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Usoro mgbochi mgbochi Chromatin (ChiP-seq)
ChIP-Seq na-enye nkọwapụta genome-obosara nke ebumnuche DNA maka mgbanwe histone, ihe odide ederede, yana protein ndị ọzọ metụtara DNA.Ọ na-ejikọta nhọrọ nke chromatin immuno-precipitation (ChiIP) iji nwetaghachi ogige protein-DNA kpọmkwem, yana ike nke ọgbọ na-abịa (NGS) maka usoro ntinye dị elu nke DNA enwetara.Na mgbakwunye, n'ihi na a na-anata ogige protein-DNA site na mkpụrụ ndụ dị ndụ, enwere ike iji saịtị jikọtara ya na ụdị cell na anụ ahụ dị iche iche, ma ọ bụ n'okpuru ọnọdụ dị iche iche.Ngwa sitere na ụkpụrụ ntụgharị gaa na ụzọ mmepe gaa na usoro ọrịa na karịa.
Platform: Illumina NovaSeq Platform
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Usoro metagenomic -NGS
Metagenome na-ezo aka mkpokọta ihe mkpụrụ ndụ ihe nketa nke obodo agwakọta dị ka metagenome gburugburu ebe obibi, metagenome mmadụ, wdg.Usoro metagenomic bụ ngwá ọrụ molekụla eji enyocha ihe agwakọta genomic nke ewepụtara site na nlele gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta yana ihe gburugburu ebe obibi.
Platform:Illumina NovaSeq Platform
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Usoro Metagenomic-Nanopore
Metagenomics bụ ngwá ọrụ molekụla eji nyochaa ihe ngwakọta genomic nke ewepụtara site na ihe atụ gburugburu ebe obibi, nke na-enye ozi zuru ezu na ụdị dị iche iche na ụbara, nhazi ọnụ ọgụgụ mmadụ, mmekọrịta phylogenetic, mkpụrụ ndụ ihe nketa na-arụ ọrụ na netwọk mmekọrịta na ihe gburugburu ebe obibi, wdg. Nanopore usoro nyiwe ka ewebatala nso nso a. na ọmụmụ metagenomic.Ọrụ ya pụtara ìhè n'ogologo ọgụgụ na-akwalite nyocha nke metagenomic iyi, ọkachasị mgbakọ metagenome.N'iji uru nke ogologo ọgụgụ ọgụgụ, ọmụmụ metagenomic dabere na Nanopore na-enwe ike nweta mgbakọ na-aga n'ihu na-atụnyere metagenomics egbe.E bipụtala ya na metagenomics dabere na Nanopore rụpụtara nke ọma na mkpụrụ ndụ nje mechiri emechi site na microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Platform:Nanopore PromethION P48
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Usoro genome bisulfite dum
DNA methylation na ọnọdụ nke ise na cytosine (5-mC) nwere mmetụta dị mkpa na mkpụrụ ndụ ihe nketa na ọrụ cellular.Ejikọtala ụkpụrụ methylation na-adịghị mma na ọtụtụ ọnọdụ na ọrịa, dị ka ọrịa kansa.WGBS abụrụla ọkọlọtọ ọla edo maka ịmụ methylation genome-wide n'otu mkpebi ntọala.
Platform: Illumina NovaSeq Platform
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Nyocha maka ntụgharị-Chromatin nwere ike ịnweta ya na usoro ntinye dị elu (ATAC-seq)
ATAC-seq bụ usoro nhazi nke dị elu maka nyocha nke nnweta chromatin genome, nke dị mkpa maka njikwa epigenetic zuru ụwa ọnụ nke mkpụrụ ndụ ihe nketa.A na-etinye ihe nkwụnye usoro n'ime mpaghara chromatin mepere emepe site na transposase Tn5 hyperactive.Mgbe PCR nwechara, a na-arụ ọba akwụkwọ nke usoro.Enwere ike nweta mpaghara chromatin niile mepere emepe n'okpuru ọnọdụ oge ohere, ọ bụghị naanị na saịtị njikọ nke ihe odide ederede, ma ọ bụ mpaghara gbanwetụrụ na histone.
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16S/18S/ITS Amplicon Sequencing-PacBio
Nke a dị na 16S na 18S rRNA nwere ma mpaghara echekwara nke ukwuu yana hyper-variable bụ akara mkpisiaka mkpụrụ ndụ zuru oke maka njirimara prokaryotic na eukaryotic.Na-ewere uru nke sequencing, ndị a amplicons nwere ike ezubere iche dabere na ndị echekwara akụkụ na hyper-variable mpaghara nwere ike n'ụzọ zuru ezu ji maka microbial njirimara na-enye aka ọmụmụ ekpuchi microbial diversity analysis, taxonomy, phylogeny, wdg Single-molecule ozugbo (SMRT) ) usoro nke PacBio ikpo okwu na-enyere aka inweta nke ukwuu ziri ezi ogologo agụ, nke nwere ike na-ekpuchi zuru-ogologo amplicon (ihe dị ka 1.5 Kb).Echiche sara mbara nke mkpụrụ ndụ ihe nketa mere ka mkpebi siri ike dị na nkọwa ụdịdị na nje bacteria ma ọ bụ fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing bu n'obi ikpughe phylogeny, taxonomy, na ụdị dị ukwuu n'ime obodo microbial site na nyocha ngwaahịa PCR nke ihe nrịbama mkpụrụ ndụ ihe nketa nke nwere ma akụkụ a kparịtara ụka na nke ukwuu.Iwebata akara mkpisi aka molikula zuru oke site na Woeses et al, (1977) na-enye ike ịkọwapụta microbiome na-enweghị ikewapụ.Usoro nke 16S (bacteria), 18S (fungi) na Internal transcripted spacer(ITS, fungi) na-enye ohere ka a mata ụdị ma ụdị dị ukwuu yana ụdị dị ụkọ na nke amabeghị.Teknụzụ a aghọwo ihe a na-etinye n'ọtụtụ ebe na ngwá ọrụ bụ isi n'ịchọpụta ihe mejupụtara microbial dị iche iche na gburugburu ebe dị iche iche, dị ka ọnụ mmadụ, eriri afọ, nsị, wdg.
