transcriptomics spatial na-eguzo n'ihu nke mmepụta sayensị, na-enye ndị nchọpụta ike ịbanye n'ime usoro okwu mkpụrụ ndụ mgbagwoju anya n'ime anụ ahụ ma na-echekwa ọnọdụ ha.N'etiti nyiwe dị iche iche, BMKGene ewepụtala BMKManu S1000 Spatial Transcriptome Chip, na-anya isienwekwukwa mkpebinke 5µM, na-eru nso subcellular, ma na-enyere akantọala mkpebi ọtụtụ ọkwa.Ihe mgbawa S1000, nke na-egosipụta ihe dị ka nde ntụpọ abụọ, na-arụ ọrụ microwells nke nwere beads jupụtara na nyocha njide oghere.A na-akwado ọba akwụkwọ cDNA, nke ejiri koodu barcode mejuo ya, site na mgbawa S1000 wee mee ya n'usoro n'elu ikpo okwu Illumina NovaSeq.Nchikota nke ihe nlele oghere na UMI na-eme ka o doo anya na izi ezi na nkọwapụta nke data ewepụtara.Àgwà pụrụ iche nke BMKManu S1000 mgbawa dabere na ike ya, na-enye ntọala mkpebi ọkwa dị iche iche nke enwere ike itinye ya nke ọma na anụ ahụ na ọkwa dị iche iche.Ngbanwe a na-edobe mgbawa ahụ dị ka nhọrọ pụtara ìhè maka ọmụmụ ihe gbasara transcriptomics dị iche iche, na-agba mbọ hụ na mkpokọta oghere nwere obere mkpọtụ.
N'iji BMKManu S1000 mgbawa na teknụzụ transcriptomics ndị ọzọ gbasara ohere, ndị nchọpụta nwere ike nweta nghọta ka mma banyere nhazi oghere nke mkpụrụ ndụ na mmekọrịta dị mgbagwoju anya nke na-eme n'ime anụ ahụ, na-enye nghọta bara uru n'ime usoro ndị na-ebute usoro ndu n'ọtụtụ dị iche iche, gụnyere. oncology, neuroscience, mmepe bayoloji, immunology na botanical ọmụmụ.
Platform: BMKManu S1000 mgbawa na Illumina NovaSeq