mkpụrụ ndụ dị mgbagwoju anya, usoro mkpụrụ ndụ ihe nketa, na usoro mkpụrụ ndụ jikọtara ya na usoro ndu dị iche iche.N'ịbụ nke anakwere na nyocha dị mkpa, nchọpụta ụlọ ọgwụ, na mmepe ọgwụ, usoro mRNA na-enye nghọta n'ime mgbagwoju anya nke ike ndụ ekwentị na ụkpụrụ mkpụrụ ndụ ihe nketa.
Platform: Illumina NovaSeq X
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Usoro mRNA na-abụghị nke nrụtụ aka-Ilumina
Usoro mRNA na-enye ike ịkọwapụta nkọwapụta mRNA niile n'ime sel n'okpuru ọnọdụ akọwapụtara.Teknụzụ ọgbara ọhụrụ a na-arụ ọrụ dị ka ngwa ọrụ siri ike, na-ekpughe profaịlụ mkpụrụ ndụ dị mgbagwoju anya, usoro mkpụrụ ndụ ihe nketa, na usoro mkpụrụ ndụ jikọtara ya na usoro ndu dị iche iche.N'ịbụ nke anakwere na nyocha dị mkpa, nchọpụta ụlọ ọgwụ, na mmepe ọgwụ, usoro mRNA na-enye nghọta n'ime mgbagwoju anya nke ike ndụ ekwentị na ụkpụrụ mkpụrụ ndụ ihe nketa.
Platform: Illumina NovaSeq X
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Ogologo usoro enweghị koodu-Ilumina
Ogologo RNA ndị na-abụghị koodu (lncRNAs) bụ RNA ndị toro ogologo karịa 200 nucleotides nwere ike itinye koodu ma bụrụ ihe dị mkpa n'ime RNA na-abụghị koodu.Achọpụtara na nucleus na cytoplasm, ndị RNA ndị a na-arụ ọrụ dị mkpa na epigenetic, transcriptional, na post-transcriptional regulation, na-egosi mkpa ha dị na ịkpụzi usoro cellular na molecular.Usoro LncRNA bụ ngwa ọrụ siri ike na ọdịiche nke mkpụrụ ndụ, Ontogenesis na ọrịa mmadụ.
Platform: Illumina NovaSeq
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Obere usoro RNA-Ilumina
Obere ụmụ irighiri RNA (sRNA), nke na-adịkarị n'okpuru 200 nucleotides n'ogologo, gụnyere microRNAs (miRNAs), obere RNA (siRNAs), na RNA ndị na-emekọrịta ihe (piRNAs).N'ime ndị a, miRNA, gburugburu 20-24 nucleotides ogologo, bụ ihe kwesịrị ịrịba ama maka ọrụ nchịkwa ha dị mkpa na usoro cellular dị iche iche.Site na usoro ngosipụta nke anụ ahụ na nke akọwapụtara nke ọma, miRNA na-egosipụta nchekwa dị elu n'ofe ụdị dị iche iche.
Platform: Illumina NovaSeq
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usoro nke circRNA-Ilumina
okirikiri RNA sequencing (circRNA-seq) bụ profaịlụ na nyochaa okirikiri RNA, otu klas nke RNA molekul na-etolite mechiri emechi n'ihi na-abụghị canonical splicing omume, na-enye RNA ndị a nkwụsi ike.Ọ bụ ezie na egosila ụfọdụ circRNA ka ha na-eme ihe dị ka sponge microRNA, na-achọgharị microRNA ma na-egbochi ha ịhazi mRNA ndị ha lekwasịrị anya, circRNA ndị ọzọ nwere ike imekọrịta ihe na protein, gbanwee mkpụrụ ndụ ihe nketa, ma ọ bụ nwee ọrụ na usoro cellular.Nyocha okwu circRNA na-enye nghọta n'ime ọrụ nhazi nke ụmụ irighiri ihe ndị a na mkpa ha dị na usoro cellular dị iche iche, ọkwa mmepe, na ọnọdụ ọrịa, na-enye aka na nghọta miri emi nke mgbagwoju anya nke ụkpụrụ RNA n'ihe gbasara okwu mkpụrụ ndụ ihe nketa.
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Usoro transcriptome niile - Illumina
Usoro transcriptome niile na-enye ụzọ zuru oke maka ịkọwapụta mkpụrụ ndụ RNA dị iche iche, gụnyere ma nzuzo (mRNA) na RNA ndị na-abụghị koodu (lncRNA, circRNA, na miRNA).Usoro a na-ewepụta transcriptome nke mkpụrụ ndụ kpọmkwem n'otu oge, na-enye ohere maka nghọta zuru oke nke usoro cellular.Amarakwa dị ka “ngụkọta usoro RNA,” ọ na-achọ ikpughe netwọọdụ usoro iwu dị mgbagwoju anya na ọkwa transcriptome, na-enye nyocha miri emi dị ka asọmpi endogenous RNA (ceRNA) na nyocha RNA nkwonkwo.Nke a na-egosi nzọụkwụ mbụ n'ịkọwapụta ọrụ, ọkachasị n'iwepụta netwọkụ nchịkwa metụtara mmekọrịta ceRNA dabere na circRNA-miRNA-mRNA.
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Usoro prokaryotic mRNA
Usoro mRNA na-enye ike ịkọwapụta nkọwapụta mRNA niile n'ime sel n'okpuru ọnọdụ akọwapụtara.Teknụzụ ọgbara ọhụrụ a na-arụ ọrụ dị ka ngwa ọrụ siri ike, na-ekpughe profaịlụ mkpụrụ ndụ dị mgbagwoju anya, usoro mkpụrụ ndụ ihe nketa, na usoro mkpụrụ ndụ jikọtara ya na usoro ndu dị iche iche.N'ịbụ nke anakwere na nyocha dị mkpa, nchọpụta ụlọ ọgwụ, na mmepe ọgwụ, usoro mRNA na-enye nghọta n'ime mgbagwoju anya nke ike ndụ ekwentị na ụkpụrụ mkpụrụ ndụ ihe nketa.A haziri nhazi nlele prokaryotic mRNA anyị maka transcriptomes prokaryotic, gụnyere mbelata rRNA na nkwadebe ọbá akwụkwọ ntụzịaka.
Platform: Illumina NovaSeq X
