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Illumina na BGI
Teknụzụ usoro nke Illumina, dabere na Sequencing site Synthesis (SBS), bụ ihe ọhụrụ NGS nabatara n'ụwa niile, na-ahụ maka imepụta ihe karịrị 90% nke data usoro usoro ụwa.Ụkpụrụ nke SBS na-agụnye ịse foto n'ụdị fluorescent nke a na-akpọ reversible terminators ka agbakwunyere dNTP ọ bụla, ma mesịa kewaa iji kwe ka ntinye nke ntọala na-esote.N'ebe dNTP anọ niile na-atụgharị akwụsị akwụsị dị na usoro usoro nke ọ bụla, asọmpi eke na-ebelata mbuso agha.Teknụzụ a na-agbanwe agbanwe na-akwado ma ụlọ akwụkwọ ọbá akwụkwọ na-agụ otu na nke jikọtara ọnụ, na-enye ọtụtụ ngwa genomic.Ikike dị elu nke Illumina sequencing na idobe ya dị ka isi nkuku na nyocha nke genomics, na-enye ndị ọkà mmụta sayensị ike ka ha kpughee mgbagwoju anya nke genome nwere nkọwa na arụmọrụ enweghị atụ.
DNBSEQ, nke BGI mebere, bụ teknụzụ NGS ọzọ na-emepe emepe nke jisiri ike belata n'ihu ọnụ ahịa usoro na ịbawanye mmepụta.Nkwadebe nke ọba akwụkwọ DNBSEQ gụnyere nkewa DNA, nkwadebe nke ssDNA na mmụba okirikiri iji nweta DNA nanoballs (DNB).A na-ebunye ihe ndị a n'elu elu siri ike ma mechaa soro ya site na ngwakọta Probe-Anchor Synthesis (cPAS).
Ọrụ usoro ọba akwụkwọ anyị emeburu na-enyere ndị ahịa aka ịkwado ọba akwụkwọ ha si n'ụdị dị iche iche (mRNA, genome dum, amplicon, na ndị ọzọ).N'ikpeazụ, enwere ike ibuga ọba akwụkwọ ndị a gaa na ebe a na-eme usoro anyị maka njikwa mma na usoro n'usoro Illumina ma ọ bụ BGI.