Platform:Illumina NovaSeq Platform
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Usoro njegharị nje na fungal dum Genome
Bacterial na fungal dum genome re-sequencing bụ ngwá ọrụ dị oke mkpa iji mezue genomes nke nje bacteria na fungi a ma ama, yana iji tụnyere ọtụtụ genome ma ọ bụ iji map genomes nke ihe ọhụrụ dị ndụ.Ọ dị oke mkpa ịhazi usoro genome niile nke nje bacteria na fungi iji mepụta mkpụrụ ndụ ihe nketa ziri ezi, mee nchọpụta microbial na ọmụmụ ihe ọmụmụ genome ndị ọzọ.
Platform: Illumina NovaSeq Platform
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PacBio-Uni ogologo 16S/18S/ITS Usoro Amplicon
Amplicon (16S / 18S / ITS) n'elu ikpo okwu na-mepụtara na afọ nke ahụmahụ na microbial diversity oru ngo analysis, nke nwere ọkọlọtọ isi analysis na ahaziri analysis: isi analysis na-ekpuchi mainstream analysis ọdịnaya nke ugbu a microbial nnyocha, na analysis ọdịnaya bụ ọgaranya na keukwu. na nsonaazụ nyocha gosipụtara n'ụdị akụkọ akụkọ;Ọdịnaya nke nyocha ahaziri iche dị iche iche.Enwere ike ịhọpụta ihe nlele ma nwee ike ịtọ ntọala n'ụzọ dị nro dị ka akụkọ nyocha na ebumnuche nyocha siri dị, iji ghọta ihe achọrọ ahaziri iche.Sistemụ arụmọrụ Windows, dị mfe na ngwa ngwa.
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Transcriptome-ogologo zuru oke nke PacBio (anaghị ntụnye aka)
Inweta data sequencing nke Pacific Biosciences (PacBio) dị ka ntinye, ngwa a nwere ike ịchọpụta usoro transcript zuru oke (na-enweghị mgbakọ).Site na ịdepụta usoro ogologo zuru oke megide genome ntụaka, enwere ike ịhazi transcript site na mkpụrụ ndụ amaara ama, transcripts, mpaghara koodu, wdg. N'okwu a, enwere ike nweta njirimara ziri ezi nke ihe owuwu mRNA, dị ka splicing ọzọ, wdg.Nyocha ọnụ na NGS transcriptome sequencing data na-enyere nkọwa zuru ezu na nkọwa zuru oke na okwu na ọkwa transcript, nke na-erite uru dị ukwuu n'okwu dị iche iche na nyocha ọrụ.
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Mbelata Nnọchiteanya Bisulfite Sequencing (RRBS)
Nchọpụta methylation DNA na-abụkarị isiokwu na-ekpo ọkụ na nyocha ọrịa, yana njikọ chiri anya na okwu mkpụrụ ndụ ihe nketa na ụdị pheno.RRBS bụ usoro ziri ezi, dị mma na nke akụ na ụba maka nyocha DNA methylation.Ịba ụba nke mpaghara ndị na-akwalite na CpG agwaetiti site na enzymatic cleavage (Msp I), jikọtara ya na usoro Bisulfite, na-enye nchọpụta DNA methylation dị elu.
Platform: Illumina NovaSeq Platform
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Usoro prokaryotic mRNA
Usoro mRNA na-enye ike ịkọwapụta nkọwapụta mRNA niile n'ime sel n'okpuru ọnọdụ akọwapụtara.Teknụzụ ọgbara ọhụrụ a na-arụ ọrụ dị ka ngwa ọrụ siri ike, na-ekpughe profaịlụ mkpụrụ ndụ dị mgbagwoju anya, usoro mkpụrụ ndụ ihe nketa, na usoro mkpụrụ ndụ jikọtara ya na usoro ndu dị iche iche.N'ịbụ nke anakwere na nyocha dị mkpa, nchọpụta ụlọ ọgwụ, na mmepe ọgwụ, usoro mRNA na-enye nghọta n'ime mgbagwoju anya nke ike ndụ ekwentị na ụkpụrụ mkpụrụ ndụ ihe nketa.A haziri nhazi nlele prokaryotic mRNA anyị maka transcriptomes prokaryotic, gụnyere mbelata rRNA na nkwadebe ọbá akwụkwọ ntụzịaka.
Platform: Illumina NovaSeq X
