GENOME EVOLUTION
Genome De novo
Mgbakọ|Mkpebi nwoke na nwanyị bụ Biomarker Techonologies nyere ọrụ nhazi usoro yana ọrụ bioinformatic akụkụ.
Abstract
"The iconic phenotype nke seadragons na-agụnye ngwa ngwa dị ka akwụkwọ, ọnụ tubular ezé na-enweghị eze, na ime nwoke nke gụnyere incubation nke àkwá fatịlaịza n'ime oghere "patch" mepere emepe.Anyị de novo-usoro nke nwoke na nwanyị genome nke nkịtị seadragon (Phyllopteryx taeniolatus) na ụdị ya nwere njikọ chiri anya, alligator pipefish (Syngnathoides biculeatus).Profaịlụ ndegharị sitere na ihe ọhụrụ evolushọn, ihe mgbakwunye dị ka akwụkwọ, na-egosi na ejikọtala usoro mkpụrụ ndụ ihe nketa na-etinyekarị aka na mmepe fin yana ịba ụba nke ederede tran maka mmezi anụ ahụ na mkpụrụ ndụ ihe nchebe nchebe.Achọpụtara zebrafish mutants maka scpp5, nke na-efunahụ na syngnathids niile, na-enweghị ma ọ bụ nwere ezé pharyngeal mebiri emebi, na-akwado echiche na ọnwụ nke scpp5 emewo ka ọkpụkpụ ezé na syngnathids.Achọpụtara nwoke na nwanyị na-edobe anya - na-achọpụta ebe na-etinye mkpụrụ ndụ amhr2y kpọmkwem nke nwoke nke sedragon nkịtị na pipefish na-ekekọrịta. ”
Akụrụngwa na ụzọ:
Akụrụngwa
Fsedragon anyị nkịtị (P. taeniolatus) na pipefish abụọ (S. biaculeatus) ụdị.A na-eji seadragon abụọ (otu nwoke na otu nwanyị) na pipefish abụọ (otu nwoke na otu nwanyị) ndị mmadụ mee ihe maka usoro genome dum, na ndị ikom abụọ ndị ọzọ na-emekarị seadragon ndị ọzọ na-eji genome resequencing.
Atụmatụ usoro nke genome
2Ọbá akwụkwọ 70/350 bp (Hiseq 2500) + Pacbio 20Kb (Sequel) ma ọ bụ Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .sequencing, na ndị ikom abụọ ọzọ na-ahụkarị seadragon ndị e ji mee ihe maka genome resequencing.
Usoro transcriptome
Fma ọ bụ seadragon nkịtị, ụbụrụ, anya, gill, imeju, obi, akụrụ, mesentery, eriri afọ, mọzụlụ, ntù, anụ ahụ, akwụkwọ ndị yiri appendages, testis, na ovary.
Genome de novo mgbakọ atụmatụ
Canu (Ndozi) + WTDBG2 (Mgbakọ) + Pilon (Polish) + Lachesis (Hi-C).
Isi nsonaazụ
Fig. 1. Ihe ndị dị mkpa nke seadragon nkịtị (P. taeniolatus) na pipefish (S. biculeatus) na ọnọdụ phylogenetic ha.
Fig. 2. Morphology na mkpụrụ ndụ ihe nketa nke akwukwo-dị ka appendages na nkịtị seadragon (P. taeniolatus).
Fig. 3. Putative mmekọahụ determination gene in the common seadragon (P. taeniolatus) na alligator pipefish (S. biaculeatus).
Fig. 4. Pharyngeal eze phenotypes na zebrafish scpp5 homozygous mutants.
Oge nzipu: Jan-08-2022