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Usoro RNA otu nucleus
Ọganihu n'ime otu cell na usoro iwu ụlọ ọba akwụkwọ jikọtara ya na usoro ntinye dị elu na-enye ohere ọmụmụ mkpụrụ ndụ ihe nketa site na cell site na cell.Ọ na-eme ka nyocha nke sistemu dị omimi na nke zuru oke na ọnụ ọgụgụ sel dị mgbagwoju anya, nke ọ na-ezere nkpuchi nke ịdị iche iche ha site na iwere nkezi nke sel niile.
Otú ọ dị, ụfọdụ mkpụrụ ndụ adịghị mma ka e mee ya ka ọ bụrụ nkwusioru nke otu cell, ya mere, usoro nkwadebe ihe atụ ndị ọzọ - a na-ewepụta oghere site na anụ ahụ, ya bụ, a na-esi na anụ ahụ ma ọ bụ cell ewepụta ngwa ngwa ma kwadebe ya n'otu nkwusioru maka otu-nucleus. usoro cell.
BMK na-enye 10× Genomics ChromiumTM nke dabere na otu cell RNA ọrụ usoro.A na-eji ọrụ a eme ihe n'ọtụtụ ebe n'ọmụmụ ihe gbasara ọmụmụ ihe metụtara ọrịa, dị ka ọdịiche dị n'etiti cell cell, ọdịdị nke tumor, mmepe anụ ahụ, wdg.
Mgbawa transcriptome spatial: 10× Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
transcriptomics spatial na-eguzo n'ihu nke mmepụta sayensị, na-enye ndị nchọpụta ike ịbanye n'ime usoro okwu mkpụrụ ndụ mgbagwoju anya n'ime anụ ahụ ma na-echekwa ọnọdụ ha.N'etiti nyiwe dị iche iche, BMKGene ewepụtala BMKManu S1000 Spatial Transcriptome Chip, na-anya isienwekwukwa mkpebinke 5µM, na-eru nso subcellular, ma na-enyere akantọala mkpebi ọtụtụ ọkwa.Ihe mgbawa S1000, nke na-egosipụta ihe dị ka nde ntụpọ abụọ, na-arụ ọrụ microwells nke nwere beads jupụtara na nyocha njide oghere.A na-akwado ọba akwụkwọ cDNA, nke ejiri koodu barcode mejuo ya, site na mgbawa S1000 wee mee ya n'usoro n'elu ikpo okwu Illumina NovaSeq.Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara.Àgwà pụrụ iche nke BMKManu S1000 mgbawa dabere na ike ya, na-enye ntọala mkpebi ọkwa dị iche iche nke enwere ike itinye ya nke ọma na anụ ahụ na ọkwa dị iche iche.Ngbanwe a na-edobe mgbawa ahụ dị ka nhọrọ pụtara ìhè maka ọmụmụ ihe gbasara transcriptomics dị iche iche, na-agba mbọ hụ na mkpokọta oghere nwere obere mkpọtụ.
N'iji BMKManu S1000 mgbawa na teknụzụ transcriptomics ndị ọzọ gbasara ohere, ndị nchọpụta nwere ike nweta nghọta ka mma banyere nhazi oghere nke mkpụrụ ndụ na mmekọrịta dị mgbagwoju anya nke na-eme n'ime anụ ahụ, na-enye nghọta bara uru n'ime usoro ndị na-ebute usoro ndu n'ọtụtụ dị iche iche, gụnyere. oncology, neuroscience, mmepe bayoloji, immunology na botanical ọmụmụ.
Platform: BMKManu S1000 mgbawa na Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
transcriptomics spatial bụ nkà na ụzụ na-emepe emepe nke na-enye ndị nchọpụta ohere inyocha usoro okwu mkpụrụ ndụ ihe nketa n'ime anụ ahụ ma na-echekwa ọnọdụ ha.Otu ikpo okwu siri ike na ngalaba a bụ 10x Genomics Visium yana usoro Illumina.Ụkpụrụ nke 10X Visium dabere na mgbawa pụrụ iche nwere mpaghara ejidere ahaziri ebe etinye akụkụ anụ ahụ.Mpaghara njide a nwere ntụpọ akara, nke ọ bụla dabara na ọnọdụ oghere pụrụ iche n'ime anụ ahụ.A na-edezi ụmụ irighiri ihe RNA ndị ahụ e weghaara si n'anụ ahụ nwere ihe nchọpụta mkpụrụ ndụ pụrụ iche (UMIs) n'oge usoro ntụgharị ntụgharị.Ebe akara koodu na UMI ndị a na-eme ka eserese oghere na ọnụọgụ mkpụrụ ndụ ihe nketa na mkpebi otu cell.Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara.Site n'iji teknụzụ Spatial Transcriptomics a, ndị nchọpụta nwere ike nweta nghọta miri emi banyere nhazi oghere nke mkpụrụ ndụ yana mmekọrịta dị mgbagwoju anya na-eme n'ime anụ ahụ, na-enye nghọta bara uru n'ime usoro ndị na-ebute usoro ndu n'ọtụtụ mpaghara, gụnyere oncology, neuroscience, bayoloji mmepe, immunology. , na ọmụmụ ihe gbasara ahịhịa.
Platform: 10X Genomics Visium na Illumina NovaSeq
